Nanoparticle sizing in the field of nanomedicine: Power of an analytical ultracentrifuge

Cinar, Gizem; Solomun, Jana I.; Mapfumo, Prosper; Traeger, Anja; Nischang, Ivo

doi:10.1016/j.aca.2022.339741

Cited by 13 publications

(25 citation statements)

References 58 publications

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“…Figure A,B also show a comparison of the intensity-based distributions of hydrodynamic diameters from DLS, d h, DLS , with those based on sedimentation analysis, ls – g *( d h ), and sedimentation–diffusion analysis, c ( d h ), by including the translational frictional ratios, f / f sph (eq and Table S2). The differential distributions of hydrodynamic diameters from AUC, ls – g *( d h ) or c ( d h ), and resulting d h,AUC values were derived as reported recently . The z -average d h, DLS and average d h,AUC values from sedimentation–diffusion analysis practically coincide (Table S2).…”

Section: Resultsmentioning

confidence: 99%

“…The d h, DLS is obtained by cumulant analysis of the decay functions and allows estimation of diffusion coefficients, D , and consequently hydrodynamic diameters, d h, DLS (eq ). The latter are accompanied with a polydispersity index (PDI), setting into relation the standard deviation of the investigated population and the hydrodynamic diameters …”

Section: Methodsmentioning

confidence: 99%

“…Perhaps the most common way is the determination of d h values by dynamic light scattering (DLS) experiments. DLS originally assesses translational diffusion coefficients, D . ,, …”

Section: Theoretical Backgroundmentioning

confidence: 99%

“…Intrinsic estimates are particularly useful, because they allow for solution system comparability, i.e., when experiments in differently dense and viscous solvents are performed. ,, …”

Section: Theoretical Backgroundmentioning

confidence: 99%

“…A key technological platform for the absolute study of polymer systems is an analytical ultracentrifuge that found its inception with Svedberg’s pioneering work on disperse systems. , In combination with viscometry of macromolecular systems, first proposed by Staudinger, − the methods of macromolecular hydrodynamics have developed into powerful approaches in the study of polymers of natural and synthetic origin . The peculiar advantage of AUC emanates from the study of materials in solution under action of a centrifugal force in the hermetically closed system of conserved mass balance. − The materials are investigated by suitable detectors in solution without global dilution and absence of sample processing issues.…”

Section: Introductionmentioning

confidence: 99%

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PEG-Lipids: Quantitative Study of Unimers and Aggregates Thereof by the Methods of Molecular Hydrodynamics

2023

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Understanding the polymorphism of lipids in solution is the key to the development of intracellular delivery systems. Here, we study the dynamics of poly(ethylene glycol)-lipid (PEG-Lipid) conjugates aiming at a better understanding of their molecular properties and aggregation behavior in solution. Those PEG-Lipids are used as components of lipid nanoparticles (LNPs). LNPs are gaining increased popularity, e.g., by their utilization in modern vaccination strategies against SARS-CoV-2. Characterization of the systems is conducted by the classical methods of hydrodynamics in different solvents, such as ethanol and water, which are also commonly used for LNP formulation. We were able to elucidate the structurally associated hydrodynamic properties of isolated PEG-Lipids in ethanol, revealing the typically expected values of the hydrodynamic invariant for random coil polymers. By virtue of the same experimental setting, the PEG-Lipids' behavior in water was as well studied, which is a less good solvent than ethanol for the PEG-Lipids. Our experiments demonstrate that PEG-Lipids dissolved in water form welldefined micelles that can quantitatively be characterized in terms of their degree of aggregation of PEG-Lipid polymer unimers, their hydrodynamic size, and solvation, i.e., the quantitative determination of water contained or associated to the identified micelles. Quantitative results obtained from classical hydrodynamic analyses are fully supported by studies with standard dynamic light scattering (DLS). The obtained diffusion coefficients and hydrodynamic sizes are in excellent agreement with numerical results derived from analytical ultracentrifugation (AUC) data. Cryo-transmission electron microscopy (cryo-TEM) supports the structural insight from hydrodynamic studies, particularly, in terms of the observed spherical structure of the formed micelles. We demonstrate experimentally that the micelle systems can be considered as solvent-permeable, hydrated spheres.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Perhaps the most common way is the determination of d h values by dynamic light scattering (DLS) experiments. DLS originally assesses translational diffusion coefficients, D . ,, …”

Section: Theoretical Backgroundmentioning

confidence: 99%

“…Intrinsic estimates are particularly useful, because they allow for solution system comparability, i.e., when experiments in differently dense and viscous solvents are performed. ,, …”

Section: Theoretical Backgroundmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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PEG-Lipids: Quantitative Study of Unimers and Aggregates Thereof by the Methods of Molecular Hydrodynamics

2023

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Analysis of Macromolecular Systems as Enabler for Energy and Life Science Applications

Anufriev,

Debbeler,

Traeger

et al. 2024

Macro Chemistry & Physics

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Ideas concerning the conceptual existence of macromolecular and colloidal systems found their inception at the beginning of the last century. The experimental technology developed to discover and characterize those systems can be associated with seminal pioneers laying the foundations for microscopic, hydrodynamic, and light scattering approaches. In this perspective, we focus our attention on the origins of the discovery and characterization of macromolecular and colloidal systems with selected examples from the beginnings to the present. This perspective attempts to directly interconnect the design of new macromolecular as well as colloidal systems and the simultaneous development of using advanced characterization techniques for design verification. While not claiming a complete coverage of the entire field of modern polymer science, our selected examples concern the field of life science and the recently and rapidly developing area of energy materials.

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Rapid high-resolution size distribution protocol for adeno-associated virus using high speed SV-AUC

Berkowitz,

Larson,

Bou-Assaf

et al. 2024

Analytical Biochemistry

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Nanoparticle sizing in the field of nanomedicine: Power of an analytical ultracentrifuge

Cited by 13 publications

References 58 publications

PEG-Lipids: Quantitative Study of Unimers and Aggregates Thereof by the Methods of Molecular Hydrodynamics

PEG-Lipids: Quantitative Study of Unimers and Aggregates Thereof by the Methods of Molecular Hydrodynamics

Analysis of Macromolecular Systems as Enabler for Energy and Life Science Applications

Rapid high-resolution size distribution protocol for adeno-associated virus using high speed SV-AUC

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