Naringin Attenuates High Fat Diet Induced Non-alcoholic Fatty Liver Disease and Gut Bacterial Dysbiosis in Mice

Mu, Hongna; Zhou, Qi; Yang, Ruiyue; Zeng, Jie; Li, Xianghui; Zhang, Ranran; Tang, Weiqing; Li, Hongxia; Wang, Siming; Shen, Tao; Huang, Xiuqing; Dou, Lin; Dong, Jun

doi:10.3389/fmicb.2020.585066

Cited by 123 publications

(95 citation statements)

References 70 publications

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“…In order to explore the possible components of CRP extract, UPLC-Q/TOF MS analysis was first performed. The results showed that among the seven components detected, naringin, hesperidin, poncirin, nobiletin and tangeretin all exhibited an improvement effect on glycolipid metabolism disorder (19)(20)(21)(22)(23), while naringin, hesperidin, nobiletin and tangeretin have been found to regulate the gut microbiome (19,(23)(24)(25). It is worth noting that hesperidin is a component that should be detected in the drug standard of CRP extract, suggesting that hesperidin may play a key role in this process (Supplementary Materials: Drug standard of CRP extract).…”

Section: Discussionmentioning

confidence: 99%

Citrus reticulatae pericarpium Extract Decreases the Susceptibility to HFD-Induced Glycolipid Metabolism Disorder in Mice Exposed to Azithromycin in Early Life

You

Zhou

et al. 2021

Front. Immunol.

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BackgroundStudies have shown that gut microbe disorder in mice due to early-life antibiotic exposure promotes glycolipid metabolism disorder in adulthood. However, the underlying mechanism remains unclear and there is not yet an effective intervention or treatment for this process.PurposeThe study investigated whether early-life azithromycin (AZT) exposure in mice could promote high-fat diet (HFD)-induced glycolipid metabolism disorder in adulthood. Moreover, the effect of citrus reticulata pericarpium (CRP) extract on glycolipid metabolism disorder via regulation of gut microbiome in mice exposed to antibodies early in life were investigated.Methods and ResultsThree-week-old mice were treated with AZT (50 mg/kg/day) via drinking water for two weeks and then were fed a CRP diet (1% CRP extract) for four weeks and an HFD for five weeks. The results showed that early-life AZT exposure promoted HFD-induced glycolipid metabolism disorder, increased the levels of inflammatory factors, promoted the flora metabolism product trimethylamine N-oxide (TMAO), and induced microbial disorder in adult mice. Importantly, CRP extract mitigated these effects.ConclusionTaken together, these findings suggest that early-life AZT exposure increases the susceptibility to HFD-induced glycolipid metabolism disorder in adult mice, and CRP extract can decrease this susceptibility by regulating gut microbiome.

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Section: Discussionmentioning

confidence: 99%

Citrus reticulatae pericarpium Extract Decreases the Susceptibility to HFD-Induced Glycolipid Metabolism Disorder in Mice Exposed to Azithromycin in Early Life

You

Zhou

et al. 2021

Front. Immunol.

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“…These results showed that B. uniformis CBA7346 administration has a protective effect against liver injury. NAFLD promotes liver inflammation; NAFLD patients therefore have disrupted gut barrier integrity and bacterial overgrowth, which can result in the release of lipopolysaccharide (LPS) through the gut-liver axis, leading to hepatocyte death and lipid accumulation [ 25 ]. Mice on an HFD, when treated with B. uniformis CBA7346, showed decreased LPS release in the serum confirming that B. uniformis CBA7346 also prevented the passage of LPS into the blood.…”

Section: Discussionmentioning

confidence: 99%

“…SREBP1 activated by insulin, and ChREBP activated by glucose regulates hepatic lipogenesis [ 6 ]. SREBP1 and ChREBP are central regulators of most hepatic lipid synthesis-related genes, including the ACC, FAS, and SCD1 [ 25 , 28 ]. In the HU group which is on an HFD, the expression of hepatic lipogenesis-related proteins such as the SREBP1, ChREBP, ACC, and FAS was significantly downregulated, but not that of SCD1.…”

Section: Discussionmentioning

confidence: 99%

Amelioration of Hepatic Steatosis in Mice through Bacteroides uniformis CBA7346-Mediated Regulation of High-Fat Diet-Induced Insulin Resistance and Lipogenesis

