Natural infectivity of Anopheles species from the Pacific and Atlantic Regions of Colombia

Gutiérrez, Luis Felipe Higuita; Naranjo-Díaz, Nelson; Jaramillo, Luz; Muskus, Carlos; Luckhart, Shirley; Conn, Jan E.; Correa, Margarita M.

doi:10.1016/j.actatropica.2008.04.019

Cited by 69 publications

(91 citation statements)

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“…These low frequencies were expected given the ecological characteristics of the locations sampled, as A. albimanus is predominantly found at lower altitudes and on coastal areas, preferring slightly brackish larval habitats (5,15). However, some authors have shown the presence of A. albimanus in higher altitudes and non-costal localities (27,28), which suggests adaptability of this species to other ecosystems.…”

Section: Discussionmentioning

confidence: 83%

“…Localities were selected based on official reports of malaria cases and the geographic distribution of malaria vectors (15,16).…”

Section: Study Areamentioning

confidence: 99%

“…We conducted an enzyme-linked immune-sorbent assay (ELISA) for Plasmodium detection following standardized protocols (15,16). Monoclonal antibodies (MAb) were provided by the Centers for Disease Control and Prevention (CDC, Atlanta) (19)(20)(21)(22).…”

Section: Plasmodium Detection By Elisamentioning

confidence: 99%

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Abundance, composition and natural infection of Anopheles mosquitoes from two malaria-endemic regions of Colombia

et al. 2017

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Introduction:In Colombia there are three Anopheles species implicated in malaria transmission as primary vectors; however, the local role of some Anopheles species must still be defined. Objective: To determine the abundance, composition and natural infection rates for Anopheles mosquitoes with Plasmodium spp. in two malaria-endemic regions of Colombia. Materials and methods: Anopheles mosquitoes were collected using the human-landing catches and while resting in livestock corrals in nine localities of two malaria-endemic regions of Colombia. Mosquitoes were morphologically identified and confirmed by PCR-RFLP-ITS2. Identified mosquitoes were processed and tested for Plasmodium parasite infection by ELISA and ssrRNA-based nested PCR. Results: We collected 1,963 Anopheles mosquitoes corresponding to nine species. The most abundant species were Anopheles nuneztovari (53.5%) and A. darlingi (34.5%), followed by A. triannulatus s.l. (6%), and other species (≈5.9%). Three species were naturally infected with Plasmodium spp.: A. darlingi, A. nuneztovari and A. triannulatus s.l. Conclusions: Natural infection of A. darlingi and A. nuneztovari indicate that these malaria vectors continue to be effective carriers of Plasmodium in the localities under study in Valle del Cauca and Chocó. Additionally, the infected A. triannulatus s.l. collected in livestock corrals in the locality of the department of Córdoba suggests the need for further studies to define the epidemiological importance of this species given its abundance and opportunistic anthropophilic behavior.

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Section: Discussionmentioning

confidence: 83%

“…Localities were selected based on official reports of malaria cases and the geographic distribution of malaria vectors (15,16).…”

Section: Study Areamentioning

confidence: 99%

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Abundance, composition and natural infection of Anopheles mosquitoes from two malaria-endemic regions of Colombia

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“…Moreover, in the malaria-heavy coastal regions of South America and in the eastern slopes of the Venezuelan Andes, members of this subgenus seem to be the most important species involved in the transmission of malaria (Benítez et al 2004, Montoya-Lerma et al 2011. Similarly, the species of Kerteszia have been incriminated in the transmission of "malaria by bromeliads", which is typical of protected areas because the (Carvajal et al 1989, Gutierrez et al 2008 and Anopheles lepidotus (Quiñones et al 1984, Montoya-Lerma et al 2011.In Colombia, seven species of the subgenus have been reported, among which An. neivai and An.…”

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Morphology of the larvae, male genitalia and DNA sequences of Anopheles (Kerteszia) pholidotus (Diptera: Culicidae) from Colombia

