Nanozymes: Gold‐Nanoparticle‐Based Transphosphorylation Catalysts

Manea, Flavio; Houillon, Florence Bodar; Pasquato, Lucia; Scrimin, Paolo

doi:10.1002/anie.200460649

Cited by 520 publications

(329 citation statements)

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“…1,2 An early example was reported by Manea et al; they used gold nanoparticles (AuNPs) to cleave phosphodiester bonds, mimicking the nuclease activity. 3 Recently, AuNPs were employed to mimic glucose oxidase, where glucose was oxidized to produce hydrogen peroxide. [4][5][6] Esterase activity was also achieved with β-cyclodextrin-modified AuNPs.…”

mentioning

confidence: 99%

Attaching DNA to Nanoceria: Regulating Oxidase Activity and Fluorescence Quenching

Pautler

Kelly

Huang

et al. 2013

ACS Appl. Mater. Interfaces

194

175

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Abstract. Cerium oxide nanoparticles (nanoceria) have recently emerged as a nanozyme with oxidase activity. In this work we present a few important interfacial properties of nanoceria. First, the surface charge of nanoceria can be controlled not only by adjusting pH but also by adsorption of simple inorganic anions. Adsorption of phosphate and citrate gives negatively charged surface over a broad pH range. Second, nanoceria adsorbs DNA via the DNA phosphate backbone in a sequence-independent manner; DNA adsorption inhibits its oxidase activity. Other anionic polymers display much weaker inhibition effects. Adsorption of simple inorganic phosphate does not have the inhibition effect. Third, nanoceria is a quencher for many fluorophores. These discoveries provide an important understanding for further use of nanoceria in biosensor development, materials science and nanotechnology.Keywords: cerium oxide, nanozymes, adsorption, surface charge, oxidaseEnzymes usually refer to protein-based biocatalysts with high activity and substrate specificity; they play crucial roles in life and are the central molecules in biochemistry, biotechnology and analytical

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mentioning

confidence: 99%

Attaching DNA to Nanoceria: Regulating Oxidase Activity and Fluorescence Quenching

Pautler

Kelly

Huang

et al. 2013

ACS Appl. Mater. Interfaces

194

175

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“…The use of gold nanoparticles as enzyme mimics, also called nanozymes, [35] has been recently developed (see Pasquato et al [36] for a review). The fundamental idea is to engineer a A C H T U N G T R E N N U N G microenvironment, within a self-assembled monolayer, that A C H T U N G T R E N N U N G resembles the catalytic site of natural enzymes.…”

Section: Artificial Enzymesmentioning

confidence: 99%

Peptide‐Capped Gold Nanoparticles: Towards Artificial Proteins

Lévy

2006

ChemBioChem

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Peptides can be designed to form self-assembled monolayers on gold nanoparticles to give nanomaterials with some chemical properties analogous to those of proteins. A variety of molecular-recognition properties are readily integrated within the peptide monolayer. Monofunctionalized nanoparticles are obtained by using separation methods that have been optimized for proteins. Recent applications as artificial enzymes and artificial enzyme substrates are presented. The limitations and long-term potential of peptide-capped nanoparticles as artificial proteins are discussed.

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“…These materials are characterized by the properties of the organic molecules that constitute the monolayer but also by the properties of the metal cluster core. Multivalency [105], which is at the basis of the amplification of the binding events with functional nanoparticles, has been exploited for the preparation of catalytic systems in which a cluster of functional groups led to catalytic activity which was not just the sum of the individual contributions [106]. We prepared a thiol-functionalized dipeptide by N-acylation of HisPhe speculating that, in this system, the free carboxylate/carboxylic acid of phenylalanine could constitute a simple function for catalysis providing, in conjunction with the imidazole of histidine, either base/nucleophilic and/or acid catalytic contributions.…”

Section: Nanoparticle-and Dendrimer-based Functional (Pseudo)peptidesmentioning

confidence: 99%