Nanotechnology and Plant Tissue Culture

Álvarez, Sandra Pérez; Magallanes-Tapia, Marco Antonio; Vega, María Esther González; Ardisana, Eduardo Fidel Héctor; Medina, J.; Zamora, Gabriela Lizbeth Flores; Bustamante, Daniela Valenzuela

doi:10.1007/978-3-030-12496-0_12

Cited by 11 publications

(9 citation statements)

References 162 publications

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“…The sterile environment and the chemically defined culture medium composition avoid most of the extraneous factors that could influence nanoparticle interaction with plants. The positive aspects of different ENMs applications in plant in vitro cultures have been reported and are most often for the elimination of microbial contamination, the induction of caulogenesis, adventitious organogenesis, somatic embryogenesis, genetic transformation, metabolite production [ 9 ], and for improving the efficiency of cryopreservation protocols [ 10 ]. Nevertheless, nanomaterials may also negatively influence plant metabolism, growth, and development.…”

Section: Introductionmentioning

confidence: 99%

Silver Nanoparticles Effects on In Vitro Germination, Growth, and Biochemical Activity of Tomato, Radish, and Kale Seedlings

Tymoszuk

2021

Materials

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The interactions between nanoparticles and plant cells are still not sufficiently understood, and studies related to this subject are of scientific and practical importance. Silver nanoparticles (AgNPs) are one of the most commonly produced and used nanomaterials. This study aimed to investigate the influence of AgNPs applied at the concentrations of 0, 50, and 100 mg·L−1 during the process of in vitro germination as well as the biometric and biochemical parameters of developed seedlings in three vegetable species: Solanum lycopersicum L. ‘Poranek’, Raphanus sativus L. var. sativus ‘Ramona’, and Brassica oleracea var. sabellica ‘Nero di Toscana’. The application of AgNPs did not affect the germination efficiency; however, diverse results were reported for the growth and biochemical activity of the seedlings, depending on the species tested and the AgNPs concentration. Tomato seedlings treated with nanoparticles, particularly at 100 mg·L−1, had shorter shoots with lower fresh and dry weights and produced roots with lower fresh weight. Simultaneously, at the biochemical level, a decrease in the content of chlorophylls and carotenoids and an increase in the anthocyanins content and guaiacol peroxidase (GPOX) activity were reported. AgNPs-treated radish plants had shorter shoots of higher fresh and dry weight and longer roots with lower fresh weight. Treatment with 50 mg·L−1 and 100 mg·L−1 resulted in the highest and lowest accumulation of chlorophylls and carotenoids in the leaves, respectively; however, seedlings treated with 100 mg·L−1 produced less anthocyanins and polyphenols and exhibited lower GPOX activity. In kale, AgNPs-derived seedlings had a lower content of chlorophylls, carotenoids, and anthocyanins but higher GPOX activity of and were characterized by higher fresh and dry shoot weights and higher heterogeneous biometric parameters of the roots. The results of these experiments may be of great significance for broadening the scope of knowledge on the influence of AgNPs on plant cells and the micropropagation of the vegetable species. Future studies should be aimed at testing lower or even higher concentrations of AgNPs and other NPs and to evaluate the genetic stability of NPs-treated vegetable crops and their yielding efficiency.

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Section: Introductionmentioning

confidence: 99%

Silver Nanoparticles Effects on In Vitro Germination, Growth, and Biochemical Activity of Tomato, Radish, and Kale Seedlings

Tymoszuk

2021

Materials

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“…Similarly, positive and enhancing effect of naphthalene acetic acid (NAA) and BA combinations on shoot multiplication rate was recorded by many researchers such as Somashekar et al (2008) Selenium nanoparticles have wide applications in the fields of medicine, microelectronic, agriculture and animal husbandry, because of its biological and industrial properties. In recent years, there has been an increasing amount of literature on nanotechnology and its applications in the field of plant tissue culture (Álvarez et al, 2019;Abdel-Wahab et al, 2020). It has been noted that callus production and in vitro rooting of tobacco could be stimulated by high concentrations of selenium nanoparticles (265-532µM), where recent evidence suggests that selenium nanoparticles can improve the growth, morphogenesis of tobacco and inhibit the vitrification of tobacco plantlets without any change in the chlorophyll content (Shoeibi et al, 2017).…”

