Nanosulfur: A Potent Fungicide Against Food Pathogen, Aspergillus niger

Choudhury, Samrat Roy; Nair, Kishore Kumar; Kumar, Rajesh; Gogoi, Robin; Srivastava, Chitra; Gopal, Madhuban; Subhramanyam, B. S.; Devakumar, C.; Goswami, Arunava

doi:10.1063/1.3504287

Cited by 60 publications

(31 citation statements)

References 3 publications

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“…An eco-friendly fungicide is under development that uses nanomaterials to release its pathogen killing properties only when it is inside the targeted plant pathogens (Choudhury et al 2010;Abd-Elsalam 2012;Rai and Ingle 2012). Eugenol has antimicrobial activity against a variety a Relative contribution (RC) to variation in wilt incidence calculated as percentage of sum of square of the explained (model) variation Fig.…”

Section: Discussionmentioning

confidence: 99%

Eugenol oil nanoemulsion: antifungal activity against Fusarium oxysporum f. sp. vasinfectum and phytotoxicity on cottonseeds

2015

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The current research deals with the formulation and characterization of bio-based oil-in-water nanoemulsion. The formulated eugenol oil nanoemulsion was characterized by dynamic light scattering, stability test, transmission electron microscopy and thin layer chromatography. The nanoemulsion droplets were found to have a Z-average diameter of 80 nm and TEM study reveals the spherical shape of eugenol oil nanoemulsion (EON). The size of the nanoemulsion was found to be physically stable up to more than 1-month when it was kept at room temperature (25°C). The TEM micrograph showed that the EON was spherical in shape and moderately mono or di-dispersed and was in the range of 50-110 nm. Three concentrations of the nanoformulation were used to evalute the anti-fusarium activity both in vitro and in vivo experiments. SDS-PAGE results of total protein from the Fusarium oxysporum f. sp. vasinfectum (FOV) isolate before and after treatment with eugenol oil nanoemulsion indicate that the content of extra cellular soluble small molecular proteins decreased significantly in EON-treated fungus. Light micrographs of mycelia and spores treated with EON showed the disruption of the fungal structures. The analysis of variance (ANOVA) for Fusarium wilt incidence indicated highly significant (p = 0.000) effects of concentration, genotype, and their interaction. The difference in wilt incidence between concentrations and control was not the same for each genotype, that is, the genotypes responded differently to concentrations. Effects of three EON concentration on germination percentage, and radicle length, were determined in the laboratory. One very interesting finding in the current study is that cotton genotypes was the most important factors in determining wilt incidence as it accounted for 93.18 % of the explained (model) variation. In vitro experiments were conducted to evaluate the potential phytotoxic effect of three EON concentrations. Concentration, genotype and concentration x genotype interaction were all highly significant sources of variation in seed germination; however, interaction was the first in importance as a source of variation followed by the concentration, while genotype was the least important source of variation. These results suggest the potential use of eugenol oil nanoemulsion for protecting seedcotton from Fusarium wilt infection.

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Section: Discussionmentioning

confidence: 99%

Eugenol oil nanoemulsion: antifungal activity against Fusarium oxysporum f. sp. vasinfectum and phytotoxicity on cottonseeds

2015

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“…The lipophilic nature of elemental sulfur (ES) and their natural ability to be accumulated in the epicuticular wax of diverse plant families is already known [14]. In our previous studies, we have already showed that surface reactive SNPs can effectively inhibit the fungal and bacterial growth irrespective of their susceptibilities to ES [22,23,25]. Moreover, it was found that SNPs can effectively inhibit frequency of spore germination in fungi and reduce the content of phospholipids with significant morphological deformities at their surface [22].…”

Section: Introductionmentioning

confidence: 95%

Inhibitory Effects of Sulfur Nanoparticles on Membrane Lipids of Aspergillus niger: A Novel Route of Fungistasis

2012

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Orthorhombic (spherical; ~10 nm) and monoclinic (cylindrical; ~50 nm) sulfur nanoparticles (SNPs) were synthesized and examined for their effects on the total lipid content and desaturase enzymes of Aspergillus niger. Synthesized SNPs were characterized for size with transmission electron microscopy, elemental composition with energy dispersive X-ray spectroscopy and allotropic nature with X-ray diffraction pattern. Both the SNPs considerably reduced total lipid content of the treated fungal isolates with significant down regulation of the expression of various desaturase enzymes (linoleoyl-CoA desaturase, stearoyl-CoA 9-desaturase and phosphatidylcholine desaturase). Unusual high accumulation of saturated fatty acids with depleted lipid layer can be inferred as one of the major reasons of SNPs mediated fungistasis.

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“…minimize the use of fungicides and pesticides in future. Studies have confirmed that metal nanoparticles are effective against plant pathogens, insects and pests (Choudhury et al, 2010). For example, eco-friendly fungicides with nanopesticides are being developed to utilize its implication its pathogen-killing properties only when it is inside the targeted pathogen (Liu, 2006).…”

Section: Issn: 2319-7706 Volume 6 Number 6 (2017) Pp 2821-2830mentioning

confidence: 99%