2005
DOI: 10.1128/cdli.12.8.930-934.2005
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Abstract: Healthy human sera (HHS) contain naturally acquired enterococcal antibodies which promote neutrophilmediated killing. The target antigens remain unknown. The present study used a capsular polysaccharide (CPS)-enzyme-linked immunosorbent assay (ELISA) to investigate whether the HHS antibodies of 12 healthy donors bound to the CPS of four E. faecalis serotypes (CPS-A to CPS-D) and then employed an opsonic-killing assay to determine if these antibodies mediated phagocyte-dependent killing. All HHS contained immun… Show more

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Cited by 24 publications
(28 citation statements)
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“…Serotypes A and B (containing Maekawa type 1) are nonencapsulated and contain only cpsA and cpsB. Serotype C (including Maekawa type 2) contains cpsC to cpsK, while serotype D (containing Maekawa type 5) is like serotype C but also lacks cpsF (23,25,29,30). Also of interest, isolates of Maekawa type 2 have been noted for harboring more virulence and antibiotic resistance traits than the other types (27).…”
mentioning
confidence: 99%
See 1 more Smart Citation
“…Serotypes A and B (containing Maekawa type 1) are nonencapsulated and contain only cpsA and cpsB. Serotype C (including Maekawa type 2) contains cpsC to cpsK, while serotype D (containing Maekawa type 5) is like serotype C but also lacks cpsF (23,25,29,30). Also of interest, isolates of Maekawa type 2 have been noted for harboring more virulence and antibiotic resistance traits than the other types (27).…”
mentioning
confidence: 99%
“…The upstream genes cpsA and cpsB (present in all E. faecalis strains and initially thought to be involved in capsule production) are transcribed from a different promoter and are not part of the operon (28). Furthermore, pre-vious studies classified E. faecalis isolates into four serotypes, serotypes A to D, which include the Maekawa serotyping strains T1, T2, and T5, representing the three prototypical capsule locus polymorphisms (29). Serotypes A and B (containing Maekawa type 1) are nonencapsulated and contain only cpsA and cpsB.…”
mentioning
confidence: 99%
“…Studies based on the serotyping scheme proposed by Hufnagel et al (17) have shown that serotype C and D strains are much more resistant to opsonophagoctyosis by neutrophils in the presence of normal human serum. More recently, a study by McBride et al indicated that serotype C clinical isolates harbored a greater repertoire of antibiotic resistance cassettes and were more likely to possess multiple virulence factors than the other serotypes, suggesting that the presence of the capsule is associated with pathogenic lineages of E. faecalis (17,24).…”
mentioning
confidence: 99%
“…Studies based on the serotyping scheme proposed by Hufnagel et al (17) have shown that serotype C and D strains are much more resistant to opsonophagoctyosis by neutrophils in the presence of normal human serum. More recently, a study by McBride et al indicated that serotype C clinical isolates harbored a greater repertoire of antibiotic resistance cassettes and were more likely to possess multiple virulence factors than the other serotypes, suggesting that the presence of the capsule is associated with pathogenic lineages of E. faecalis (17,24).It is essential to understand the underlying mechanisms of capsule production in E. faecalis because of ongoing efforts to develop alternative therapies targeting capsule. Here, we used a novel vector system for creating isogenic, in-frame deletion mutants to analyze the genetic basis for capsule production and serotype specificity.…”
mentioning
confidence: 99%
“…As a result, passive immunization of anti-diheteroglycan antibodies successfully protected CPS-C and CPS-D E. faecalis bacteremia mouse model. However, it was observed that considerably lower susceptibility of CPS-C and CPS-D strains to opsonic killing by naturally acquired antibodies was present in healthy human sera as compared with CPS-A and CPS-B [111]. Therefore, capsule may be a natural barrier to access therapeutic antibody by masking antigens underneath.…”
Section: Polysaccharide Antigens As Antibody Targetsmentioning
confidence: 94%