Nanomaterial-mediated photoporation for intracellular delivery

Wang, Li; Wei, Xinbo; Liu, Haifeng; Fan, Yubo

doi:10.1016/j.actbio.2022.12.050

Cited by 10 publications

(6 citation statements)

References 251 publications

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“…VBs diffuse and collapse rapidly after absorption of laser light, followed by intensive shock waves and fluid shear stress to form pores on the cell membrane. [46,50,51,53,55,56] Additionally, nanoplasma and nanobubbles can be generated upon amplification of the electromagnetic field around AuNPs induced by an off-resonance near The laser pulse heats up the nanobomb to evaporate surrounding water and cause the formation of VBs. Then the VBs collapse and exert mechanical forces to the cell membrane and create pores, which enable foreign molecules to pass through.…”

Section: Optoporationmentioning

confidence: 99%

Section: Optoporationmentioning

confidence: 99%

“…VBs diffuse and collapse rapidly after absorption of laser light, followed by intensive shock waves and fluid shear stress to form pores on the cell membrane. [ 46,50,51,53,55,56 ] Additionally, nanoplasma and nanobubbles can be generated upon amplification of the electromagnetic field around AuNPs induced by an off‐resonance near infrared (NIR) femtosecond laser; pores are formed while leaving AuNPs intact. [ 42 ] Schomaker's team concluded this as “nanolens effect” in that at this wavelength both the absorption of AuNPs and the thermal impact on AuNPs are reduced, while at resonant wavelength the morphology can be changed.…”

Section: The Patterns and Mechanisms Of Cell Membrane Perforationmentioning

confidence: 99%

mentioning

confidence: 99%

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Cell Membrane Perforation: Patterns, Mechanisms and Functions

Zhu,

Shi,

Mao

et al. 2024

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Cell membrane is crucial for the cellular activities, and any disruption to it may affect the cells. It is demonstrated that cell membrane perforation is associated with some biological processes like programmed cell death (PCD) and infection of pathogens. Specific developments make it a promising technique to perforate the cell membrane controllably and precisely. The pores on the cell membrane provide direct pathways for the entry and exit of substances, and can also cause cell death, which means reasonable utilization of cell membrane perforation is able to assist intracellular delivery, eliminate diseased or cancerous cells, and bring about other benefits. This review classifies the patterns of cell membrane perforation based on the mechanisms into 1) physical patterns, 2) biological patterns, and 3) chemical patterns, introduces the characterization methods and then summarizes the functions according to the characteristics of reversible and irreversible pores, with the aim of providing a comprehensive summary of the knowledge related to cell membrane perforation and enlightening broad applications in biomedical science.

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Section: Optoporationmentioning

confidence: 99%

Section: Optoporationmentioning

confidence: 99%

“…VBs diffuse and collapse rapidly after absorption of laser light, followed by intensive shock waves and fluid shear stress to form pores on the cell membrane. [ 46,50,51,53,55,56 ] Additionally, nanoplasma and nanobubbles can be generated upon amplification of the electromagnetic field around AuNPs induced by an off‐resonance near infrared (NIR) femtosecond laser; pores are formed while leaving AuNPs intact. [ 42 ] Schomaker's team concluded this as “nanolens effect” in that at this wavelength both the absorption of AuNPs and the thermal impact on AuNPs are reduced, while at resonant wavelength the morphology can be changed.…”

Section: The Patterns and Mechanisms Of Cell Membrane Perforationmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Cell Membrane Perforation: Patterns, Mechanisms and Functions

Zhu,

Shi,

Mao

et al. 2024

Small

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“…These include electroporation [ 10 , 11 ], sonoporation [ 12 ], and photoporation [ 13 – 15 ]. Many studies have reported an increase in the efficiency of membrane disruption using plasmonic nanoparticles [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Upconverting-photon quenching-mediated perforation influx as an intracellular delivery method using posAuNP@UCNPs nanocomposites for osteoarthritis treatment

Kim,

Cho,

Kim

et al. 2024

Nano Convergence

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Photoporation techniques based on plasmonic nanoparticles such as gold nanoparticles have been extensively studied for the intracellular delivery of substances via cell membrane disruption. However, the clinical application of AuNP is challenging due to its absorption in the 500 nm region of the light spectrum. To overcome this challenge, upconversion nanoparticles were employed to stimulate AuNP at NIR wavelengths. posAuNP@UCNPs nanocomposites were produced by coating 30 nm UCNPs on 80 nm AuNPs using DOPA-PEI, which were then irradiated with 980 nm NIR light to facilitate their intracellular delivery. TEM and DLS confirmed that posAuNP and UCNP combine to form nanocomposites. Additionally, multiphysics simulation was used to analyze the distribution of the posAuNP electric field based on morphological differences that change as the UCNP ratio increases. Next, effective LED irradiation conditions were established by applying upconverting-photon quenching-mediated perforation influx to C28/I2 cells as suspensions or spheroids. posAuNP@UCNP nanocomposites were confirmed to be effective for the delivery of baricitinib as a treatment for osteoarthritis in a three-dimensional osteoarthritis model. Finally, chondrocyte differentiation was induced through intracellular delivery of baricitinib using posAuNP@UCNPs. The findings suggest that posAuNP@UCNPs have great potential as a tool for non-invasive drug delivery via UCPPin. Graphical Abstract

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Enhanced Cellular Uptake through Nanotopography‐Induced Macropinocytosis

Aramesh,

Yu,

Essand

et al. 2024

Adv Funct Materials

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Efficient cellular uptake of biomolecules, including genetic material, mRNA, proteins, and nanoparticles, requires novel approaches to overcome inherent cellular barriers. Here, the study investigates how nanotopographical cues from nanoporous surfaces impact the uptake efficiency by cells. The results demonstrate notable enhancements in cellular uptake efficiency across a range of vectors when cells are exposed to nanoporous surfaces. The uptake process is found to be dependent on the size and morphology of the nanopores, reaching a peak efficacy with blind pores of 400 nm in size. Enhanced genetic transduction on nanoporous surfaces are observed for multiple vectors, including lentiviruses, baculoviruses, and mRNA molecules. The versatile nature of this approach allows co‐transfection of cells with multiple mRNA vectors. Moreover, the nanoporous platform is used for efficient and fast manufacturing of Chimeric Antigen Receptor (CAR)‐T cells through lentiviral transduction. Furthermore, the study pinpoints macropinocytosis as the predominant mechanism driving increased cellular uptake induced by the nanoporous surfaces. The introduced method for enhancing genetic transduction of cells has applications in immunotherapy research, drug delivery, and cell engineering.

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Nanomaterial-mediated photoporation for intracellular delivery

Cited by 10 publications

References 251 publications

Cell Membrane Perforation: Patterns, Mechanisms and Functions

Cell Membrane Perforation: Patterns, Mechanisms and Functions

Upconverting-photon quenching-mediated perforation influx as an intracellular delivery method using posAuNP@UCNPs nanocomposites for osteoarthritis treatment

Enhanced Cellular Uptake through Nanotopography‐Induced Macropinocytosis

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