Natural Cryoprotective and Cytoprotective Agents in Cryopreservation: A Focus on Melatonin

Marcantonini, Giada; Bartolini, Desirée; Zatini, Linda; Costa, Stefania; Passerini, Massimiliano; Rende, Mario; Luca, Giovanni; Basta, Giuseppe; Murdolo, Giuseppe; Calafiore, Riccardo; Galli, Francesco

doi:10.3390/molecules27103254

Cited by 20 publications

(20 citation statements)

References 98 publications

(149 reference statements)

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“…The results of the present study indicated that adding glycine at a concentration of 1.0 mM could alleviate the mechanical and osmotic injury that occurred during the vitrification process (Bojic et al, 2021;Marcantonini et al, 2022;Pegg, 2007). Here in, we found that the post-warming viability was the highest in the group supplemented with 1.0-mM glycine indicating the positive impact of this concentration on the survivability of vitrified/warmed COCs.…”

Section: Discussionsupporting

confidence: 60%

Influences of glycine supplementation during vitrification on the developmental potential of vitrified/warmed immature dromedary camel oocytes

Yaqout

Bard

Elwishy

et al. 2023

Reprod Domestic Animals

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Oocytes experience detrimental osmotic stress during vitrification and warming procedures because of the osmolality imbalance between the vitrification‐warming fluids and the intracellular environment. Cellular osmotic homeostasis can be preserved by glycine, a powerful osmolyte with antioxidant properties. We aimed to examine the influences of supplementing glycine to the vitrification solutions (VS) on the developmental potential of vitrified/warmed immature dromedary camel oocytes following IVM/IVF and in vitro embryo culture (IVC). Cumulus oocyte complexes (COCs) were collected from dromedary camel ovaries and randomly allocated into two groups namely control (oocytes subjected directly to IVM) and vitrified (COCs were vitrified into VS supplemented with 0.0, 0.5, 1.0 or 2.0 mM glycine). For vitrification, COCs were equilibrated for 3 min in 12.5% ethylene glycol; EG plus 12.5% dimethyl sulfoxide; DMS and then they were vitrified for 60 s in VS composed of 25% EG + 25% DMSO using solid surface vitrification (SSV). Warming of vitrified oocytes was conducted in decreasing concentrations of trehalose solution. Following vitrification and warming, the morphologically viable oocytes were subjected to IVM for 36 h. Matured oocytes were then fertilized in vitro by epididymal spermatozoa and cultured for seven days. The results showed that the percentage of viable oocytes assessed by trypan blue stain was significantly higher (p ≤ .05) in the 1.0 mM glycine‐supplemented group than 0.0‐ and 2.0‐mM glycine‐supplemented ones (90.0 % vs. 80.0% and 76.6%, respectively). However, no significant difference was observed between 0.5 mM glycine and other vitrified groups. Nuclear maturation rates, cleavage (48‐h post‐insemination; pi) and blastocyst rate (7‐days pi) were significantly lower in vitrified groups than control ones (p ≤ .05). Among vitrified groups, these parameters were the highest in the 1.0 mM glycine‐supplemented group. Taken together, supplementation of vitrification solutions with 1.0 mM glycine could enhance the developmental potential of vitrified/warmed immature dromedary camel oocytes.

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Section: Discussionsupporting

confidence: 60%

Influences of glycine supplementation during vitrification on the developmental potential of vitrified/warmed immature dromedary camel oocytes

Yaqout

Bard

Elwishy

et al. 2023

Reprod Domestic Animals

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“…At the cellular level, its toxicity depends on different mechanisms, including membrane effects, alterations in cellular permeability, and interactions with cellular proteins and mitochondrial function. Several strategies have been proposed to minimize the toxicity of cryoprotective agents, including the combination of chemical agents with different levels of toxicity with less toxic or neutralizing cryoprotective agents (Marcantonini et al, 2022), as well as the combination of cryoprotective agents and antioxidants to reduce cell damage caused by osmotic dehydration, the formation of large ice crystals and the incidence of reactive oxygen species (ROS) during cooling and rewarming, thus improving the efficiency of cryopreservation (Liu et al, 2021).…”

Section: Resultsmentioning

confidence: 99%

Effects of droplet vitrification on the regeneration of apical shoot tips of mangabeira accessions

Santana

Oliveira

Santos

et al. 2022

RSD

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The mangabeira (Hancornia speciosa Gomes) is a fruit tree of high potential that is distributed in several regions of the Brazil, but which is of great social economic importance for the Northeast region. Due to the wide possibility of using its fruits that can be consumed in natura or industrialized, and that represent a financial source for traditional communities that practice the extractivism of this species. The objective of this study was to evaluate the efficiency of the droplet vitrification technique for the long-term conservation of five mangabeira accessions, as well as to verify if there is any different behavior among the accessions to the possible effects of the cryopreservation process. The apical shoot tips were subjected to different periods (30 and 50 min for test I; 25, 50 and 75 min for test II) of exposure to plant vitrification solution 2 (PVS2) before plunging into liquid nitrogen. To evaluate the effects of cryoprotective solutions and exposed times on apical shoot tips exposed or not exposed to liquid nitrogen, the percentage of regeneration were observed at 30th and 60th day of in vitro cultivation. It was possible to cryopreserve the apical shoot tips from five accessions studied through the droplet vitrification technique. Adjusts must be made in the droplet vitrification technique steps in the pre-culture and post-culture media so that there is a mitigation of the damage caused during the cryopreservation.

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“…Melatonin, a type of indoleamine and free radical scavenger, has been reported to exhibit various antioxidative effects through G-protein-coupled receptors (GPCR). Recently reported studies have focused on the protective effect of melatonin in maintaining fertility in several species of animals and humans [ 25 , 27 , 28 , 29 , 30 , 31 , 34 , 39 , 137 , 138 , 139 ]. Even in humans, melatonin has been reported to be very useful as a sperm cryoprotectant and it has a significant effect on the toxicity and damage recovery of frozen sperm.…”

Section: Biomaterials In Gamete and Embryo Cryopreservationmentioning

confidence: 99%

Application of Nanoparticles and Melatonin for Cryopreservation of Gametes and Embryos

Choi

Jang

2022

CIMB

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Cryopreservation of gametes and embryos, a technique widely applied in human infertility clinics and to preserve desirable genetic traits of livestock, has been developed over 30 years as a component of the artificial insemination process. A number of researchers have conducted studies to reduce cell toxicity during cryopreservation using adjuvants leading to higher gamete and embryo survival rates. Melatonin and Nanoparticles are novel cryoprotectants and recent studies have investigated their properties such as regulating oxidative stresses, lipid peroxidation, and DNA fragmentation in order to protect gametes and embryos during vitrification. This review presented the current status of cryoprotectants and highlights the novel biomaterials such as melatonin and nanoparticles that may improve the survivability of gametes and embryos during this process.

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Natural Cryoprotective and Cytoprotective Agents in Cryopreservation: A Focus on Melatonin

Cited by 20 publications

References 98 publications

Influences of glycine supplementation during vitrification on the developmental potential of vitrified/warmed immature dromedary camel oocytes

Influences of glycine supplementation during vitrification on the developmental potential of vitrified/warmed immature dromedary camel oocytes

Effects of droplet vitrification on the regeneration of apical shoot tips of mangabeira accessions

Application of Nanoparticles and Melatonin for Cryopreservation of Gametes and Embryos

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