Natural nodulation of Acacia mangium–Acacia auriculiformis hybrids: distribution of the indigenous strains in the nodules

Samba, Ramatoulaye; Neyra, Marc; Lesueur, Didier

doi:10.1007/s11274-007-9373-z

Cited by 2 publications

(1 citation statement)

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“…Acacia mangium × A. auriculiformis, a hybrid of A. mangium and A. auriculiformis [1], is a promising tree species with superior characteristics of growth, adaptability, ber quality, pulping properties, and insect and wind resistance compared to the parental species [2][3][4][5][6]. However, asexual propagation is the only way to maintain superiority [7,8].…”

Section: Introductionmentioning

confidence: 99%

Acacia Mangium × A. Auriculiformis Micropropagation in a Non-Sterile Environment

LieJian

Hong

et al. 2021

Preprint

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Background: Autoclaving is used to eliminate contamination during tissue culturing, however, it is a complicated process, time-consuming and costly. Chemical sterilization of tissue culture can effectively eliminate contamination, is a simple procedure, and cost effective. However, studies on the chemical sterilization mostly focus on bud induction, while the effects of chemical sterilization overall process of tissue culture, including bud induction, proliferation, and rooting, remain to be determined. Here, we investigate the effect of chemical sterilization on bud induction, proliferation, and rooting of Acacia mangium × A. auriculiformis.Results: The results showed that chlorothalonil (0.2 g/L) was a suitable chemical sterilant, and bud induction medium was 1/8 Murashige and Skoog medium + agar 7 g/L + chlorothalonil 0.2 g/L + 6-benzylaminopurine 0.5 mg/L The highest induction rate (99.54%) was observed in the third to fifth buds’ stem segments collected in October treated with 0.8 g/L carbendazim for 3 min, with a contamination rate of 0. The rooting medium was agar 7 g/L + chlorothalonil 0.2 g/L+ indolebutyric acid 1.5 mg/L + naphthylacetic acid 0.5 mg/L, and the rooting rate was 97.62%. The proliferation rate and subculture duration showed a positive correlation, while the proliferation rate was 3.58 times higher at the fourth subculture rooting. Conclusions: Our results suggest that chlorothalonil can effectively replace autoclaving during bud induction, proliferation, and rooting of A. mangium × A. auriculiformis. The findings of this study provide technical support for rapid seedlings propagation, accelerates the breeding process of Acacia, and can be applied in other tree species.

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