Lee

Jhun

et al. 2021

Nutrients

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Dietary habits and gut microbiota play an essential role in non-alcoholic fatty liver disease (NAFLD) and related factors such as insulin resistance and de novo lipogenesis. In this study, we investigated the protective effects of Bacteroides uniformis CBA7346, isolated from the gut of healthy Koreans, on mice with high-fat diet (HFD)-induced NAFLD. Administration of B. uniformis CBA7346 reduced body and liver weight gain, serum alanine aminotransferase and aspartate aminotransferase levels, liver steatosis, and liver triglyceride levels in mice on an HFD; the strain also decreased homeostatic model assessment for insulin resistance values, as well as serum cholesterol, triglyceride, lipopolysaccharide, leptin, and adiponectin levels in mice on an HFD. Moreover, B. uniformis CBA7346 controlled fatty liver disease by attenuating steatosis and inflammation and regulating de novo lipogenesis-related proteins in mice on an HFD. Taken together, these findings suggest that B. uniformis CBA7346 ameliorates HFD-induced NAFLD by reducing insulin resistance and regulating de novo lipogenesis in obese mice.

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“…The modulation of the gut microbial profile by PREP disruption may prevent NAFLD by altering the relative abundance of several “beneficial indicators” in the cecum, thereby promoting homeostasis. Odoribacter and Oscillibacter are closely associated with intestinal epithelial homeostasis, while Lachnospiraceae NK4A139 negatively correlated with serum lipid levels ( Zhao et al, 2019 ; Mu et al, 2020 ). Besides, Ruminiclostridium 9 belongs to the Ruminococcaceae family; reportedly, a high abundance of Ruminococcaceae in the cecum could effectively prevent malnutrition ( Million et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Prolyl Endopeptidase Gene Disruption Improves Gut Dysbiosis and Non-alcoholic Fatty Liver Disease in Mice Induced by a High-Fat Diet

Lin

Chen

Fan

2021

Front. Cell Dev. Biol.

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The gut-liver axis is increasingly recognized as being involved in the pathogenesis and progression of non-alcoholic fatty liver disease (NAFLD). Prolyl endopeptidase (PREP) plays a role in gut metabolic homeostasis and neurodegenerative diseases. We investigated the role of PREP disruption in the crosstalk between gut flora and hepatic steatosis or inflammation in mice with NAFLD. Wild-type mice (WT) and PREP gene knocked mice (PREPgt) were fed a low-fat diet (LFD) or high-fat diet (HFD) for 16 or 24 weeks. Murine gut microbiota profiles were generated at 16 or 24 weeks. Liver lipogenesis-associated molecules and their upstream mediators, AMP-activated protein kinase (AMPK) and sirtuin1 (SIRT1), were detected using RT-PCR or western blot in all mice. Inflammatory triggers and mediators from the gut or infiltrated inflammatory cells and signal mediators, such as p-ERK and p-p65, were determined. We found that PREP disruption modulated microbiota composition and altered the abundance of several beneficial bacteria such as the butyrate-producing bacteria in mice fed a HFD for 16 or 24 weeks. The level of butyrate in HFD-PREPgt mice significantly increased compared with that of the HFD-WT mice at 16 weeks. Interestingly, PREP disruption inhibited p-ERK and p-p65 and reduced the levels of proinflammatory cytokines in response to endotoxin and proline-glycine-proline, which guided macrophage/neutrophil infiltration in mice fed a HFD for 24 weeks. However, at 16 weeks, PREP disruption, other than regulating hepatic inflammation, displayed improved liver lipogenesis and AMPK/SIRT1 signaling. PREP disruption may target multiple hepatic mechanisms related to the liver, gut, and microbiota, displaying a dynamic role in hepatic steatosis and inflammation during NAFLD. PREP might serve as a therapeutic target for NAFLD.

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Naringin Attenuates High Fat Diet Induced Non-alcoholic Fatty Liver Disease and Gut Bacterial Dysbiosis in Mice

Cited by 123 publications

References 70 publications

Citrus reticulatae pericarpium Extract Decreases the Susceptibility to HFD-Induced Glycolipid Metabolism Disorder in Mice Exposed to Azithromycin in Early Life

Citrus reticulatae pericarpium Extract Decreases the Susceptibility to HFD-Induced Glycolipid Metabolism Disorder in Mice Exposed to Azithromycin in Early Life

Amelioration of Hepatic Steatosis in Mice through Bacteroides uniformis CBA7346-Mediated Regulation of High-Fat Diet-Induced Insulin Resistance and Lipogenesis

Prolyl Endopeptidase Gene Disruption Improves Gut Dysbiosis and Non-alcoholic Fatty Liver Disease in Mice Induced by a High-Fat Diet

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