Nanostructured Polymer Thin Films Fabricated with Brush-based Layer-by-Layer Self-assembly for Site-selective Construction and Drug release

Abstract

Layer-by-Layer (LbL) self-assembly has been investigated for several decades. However, the conventional LbL method has performance problems on the chair-side caused by its cumbersome and time-consuming process. Thus, we investigate a new LbL self-assembly technique for the fast and high efficient preparation process based on the brush. The multilayer films fabricated by simple sequential brushing of polyelectrolyte solutions are compared to the classical dipping method. We characterize the multilayer films by characteristics such as their morphology and thickness, and compare them against those of the classic method by profilometry, atomic force microscopy. We prepare multilayer films with biocompatible polyelectrolytes, chitosan, and alginate incorporated with a hydrophobic drug carrier. For the drug carrier, a poly(ethylene glycol)-block-poly(ε-caprolactone) (PEG-b-PCL) block copolymer is introduced to construct micelles containing dexamethasone, which is a well-known osteogenesis-inducing drug. The hydrogen bonding behavior between adjacent layers and micelles is investigated by Fourier transform infrared spectroscopy. Additionally, we analyze the release profiles, degradation profiles and toxicity of the multilayer films for biomedical applications. From these results, we can identify the brushing LbL method as a reliable and more efficient multilayer film-construction process compared to conventional dipping LbL, especially for practical applications in dental and clinical situations.

Polysaccharide Thin Solid Films for Analgesic Drug Delivery and Growth of Human Skin Cells

“…They also hold a huge scientific interest for several solid-liquid interface studies, especially for the adsorptive interaction of nanoparticles (Taajamaa et al, 2013), polyelectrolytes (Mohan et al, 2015, 2017), biomolecules (Kargl et al, 2015), etc. While significant efforts have been made to formulate drug delivery platforms from organo-soluble synthetic polymers (Vendra et al, 2011; Ponnusamy et al, 2012), the use of natural polymers (e.g., polysaccharides) for such applications is limited and have recently gained a paramount of interest (Zelikin, 2010; Park K. et al, 2018; Park S. et al, 2018). Due to their high compatibility, bio-inertness, diverse physicochemical properties and ease of handling during manufacturing, polysaccharide thin films are excellent candidates for skin-related applications, where a controlled and targeted delivery of pain-relieving analgesic drugs are prerequisite (Miao et al, 2018).…”

“…Until now, the layer-by-layer (LbL) self-assembly technique has been largely used to create nanoscale multi-layered systems, which are of interest for targeted drug delivery applications (Stana et al, 2017; Park K. et al, 2018), where the simplicity and versatility of the preparation procedure lower the overall drug delivery system costs. In general, the LbL technique exploits the alternating deposition of oppositely charged substances (e.g., polyelectrolytes, polypeptides, etc.…”

Tunable Self-Assembled Nanostructures of Electroactive PEGylated Tetra(Aniline) Based ABA Triblock Structures in Aqueous Medium

“…Self-assembly is the simplest approach toward the production of highly ordered functional assemblies of molecules in a wide range of interesting morphologies (e.g., various micellar structures, nanorods, nanoribbons, nanofibers, amongst others) (Gröschel and Müller, 2015; Townsend et al, 2018). Such structures have potential applications in the field of nanotechnology, biomedicine, tissue engineering and drug delivery (Kutikov and Song, 2015; Li et al, 2017; Zhou et al, 2017; Park et al, 2018; Liua et al, 2019). This self-assembly approach produces such intricate morphologies through various secondary interactions (Molla and Ghosh, 2014), including π-π stacking (Herrikhuyzen et al, 2006), hydrogen bonding (Valkama et al, 2006), ionic interactions (Faul, 2014) and hydrophobic interactions (Lombardo et al, 2015).…”

Developing regulatory property of gelatin-tannic acid multilayer films for coating-based nitric oxide gas delivery system

“…We prepared Gel solution and TA solution at a concentration of 1 mg/mL, which is enough for thermodynamically driven film fabrication of polyelectrolytes 50 . To dissolve the Gel, we heated the Gel solution for 30 min at 60 °C after mixing the Gel powder in deionized water.…”