Natural compounds from <i>Leea macrophylla</i> enhance phagocytosis and promote osteoblasts differentiation by alkaline phosphatase, type 1 collagen, and osteocalcin gene expression

Raiyan, Sayema; Ma, Rahman; Mamun, Abdullah Al; Asim, Muzammal Haque; Makki, Arwa A.; Hajjar, Dina; Alelwani, Walla; Tangpong, Jitbanjong; Mathew, Bijo

doi:10.1002/jbm.a.37103

Cited by 4 publications

(3 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, this inspection helps to figure out the functional groups that have the inclination to reduce silver ions and wrap up the bio-reduced LM-AgNPs. It was reported that the extract of LM leaves was rich in phenolic and flavonoid contents and the compounds had been spotted in methanol extract of Leea macrophylla by GC–MS [ 45 ].…”

Section: Resultsmentioning

confidence: 99%

Evaluation of antibacterial activity, in-vitro cytotoxicity and catalytic activity of biologically synthesized silver nanoparticles using leaf extracts of Leea macrophylla

Sharmin,

Islam,

Saha

et al. 2023

Heliyon

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Evaluation of antibacterial activity, in-vitro cytotoxicity and catalytic activity of biologically synthesized silver nanoparticles using leaf extracts of Leea macrophylla

Sharmin,

Islam,

Saha

et al. 2023

Heliyon

View full text Add to dashboard Cite

“…The expression of the osteogenic marker gene ALP bolsters concomitantly with the expression of the RUNX2 gene [ 30 , 31 ]. ALP facilitates the synthesis of new collagenous matrix and the release of matrix-bound vesicles to regulate matrix mineralization by the osteoblasts [ 32 ]. Upon matrix encapsulation, the osteoblasts transform into osteocytes.…”

Section: Discussionmentioning

confidence: 99%

Liuwei Dihuang Pills Enhance Osteogenic Differentiation in MC3T3-E1 Cells through the Activation of the Wnt/β-Catenin Signaling Pathway

Zhao,

Liang,

Yang

et al. 2024

Pharmaceuticals

View full text Add to dashboard Cite

Objective: The therapeutic efficacy and molecular mechanisms of traditional Chinese medicines (TCMs), such as Liuwei Dihuang pills (LWDH pills), in treating osteoporosis (OP) remain an area of active research and interest in modern medicine. This study investigated the mechanistic underpinnings of LWDH pills in the treatment of OP based on network pharmacology, bioinformatics, and in vitro experiments. Methods: The active ingredients and targets of LWDH pills were retrieved through the TCMSP database. OP-related targets were identified using the CTD, GeneCards, and DisGeNET databases. The STRING platform was employed to construct a protein–protein interaction (PPI) network, and core targets for LWDH pills in treating OP were identified. The GO functional and KEGG pathway enrichment analyses for potential targets were performed using the R package “clusterProfiler”. A “drug–target” network diagram was created using Cytoscape 3.7.1 software. The viability of MC3T3-E1 cells was evaluated using the CCK-8 method after treatment with various concentrations (1.25%, 2.5%, 5%, and 10%) of LWDH pill-medicated serum for 24, 48, and 72 h. Following a 48 h treatment of MC3T3-E1 cells with LWDH pill-medicated serum, the protein levels of collagen Ⅰ, RUNX2, Wnt3, and β-catenin were quantified using the Western blot analysis, and the activity of alkaline phosphatase (ALP) was measured. Results: A total of 197 putative targets for LWDH pills for OP treatment were pinpointed, from which 20 core targets were singled out, including TP53, JUN, TNF, CTNNB1 (β-catenin), and GSK3B. The putative targets were predominantly involved in signaling pathways such as the Wnt signaling pathway, the MAPK signaling pathway, and the PI3K-Akt signaling pathway. The intervention with LWDH pill-medicated serum for 24, 48, and 72 h did not result in any notable alterations in the cell viability of MC3T3-E1 cells relative to the control group (all p > 0.05). Significant upregulation in protein levels of collagen Ⅰ, RUNX2, Wnt3, and β-catenin in MC3T3-E1 cells was observed in response to the treatment with 2.5%, 5%, and 10% of LWDH pill-medicated serum in comparison to that with the 10% rabbit serum group (all p < 0.05). Furthermore, the intervention with LWDH pill-medicated serum resulted in the formation of red calcified nodules in MC3T3-E1 cells, as indicated by ARS staining. Conclusions: LWDH pills may upregulate the Wnt/β-catenin signaling pathway to elevate the expression of osteogenic differentiation proteins, including collagen Ⅰ and RUNX2, and to increase the ALP activity in MC3T3-E1 cells for the treatment of OP.

show abstract

“…ALP activity was used to characterize autophagy levels [ 37 ]. In Saccharomyces cerevisiae , the precursor of ALP in cytoplasm is encapsulated in autophagosomes and then transported to vacuoles, in which it can be degraded by enzymes.…”

Section: Discussionmentioning

confidence: 99%

Autophagy Alters the Susceptibility of Candida albicans Biofilms to Antifungal Agents

Shen

Duan

et al. 2023

Microorganisms

View full text Add to dashboard Cite

Candida albicans (C. albicans) reigns as a major cause of clinical candidiasis. C. albicans biofilms are known to increase resistance to antifungal agents, making biofilm-related infections particularly challenging to treat. Drug resistance is of particular concern due to the spread of multidrug-resistant fungal pathogens, while autophagy is crucial for the maintenance of cellular homeostasis. Therefore, this study aimed to investigate the effects of an activator and an inhibitor of autophagy on the susceptibility of C. albicans biofilms to antifungal agents and the related mechanisms. The susceptibility of C. albicans biofilms to different antifungal agents after treatment with or without the autophagy activator or inhibitor was evaluated using XTT assay. Alkaline phosphatase (ALP) activity and reactive oxygen species (ROS) level, as well as the expression of ROS-related and autophagy-related genes, were examined to evaluate the autophagic activity of C. albicans biofilms when treated with antifungal agents. The autophagosomes were observed by transmission electron microscopy (TEM). The susceptibility of C. albicans biofilms to antifungal agents changed when autophagy changed. The ALP activity and ROS level of C. albicans biofilms increased with the treatment of antifungal agents, and autophagosomes could be observed in C. albicans biofilms. Autophagy was involved in the susceptibility of C. albicans biofilms to antifungal agents.

show abstract

Natural compounds from Leea macrophylla enhance phagocytosis and promote osteoblasts differentiation by alkaline phosphatase, type 1 collagen, and osteocalcin gene expression

Cited by 4 publications

References 40 publications

Evaluation of antibacterial activity, in-vitro cytotoxicity and catalytic activity of biologically synthesized silver nanoparticles using leaf extracts of Leea macrophylla

Evaluation of antibacterial activity, in-vitro cytotoxicity and catalytic activity of biologically synthesized silver nanoparticles using leaf extracts of Leea macrophylla

Liuwei Dihuang Pills Enhance Osteogenic Differentiation in MC3T3-E1 Cells through the Activation of the Wnt/β-Catenin Signaling Pathway

Autophagy Alters the Susceptibility of Candida albicans Biofilms to Antifungal Agents

Contact Info

Product

Resources

About