Section: Shootlet Lengthmentioning

confidence: 99%

A New Aspect for In vitro Propagation of Jerusalem Artichoke and Molecular Assessment Using RAPD, ISSR and SCoT Marker Techniques

et al. 2020

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“…Plant in vitro culture is considered a biotechnological process that involved growing plant cells, tissues, or organs in an artificial nutrition media in a controlled environment and aseptic condition. Application of plant in vitro culture for plant propagation and crop improvement has been discussed [1][2][3][4][5]. In addition, it is also utilized for pharmacological purposes [5,6].…”

Section: Introductionmentioning

confidence: 99%

“…Application of plant in vitro culture for plant propagation and crop improvement has been discussed [1][2][3][4][5]. In addition, it is also utilized for pharmacological purposes [5,6]. As far as agriculture is concerned, cell propagation in vitro, has been widely used for massive production of low-proliferating crops, for improvement of crop varieties, and preparation of pathogen-free plants, as well as for maintaining stocks of endangered species as an ex-situ conservation approach [5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, it is also utilized for pharmacological purposes [5,6]. As far as agriculture is concerned, cell propagation in vitro, has been widely used for massive production of low-proliferating crops, for improvement of crop varieties, and preparation of pathogen-free plants, as well as for maintaining stocks of endangered species as an ex-situ conservation approach [5][6][7][8][9][10]. Plant cell culture can be applied to a wide range of botanical species through different methods and procedures and this assumes strategic importance considering the present concern about environmental issues [10].…”

Section: Introductionmentioning

confidence: 99%

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Nanotechnology in Plant Metabolite Improvement and in Animal Welfare

et al. 2022

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Plant tissue culture plays an important role in plant biotechnology due to its potential for massive production of improved crop varieties and high yield of important secondary metabolites. Several efforts have been made to ameliorate the effectiveness and production of plant tissue culture, using biotic and abiotic factors. Nowadays, the addition of nanoparticles as elicitors has, for instance, gained worldwide interest because of its success in microbial decontamination and enhancement of secondary metabolites. Nanoparticles are entities in the nanometric dimension range: they possess unique physicochemical properties. Among all nanoparticles, silver-nanoparticles (AgNPs) are well-known for their antimicrobial and hormetic effects, which in appropriate doses, led to the improvement of plant biomass as well as secondary metabolite accumulation. This review is focused on the evaluation of the integration of nanotechnology with plant tissue culture. The highlight is especially conveyed on secondary metabolite enhancement, effects on plant growth and biomass accumulation as well as their possible mechanism of action. In addition, some perspectives of the use of nanomaterials as potential therapeutic agents are also discussed. Thus, the information provided will be a good tool for future research in plant improvement and the large-scale production of important secondary metabolites. Elicitation of silver-nanoparticles, as well as nanomaterials, function as therapeutic agents for animal well-being is expected to play a major role in the process. However, nanosized supramolecular aggregates have received an increased resonance also in other fields of application such as animal welfare. Therefore, the concluding section of this contribution is dedicated to the description and possible potential and usage of different nanoparticles that have been the object of work and expertise also in our laboratories.

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Nanotechnology and Plant Tissue Culture

Cited by 11 publications

References 162 publications

Silver Nanoparticles Effects on In Vitro Germination, Growth, and Biochemical Activity of Tomato, Radish, and Kale Seedlings

Silver Nanoparticles Effects on In Vitro Germination, Growth, and Biochemical Activity of Tomato, Radish, and Kale Seedlings

A New Aspect for In vitro Propagation of Jerusalem Artichoke and Molecular Assessment Using RAPD, ISSR and SCoT Marker Techniques

Nanotechnology in Plant Metabolite Improvement and in Animal Welfare

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