2008
DOI: 10.1128/jb.01757-07
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Abstract: Pseudomonas syringae causes plant diseases, and the main virulence mechanism is a type III secretion system (T3SS) that translocates dozens of effector proteins into plant cells. Here we report the existence of a subgroup of P. syringae isolates that do not cause disease on any plant species tested. This group is monophyletic and most likely evolved from a pathogenic P. syringae ancestor through loss of the T3SS. In the nonpathogenic isolate P. syringae 508 the genomic region that in pathogenic P. syringae str… Show more

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Cited by 66 publications
(86 citation statements)
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“…The 40 strains of P. syringae unable to incite a hypersensitive reaction used for this study were selected from that survey to represent the range of sample sites, substrates and phylogenetic diversity. Reference strains included the HR À strains Psy508 and Psy642 (Mohr et al, 2008;Clarke et al, 2010) and the HR þ strains B728A (from the stock culture of S Hirano, University of Wisconsin, Madison, WI, USA), DC3000 and 1448A from J Greenberg (The University of Chicago), and Cit7 from SE Lindow (University of California, Berkeley, CA, USA). All strains were stored at À80 1C in 20% glycerol, and all phenotypic and molecular tests were conducted on cultures prepared from a single transfer of the storage cultures.…”
Section: Methodsmentioning
confidence: 99%
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“…The 40 strains of P. syringae unable to incite a hypersensitive reaction used for this study were selected from that survey to represent the range of sample sites, substrates and phylogenetic diversity. Reference strains included the HR À strains Psy508 and Psy642 (Mohr et al, 2008;Clarke et al, 2010) and the HR þ strains B728A (from the stock culture of S Hirano, University of Wisconsin, Madison, WI, USA), DC3000 and 1448A from J Greenberg (The University of Chicago), and Cit7 from SE Lindow (University of California, Berkeley, CA, USA). All strains were stored at À80 1C in 20% glycerol, and all phenotypic and molecular tests were conducted on cultures prepared from a single transfer of the storage cultures.…”
Section: Methodsmentioning
confidence: 99%
“…For detection of hrp/hrc cluster genes (hrpK1, hrpL, hrcC), CEL effectors (avrE1, hrpW1) and hopI1, primers described by Mohr et al (2008) were used for amplification, whereas for phage detection we designed forward primer PhF (5 0 -TCAGGCCGCT TTCAACTTGGC-3 0 ) and reverse primer PhR (5 0 -CCA TAYGGCGCAGTGGTGG-3 0 ) based on the sequence of a putative transcriptional regulator gene (locus tag ABX64468) located within the prophage at the hrp/hrc locus of Psy508 (Mohr et al, 2008). To detect the presence of the hrcC ortholog of Psy642, colony PCR was performed as previously described (Clarke et al, 2010).…”
Section: Methodsmentioning
confidence: 99%
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