Nanoparticle‐Mediated Delivery of Micheliolide Analogs to Eliminate Leukemic Stem Cells in the Bone Marrow (Adv. Therap. 1/2022)

Bone‐Targeted Nanoparticle Drug Delivery System‐Mediated Macrophage Modulation for Enhanced Fracture Healing

“…To form TBP-NP, ddH 2 O was added at a rate of 24 μL min −1 via syringe pump to TBP-PSMA-b-PS dissolved in 30 mL DMF (200 mg/30 mL), which was then dialyzed against water for 3 days (MWCO 6-8 kDa) and filtered using 0.2 μm sterile cellulose acetate filters. [10][11][12]16,17,19] Dynamic light scattering (DLS, Malvern Instrument, Worcestershire, UK) was used to determine the size and surface charge of TBP-NP. A Malvern Nanosight analysis further determined NP concentration to enable calculation of TBP-NP.…”

“…To this end, we previously established a bone-targeted NP DDS, where active bone targeting is achieved by incorporation of tartrate-resistant acid phosphatase (TRAP) binding peptide (TBP) to poly(styrene-alt-maleic anhydride)-b-poly(styrene) (PSMA-b-PS) to form TBP-NP. [10][11][12][13][14][15][16][17][18] We showed ≈3-fold greater fracture accumulation of TBP-NP versus untargeted NP, and by entrapping AR28, a glycogen synthase kinase-3 beta (GSK3𝛽) inhibitor, fracture healing was expedited versus untreated controls. Initially, the overarching conclusion of this work was that AR28 release at the fracture site upregulated Wnt-𝛽-catenin signaling and enhanced osteogenesis of osteoprogenitor cell types, such as mesenchymal stem cells.…”