Nanoparticle Tracking Analysis of Urinary Extracellular Vesicle Proteins as a New Challenge in Laboratory Medicine

Sałaga-Zaleska, Kornelia; Kuchta, Agnieszka; Bzoma, Beata; Chyła-Danił, Gabriela; Safianowska, Anna; Płoska, Agata; Kalinowski, Leszek; Dębska-Ślizień, Alicja; Jankowski, Maciej

doi:10.3390/ijms241512228

Cited by 4 publications

(2 citation statements)

References 46 publications

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“…Due to the above-mentioned advantages, the analysis and quantification of exosomes has become one of the widely explored topics for the purpose of early detection of diseases. Several methods have been developed for this purpose, such as nanoparticle tracking analysis [ 5 ], flow cytometry [ 6 ], dynamic light scattering [ 7 ], and Western blot [ 8 ]. However, because the process is time consuming and the operation requires skilled operators, mass sample testing is difficult to realize.…”

Section: Introductionmentioning

confidence: 99%

Advances in Nanoplasmonic Biosensors: Optimizing Performance for Exosome Detection Applications

Nurrohman,

Chiu,

Hsiao

et al. 2024

Biosensors

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The development of sensitive and specific exosome detection tools is essential because they are believed to provide specific information that is important for early detection, screening, diagnosis, and monitoring of cancer. Among the many detection tools, surface-plasmon resonance (SPR) biosensors are analytical devices that offer advantages in sensitivity and detection speed, thereby making the sample-analysis process faster and more accurate. In addition, the penetration depth of the SPR biosensor, which is <300 nm, is comparable to the size of the exosome, making the SPR biosensor ideal for use in exosome research. On the other hand, another type of nanoplasmonic sensor, namely a localized surface-plasmon resonance (LSPR) biosensor, has a shorter penetration depth of around 6 nm. Structural optimization through the addition of supporting layers and gap control between particles is needed to strengthen the surface-plasmon field. This paper summarizes the progress of the development of SPR and LSPR biosensors for detecting exosomes. Techniques in signal amplification from two sensors will be discussed. There are three main parts to this paper. The first two parts will focus on reviewing the working principles of each sensor and introducing several methods that can be used to isolate exosomes. This article will close by explaining the various sensor systems that have been developed and the optimizations carried out to obtain sensors with better performance. To illustrate the performance improvements in each sensor system discussed, the parameters highlighted include the detection limit, dynamic range, and sensitivity.

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Section: Introductionmentioning

confidence: 99%

Advances in Nanoplasmonic Biosensors: Optimizing Performance for Exosome Detection Applications

Nurrohman,

Chiu,

Hsiao

et al. 2024

Biosensors

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show abstract

“…Due to the above-mentioned advantages, analysis and quantification of exosomes has become one of the widely explored topics for the purpose of early detection of diseases. Several methods have been developed for this purpose such as nanoparticle tracking analysis [4], flow cytometry [5], dynamic light scattering [6], and western blot [7]. However, because the process is time consuming and the operation requires skilled operators, mass sample testing is difficult to realize.…”

Section: Introductionmentioning

confidence: 99%

Advances in Nanoplasmonic Biosensors: Optimizing Performance for Exosome Detection Applications

Nurrohman,

Chiu,

Hsiao

et al. 2024

Preprint

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The development of sensitive and specific exosome detection devices is highly desirable because it is believed to deliver specific important information for cancer screening, diagnosis, and monitoring. Among many detection techniques, nanoplasmonic sensors are optical detection methods that offer advantages in sensitivity and detection speed and this makes the sample analysis process faster and more accurate. This paper summarizes the development of nanoplasmonic biosensors, especially Surface Plasmon Resonance (SPR) and Localized Surface Plasmon Resonance (LSPR)-based biosensors for exosome detection. There are three main parts in this paper. The first part will focus on introducing the theoretical background regarding the working principles of SPR and LSPR biosensors. After that, we will introduce several methods that can be used for exosome isolation. This article will close by explaining the engineering of nanoplasmonic sensors both in terms of transducer modification to amplify the signal and functionalization strategies to increase sensor specificity. In this section, the detection mechanism, the type of exosomes detected, and the resulting detection limits are some of the points highlighted in this paper.

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Extracellular Vesicle Preparation and Analysis: A State‐of‐the‐Art Review

Wang,

Zhou,

Kong

et al. 2024

Advanced Science

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In recent decades, research on Extracellular Vesicles (EVs) has gained prominence in the life sciences due to their critical roles in both health and disease states, offering promising applications in disease diagnosis, drug delivery, and therapy. However, their inherent heterogeneity and complex origins pose significant challenges to their preparation, analysis, and subsequent clinical application. This review is structured to provide an overview of the biogenesis, composition, and various sources of EVs, thereby laying the groundwork for a detailed discussion of contemporary techniques for their preparation and analysis. Particular focus is given to state‐of‐the‐art technologies that employ both microfluidic and non‐microfluidic platforms for EV processing. Furthermore, this discourse extends into innovative approaches that incorporate artificial intelligence and cutting‐edge electrochemical sensors, with a particular emphasis on single EV analysis. This review proposes current challenges and outlines prospective avenues for future research. The objective is to motivate researchers to innovate and expand methods for the preparation and analysis of EVs, fully unlocking their biomedical potential.

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Nanoparticle Tracking Analysis of Urinary Extracellular Vesicle Proteins as a New Challenge in Laboratory Medicine

Cited by 4 publications

References 46 publications

Advances in Nanoplasmonic Biosensors: Optimizing Performance for Exosome Detection Applications

Advances in Nanoplasmonic Biosensors: Optimizing Performance for Exosome Detection Applications

Advances in Nanoplasmonic Biosensors: Optimizing Performance for Exosome Detection Applications

Extracellular Vesicle Preparation and Analysis: A State‐of‐the‐Art Review

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