Nanoscaled Biodegradable Metal–Polymeric Three-Dimensional Framework for Endothelial Cell Patterning and Sustained Angiogenesis

Abstract

The current work describes the development of a nanoscaled biodegradable metal polymeric three-dimensional framework with controlled nanotherapeutic release for endothelial cell patterning and sustained angiogenesis for biomedical applications. Biocompatible polymers gelatin and PLGA were used as polymeric nanofibrous three-dimensional framework in a core–shell manner with the gelatin core containing a biodegradable and bioactive metal nanoframework of cobalt caged with PEGylated curcumin by coaxial electrospinning. FTIR results confirmed the presence of nanobioactives in the core region of a coaxial nanofiber. Scanning electron microscopic analysis of the coaxial nanofibrous system showed a three-dimensional architecture that favored endothelial cell adhesion, patterning, migration, and proliferation. The as-synthesized nanoscaled biodegradable metal polymeric three-dimensional core–shell nanofibers exhibited potent antibacterial efficacy. Further, it improved the endothelial cell patterning promoting angiogenesis. The high therapeutic potential of cobalt nanoframework in the nanofibers enhanced the production of vascular endothelial growth factor promoting angiogenesis that resulted in the earlier restoration of wounded tissue compared with untreated control in vivo animal models. The study opens up a new horizon in exploring biodegradable biosorbable metal nanoframework for biomaterial applications. Additionally, the present study opens up a new strategy in developing biodegradable biosorbable biomaterial with enhanced vascularization efficacy to the biomaterial, which is important for acceptance of these biomaterials into the host tissue.

Curcumin‐based nanoformulations alleviate wounds and related disorders: A comprehensive review

“…Coreshell nanofibrous 3D framework using gelatin (as a core) and PLGA (as a shell) was prepared while the gelatin core containing cobalt NPs caged with PEGylated CUR (CUR/Co@GEL/PLGA). 33 Well-established cell morphology was observed on CUR/Co@GEL/PLGA nanofibers compared to GEL/PLGA nanofibers. Establishing longdistance communication between the messenger molecules assists in the enhancement of the cell-cell connection, thereby initiating sprout formation, cell migration, tube formation, and vascularization.…”

“…The 3D porous architecture increases the mechanical strength and fluid retention in the wound site so that the material can easily draw nutrients for the organization of cell adhesion and differentiation. Core–shell nanofibrous 3D framework using gelatin (as a core) and PLGA (as a shell) was prepared while the gelatin core containing cobalt NPs caged with PEGylated CUR (CUR/Co@GEL/PLGA) 33 . Well‐established cell morphology was observed on CUR/Co@GEL/PLGA nanofibers compared to GEL/PLGA nanofibers.…”

Antibacterial Vitamin K3 Carnosine Peptide-Laden Silk Fibroin Electrospun Fibers for Improvement of Skin Wound Healing in Diabetic Rats

“…54 The cumulative release percentage of VKC was determined (by a Perkin-Elmer UV spectrophotometer at 450 nm) and calculated with the standard plot. 60 2.12. Cell Viability Evaluation of Vitamin K3-Carnosine Peptide (VKC).…”

“…Furthermore, 5 mg of the square-shaped prepared SF-VKC mats was allowed to incubate in the presence of 5 mL of fresh PBS at 37 °C with mild shaking for 72 h. PBS (1 mL) was taken from 5 mL of the release medium at different time intervals, and 1 mL of freshly prepared PBS was replaced instead of the withdrawn solution . The cumulative release percentage of VKC was determined (by a Perkin-Elmer UV spectrophotometer at 450 nm) and calculated with the standard plot …”

Pro-angiogenic approach for skeletal muscle regeneration

“…In the pro-angiogenic therapy, biomaterials induce the angiogenesis process and may be set up to deliver pro-angiogenic factors such as MSCs, exosomes, growth factors, and angiomiRs. There are several classes of biomaterials including ceramics [261], composites [262], metallic [263], and polymers [264]. The association of biological factors with bioactive biomaterials (smart biomaterials) are key strategies optimizing pro-angiogenic therapy with fewer adverse effects [265].…”