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et al. 2014

Mem. Inst. Oswaldo Cruz

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(Marrelli et al. 2007), with some species present at altitudes of more than 1,000 m above sea level (a.s.l.) (Cova-García 1961, Harbach & Navarro 1996.Kerteszia subgenus consists of species that have been incriminated as malaria vectors and are related to the emergence of malaria in thermal floors at altitudes of over 1,500 m a.s.l. Moreover, in the malaria-heavy coastal regions of South America and in the eastern slopes of the Venezuelan Andes, members of this subgenus seem to be the most important species involved in the transmission of malaria (Benítez et al. 2004, Montoya-Lerma et al. 2011. Similarly, the species of Kerteszia have been incriminated in the transmission of "malaria by bromeliads", which is typical of protected areas because the (Carvajal et al. 1989, Gutierrez et al. 2008 and Anopheles lepidotus (Quiñones et al. 1984, Montoya-Lerma et al. 2011.In Colombia, seven species of the subgenus have been reported, among which An. neivai and An. lepidotus are considered malaria vectors, while Anopheles boliviensis is considered a seasonal vector only (Quiñones et al. 1984, Montoya et al. 1994, Olano et al. 2001.Since 1984, An. lepidotus has been considered, using epidemiological evidence (namely, mosquito density correlated with malaria cases; 98% of the Anopheles species found in the malaria focal area corresponded to this species), to be one of the species responsible for malaria transmission in Colombia, particularly in the focal area of Cunday-Villarrica, Department of Tolima. It has been suggested that An. lepidotus is part of a complex of species in which members of this complex are difficult to be differentiated morphologically. Specimens of Kerteszia from Ecuador and Venezuela have been analysed using molecular tools, thereby providing evidence for a complex of species: An. lepidotus could exist with Anopheles pholidotus and An. boliviensis in a complex of at least (2009), based on the analysis of male genitalia and larvae of four specimens from Cunday-Villarrica, suggested the possibility that An. pholidotus occurred in this region. The importance of the above conclusion is that in Colombia, the region of Cunday-Villarrica is considered to be a focal area of malaria, where An. lepidotus had been incriminated as the possible vector for more than 25 years (Quiñones et al. 1984).Recently, Harrison et al. (2012) resolved the problem of separating An. lepidotus from An. pholidotus females by re-describing both species and preparing a taxonomic key. These methods were used to differentiate females, the IV instar larvae, pupae and male genitalia of An. lepidotus from other species of Kerteszia. [1981][1982][1983] in Tolima. Therefore, this conclusion was pending the analysis of the morphology of associated stages, the male genitalia and the inclusion of DNA sequences from samples of An. pholidotus that were recently obtained from the study area.Since the controversy surrounding the identity of the vector in Tolima began more than 30 years ago, the aim of this study was to provide updated and addit...

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“…falciparum in Buenaventura (5). As regards its biology, immature stages develop in bromeliads at mangrove (1).…”

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Phylogenetic signal at the Cytb-SertRNA-IG1-ND1 mitochondrial region in Anopheles (Kerteszia) neivai Howard, Dyar & Knab, 1913

et al. 2017

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Introducción. El ADN mitocondrial ha demostrado su utilidad para el estudio de la evolución en los insectos. Existen algunos genes mitocondriales como el citocromo b (Cytb) y el gen de transferencia para el aminoácido serina (SertRNA) que pueden usarse en el diagnóstico de especies estrechamente relacionadas.Objetivo. Explorar la utilidad filogenética de la región Cytb-SertRNA-IG1-ND1 para detectar posibles especies crípticas en Anopheles neivai.Materiales y métodos. Se recolectaron especímenes en Colombia, Guatemala y en la localidad tipo en Panamá, los cuales se secuenciaron y se compararon mediante el polimorfismo de ADN en toda la región y mediante la simulación de estructuras secundarias del gen SertRNA.Resultados. Se obtuvieron las secuencias de especímenes de A. neivai (34) y A. pholidotus (2).Conclusiones. Se detectaron algunos polimorfismos para la regiónCytb-SertRNA-IG1-ND1 en A. neivai, pero no así especies crípticas.

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Natural infectivity of Anopheles species from the Pacific and Atlantic Regions of Colombia

Cited by 69 publications

References 36 publications

Abundance, composition and natural infection of Anopheles mosquitoes from two malaria-endemic regions of Colombia

Abundance, composition and natural infection of Anopheles mosquitoes from two malaria-endemic regions of Colombia

Morphology of the larvae, male genitalia and DNA sequences of Anopheles (Kerteszia) pholidotus (Diptera: Culicidae) from Colombia

Phylogenetic signal at the Cytb-SertRNA-IG1-ND1 mitochondrial region in Anopheles (Kerteszia) neivai Howard, Dyar & Knab, 1913

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