Nanoparticle-based sorting of circulating tumor cells by epithelial antigen expression during disease progression in an animal model

Muhanna, Nidal; Mepham, Adam; Mohamadi, Reza M.; Chan, Harley; Khan, Tahsin M.; Akens, Margarete K.; Besant, Justin D.; Irish, Jonathan C.

doi:10.1016/j.nano.2015.04.017

Cited by 24 publications

(32 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This device can also be used to monitor CTCs in animal models of cancer, thus presenting am eans to better understand the dynamics of EMT ( Figure 5D). [84,86] As an alternative to events caused by single cells,C TCs could lead to metastases by breaking off from the tumor in clumps and traveling in clusters.C ell clusters are thought to initiate metastatic spread after they get stuck in capillaries. [87] Te chnologies such as the Cluster Chip [88] and gelatin nanocoating substrates [69] capture CTC clusters in 30 %o fb reast cancer patients.C TC clusters are heterogeneous and include both actively proliferating cells and quiescent cells.F urther work will be required to uncover the clinical significance of these approaches and the role of CTC heterogeneity in cancer progression.…”

Section: Identification Of Ctc Subpopulations and Visualizing Heterogmentioning

confidence: 99%

See 1 more Smart Citation

Beyond the Capture of Circulating Tumor Cells: Next‐Generation Devices and Materials

et al. 2015

Self Cite

View full text Add to dashboard Cite

Over the last decade, significant progress has been made towards the development of approaches that enable the capture of rare circulating tumor cells (CTCs) from the blood of cancer patients, a critical capability for noninvasive tumor profiling. These advances have leveraged new insights in materials chemistry and microfluidics and allowed the capture and enumeration of CTCs with unprecedented sensitivity. However, it has become increasingly clear that simply capturing and counting tumor cells launched into the bloodstream may not provide the information needed to advance our understanding of the biology of these rare cells, or to allow us to better exploit them in medicine. A variety of advances have now emerged demonstrating that more information can be extracted from CTCs with next-generation devices and materials featuring tailored physical and chemical properties. In this Minireview, the last ten years of work in this area will be discussed, with an emphasis on the groundbreaking work of the last five years, during which the focus has moved beyond the simple capture of CTCs and gravitated towards approaches that enable in-depth analysis.

show abstract

Section: Identification Of Ctc Subpopulations and Visualizing Heterogmentioning

confidence: 99%

“…CTC subpopulations have been visualized in human patients and tumor-bearinganimals by using this approach. [84] Figure adapted from Refs. [83,84,86].…”

Section: Integrated Molecular Analysis Of Ctcsmentioning

confidence: 99%

Beyond the Capture of Circulating Tumor Cells: Next‐Generation Devices and Materials

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…[83][84][85] CTCs,d ie mit magnetischen und mit anti-EpCAM-Antikçrpern beschichteten Nanopartikeln markiert sind, kçnnen entsprechend der Menge an einem Oberflächenantigen sortiert werden, von dem bekannt ist, dass es während der EMT abnimmt. Das Einfangen der Zellen mit Nanopartikeln funktioniert wegen der großen Zahl von Bindungsereignissen, die an der Oberfläche mçglich sind, gut;imkonkreten Fall stellt allerdings die geringe magnetische Suszeptibilität der Partikel eine Hürde dar.D aher wurden in den Chips X-fçrmige Mikrostrukturen erzeugt, durch die Regionen niedrigen Flusses entstehen (Abbildung 5B).…”

Section: Methodsunclassified

“…Mit diesem Chip kann man auch CTCs in Tiermodellen von Krebs verfolgen und so eine Mçglichkeit erçffnen, um die Dynamik der EMT besser zu verstehen (Abbildung 5D). [84,86] Als Alternative zu Ereignissen, die durch einzelne Zellen hervorgerufen werden, kçnnen CTCs auch in Form von Zellhaufen, die aus dem Tumor wegbrechen und als Cluster wandern, zu Metastasen führen. Bei Zellclustern vermutet man, dass sie Metastasen initiieren, wenn sie in Kapillaren steckenbleiben.…”

Section: Methodsunclassified

See 1 more Smart Citation

Profilierung zirkulierender Tumorzellen mit Apparaturen und Materialien der nächsten Generation

Green

Safaei²,

Mepham

et al. 2015

Angewandte Chemie

Self Cite

View full text Add to dashboard Cite

In den letzten zehn Jahren gab es wichtige Fortschritte bei der Erfassung seltener zirkulierender Tumorzellen (CTCs) aus dem Blut von Krebspatienten als eine entscheidende Voraussetzung für die nichtinvasive Tumorprofilierung. Diese Fortschritte haben zudem neue Einblicke in die Materialchemie und die Mikrofluidik erbracht und ermöglichten die Erfassung und Auszählung von CTCs mit beispielloser Empfindlichkeit. Allerdings wurde auch immer klarer, dass einfaches Isolieren und Auszählen von Tumorzellen, die in den Kreislauf gelangt waren, nicht die benötigten Informationen lieferte, um unser Verständnis der Biologie dieser seltenen Zellen zu vertiefen oder um sie sich in der Therapie besser zunutze zu machen. Mithilfe von Apparaturen und Materialien der nächsten Generation mit maßgeschneiderten physikalischen und chemischen Eigenschaften ist man inzwischen in der Lage, mehr Informationen aus CTCs zu gewinnen. In diesem Kurzaufsatz werden die Arbeiten der letzten zehn Jahre in diesem Gebiet diskutiert, wobei insbesondere die bahnbrechenden Studien der letzten fünf Jahre Berücksichtigung finden, die den Blick über das einfache Isolieren von CTCs hinaus auf Ansätze gerichtet haben, die eine tiefergehende Analyse ermöglichen.

show abstract

Recent advances in microfluidic cell sorting techniques based on both physical and biochemical principles

Tang

Jiang

et al. 2018

Electrophoresis

View full text Add to dashboard Cite

Microfluidic technologies for isolating cells of interest from a heterogeneous sample have attracted great attentions, due to the advantages of less sample consumption, simple operating procedure, and high separation accuracy. According to the working principles, the microfluidic cell sorting techniques can be categorized into biochemical (labeled) and physical (label‐free) methods. However, the inherent drawbacks of each type of method may somehow influence the popularization of these cell sorting techniques. Using the multiple complementary isolation principles is a promising strategy to overcome this problem, therefore there appears to be a continuing trend to integrate two or more sorting methods together. In this review, we focus on the recent advances in microfluidic cell sorting techniques relied on both physical and biochemical principles, with emphasis on the mechanisms of cell separation. The biochemical cell sorting techniques enhanced by physical principles and the physical cell sorting techniques enhanced by biochemical principles, are first introduced. Then, we highlight on‐chip magnetic‐activated cell sorting, on‐chip fluorescence‐activated cell sorting, multi‐step cell sorting and multi‐principle cell sorting techniques, which are based on both physical and biochemical separation mechanisms. Finally, the challenges and future perspectives of the integrated microfluidics for cell sorting are discussed.

show abstract

Nanoparticle-based sorting of circulating tumor cells by epithelial antigen expression during disease progression in an animal model

Cited by 24 publications

References 27 publications

Beyond the Capture of Circulating Tumor Cells: Next‐Generation Devices and Materials

Beyond the Capture of Circulating Tumor Cells: Next‐Generation Devices and Materials

Profilierung zirkulierender Tumorzellen mit Apparaturen und Materialien der nächsten Generation

Recent advances in microfluidic cell sorting techniques based on both physical and biochemical principles

Contact Info

Product

Resources

About