Nanohydroxyapatite increases BMP-2 expression via a p38 MAP kinase dependent pathway in periodontal ligament cells

Suto, Mizuki; Nemoto, Eiji; Kanaya, Sousuke; Suzuki, Ryuichi; Tsuchiya, Masahiro; Shimauchi, Hidetoshi

doi:10.1016/j.archoralbio.2013.02.014

Cited by 28 publications

(23 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, combining the different scaffold fabrication methods with nanotechnology can provide new and improved biomaterials. It has also been reported that nano-HAp selectively increased the expression of BMP-2 in dose and time-dependent manners at mRNA and protein levels, in human periodontal ligament cells [20]. Furthermore, concentrations of Ca 2+ and PO 4 2- were not changed in cell culture supernatants, suggesting that nano-HAp functioned as a nanoparticle rather than as a possible extracellular source of Ca 2+ and/or PO 4 2- [20].…”

Section: Introductionmentioning

confidence: 98%

“…It has also been reported that nano-HAp selectively increased the expression of BMP-2 in dose and time-dependent manners at mRNA and protein levels, in human periodontal ligament cells [20]. Furthermore, concentrations of Ca 2+ and PO 4 2- were not changed in cell culture supernatants, suggesting that nano-HAp functioned as a nanoparticle rather than as a possible extracellular source of Ca 2+ and/or PO 4 2- [20]. This novel mechanism explaining the action of nano-HAp encouraged the development of new strategies for periodontal intrabony defects treatment using nanoparticles [5,18,20].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bioactive Polymeric Nanoparticles for Periodontal Therapy

et al. 2016

View full text Add to dashboard Cite

Aimsto design calcium and zinc-loaded bioactive and cytocompatible nanoparticles for the treatment of periodontal disease.MethodsPolymP-nActive nanoparticles were zinc or calcium loaded. Biomimetic calcium phosphate precipitation on polymeric particles was assessed after 7 days immersion in simulated body fluid, by scanning electron microscopy attached to an energy dispersive analysis system. Amorphous mineral deposition was probed by X-ray diffraction. Cell viability analysis was performed using oral mucosa fibroblasts by: 1) quantifying the liberated deoxyribonucleic acid from dead cells, 2) detecting the amount of lactate dehydrogenase enzyme released by cells with damaged membranes, and 3) by examining the cytoplasmic esterase function and cell membranes integrity with a fluorescence-based method using the Live/Dead commercial kit. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests.ResultsPrecipitation of calcium and phosphate on the nanoparticles surfaces was observed in calcium-loaded nanoparticles. Non-loaded nanoparticles were found to be non-toxic in all the assays, calcium and zinc-loaded particles presented a dose dependent but very low cytotoxic effect.ConclusionsThe ability of calcium-loaded nanoparticles to promote precipitation of calcium phosphate deposits, together with their observed non-toxicity may offer new strategies for periodontal disease treatment.

show abstract

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Bioactive Polymeric Nanoparticles for Periodontal Therapy

et al. 2016

View full text Add to dashboard Cite

show abstract

“…This synergistic effect can significantly improve the bone healing process in a variety of clinical cases. In particular, the outcomes of the in vitro tests, along with the well‐known HA effect on several genes involved in osteogenesis, and along with the proved significant increase in bone calcium content occurring in gallium‐treated bone (reducing the osteoclast activity), demonstrate the promising features of these multi‐substituted apatites in bone homeostasis regulation in degenerative diseases like osteoporosis.…”

Section: Discussionmentioning

confidence: 93%

New hydroxyapatite nanophases with enhanced osteogenic and anti‐bacterial activity

Ballardini

Montesi

Panseri

et al. 2017

J Biomedical Materials Res

View full text Add to dashboard Cite

This work describes the synthesis and characterization of new apatite phases co-doped with gallium, magnesium and carbonate, exhibiting osteogenic and antibacterial ability. The apatites are synthesized at low temperature to retain nanocrystallinity and controlled doping with the various bioactive foreign ions, as assessed by physico-chemical and crystallographic analyses, reporting the achievement of single phases with reduced crystal ordering. The analysis of single and multi-doped apatites reports to different mechanisms acting in the incorporation of gallium and magnesium ions in the apatite structure. The release of bioactive ions is correlated to the behavior of human mesenchymal stem cells and of different bacterial strands, selected among the most frequently affecting surgical procedures. Enhanced osteogenic and antibacterial ability is assessed in multi-doped apatites, thus suggesting potential future applications as new smart biomaterials integrating a significant boosting of bone regeneration with adequate protection against bacteria. © 2017 Wiley Periodicals Inc. J Biomed Mater Res Part A: 106A: 521-530, 2018.

show abstract

“…Subsequently, membranous osteogenesis increased in and around the HAp granules, leading to the formation of bone-like structures, with periosteal and tendon-like structures, bone marrow, and focal chondrogenesis. To explain the mechanism by which nHAp stimulates osteoinduction, Suto et al [20] demonstrated that nHAp increases BMP-2 expression at both the gene and protein level through activation of the p38 mitogen-activated protein kinase pathway. In light of these discoveries, our results of immunohistochemistry, which showed the expression of osteocalcin, osteonectin and osteopontin, support the osteoinduction property of nHAp.…”

Section: Discussionmentioning

confidence: 99%

Biologic properties of nano-hydroxyapatite: An in vivo study of calvarial defects, ectopic bone formation and bone implantation

Pang

Lee

Seo

et al. 2015

Bio-Medical Materials and Engineering

View full text Add to dashboard Cite

This study investigated the biologic properties of nano-hydroxyapatite (nHAp) using the rat calvarial defect, ectopic bone formation, and rabbit tibia implant installation models. Animals were divided into two groups: those implanted with nHAp, and negative controls (Collagen). Eight weeks after creating an 8 mm calvarial defect, bone regeneration was evaluated radiographically and histologically. To investigate ectopic bone formation, materials were injected into the right thigh muscle and were evaluated after 8 weeks. nHAp coated implant and conventional titanium implant were placed bilaterally in rabbit tibias. After 4 weeks, bone-implant contact (BIC), new bone area inside the thread, and removal torque were measured. In the calvarial defect model, radiographic and histologic analysis showed more bone formation in the nHAp Group; particularly, histologically assessed bone area (p = 0.034) and microcomputed tomography assessed bone mineral density (p = 0.034). In the ectopic bone formation model, calcification and expression of osteogenic biomarkers were seen in the nHAp-injected samples but in none of the controls. nHAp coated implant resulted in increased BIC, new bone area, and increased removal torque, with statistical significance for BIC (p = 0.034). This study suggests that nHAp has potential as a coating material for dental implant surfaces and as a bone graft material.

show abstract

Nanohydroxyapatite increases BMP-2 expression via a p38 MAP kinase dependent pathway in periodontal ligament cells

Cited by 28 publications

References 30 publications

Bioactive Polymeric Nanoparticles for Periodontal Therapy

Bioactive Polymeric Nanoparticles for Periodontal Therapy

New hydroxyapatite nanophases with enhanced osteogenic and anti‐bacterial activity

Biologic properties of nano-hydroxyapatite: An in vivo study of calvarial defects, ectopic bone formation and bone implantation

Contact Info

Product

Resources

About