Nanogels designed for cell-free nucleic acid sequestration

Huang, Yuhang; Li, Shangyu; Zettle, Logan W. C.; Ma, Yingshan; Naguib, Hani E.; Kumacheva, Eugenia

doi:10.1039/d3nr03231k

Cited by 7 publications

(9 citation statements)

References 62 publications

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“…30 These data suggest that the use of surface positively charged nanoparticles as nanocarriers enabled electrostatically driven nanoparticle scavenging, thereby improving drug accumulation inside the cells and thus optimum therapeutic efficacy. 31 Effects of Surface Chemical Moieties on Nanoparticle Release. Exocytosis rates determine the residency time of nanoparticles in the cell, which, in turn, determines the toxicity profile of nanoparticles to cells.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…A study also reported that amino-functionalized polymeric nanoparticles could retain intracellularly for up to 8 days without altering the physiological functions of the cell . These data suggest that the use of surface positively charged nanoparticles as nanocarriers enabled electrostatically driven nanoparticle scavenging, thereby improving drug accumulation inside the cells and thus optimum therapeutic efficacy …”

Section: Resultsmentioning

confidence: 99%

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Effects of Different Surface Functionalizations of Silica Nanoparticles on Mesenchymal Stem Cells

Huang,

Zhou,

et al. 2024

ACS Appl. Bio Mater.

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Physicochemical properties of nanoparticles, such as particle size, surface charge, and particle shape, have a significant impact on cell activities. However, the effects of surface functionalization of nanoparticles with small chemical groups on stem cell behavior and function remain understudied. Herein, we incorporated different chemical functional groups (amino, DETA, hydroxyl, phosphate, and sulfonate with charges of +9.5, + 21.7, −14.1, −25.6, and −37.7, respectively) to the surface of inorganic silica nanoparticles. To trace their effects on mesenchymal stem cells (MSCs) of rat bone marrow, these functionalized silica nanoparticles were used to encapsulate Rhodamine B fluorophore dye. We found that surface functionalization with positively charged and short-chain chemical groups facilitates cell internalization and retention of nanoparticles in MSCs. The endocytic pathway differed among functionalized nanoparticles when tested with ion-channel inhibitors. Negatively charged nanoparticles mainly use lysosomal exocytosis to exit cells, while positively charged nanoparticles can undergo endosomal escape to avoid scavenging. The cytotoxic profiles of these functionalized silica nanoparticles are still within acceptable limits and tolerable. They exerted subtle effects on the actin cytoskeleton and migration ability. Last, phosphate-functionalized nanoparticles upregulate osteogenesis-related genes and induce osteoblast-like morphology, implying that it can direct MSCs lineage specification for bone tissue engineering. Our study provides insights into the rational design of biomaterials for effective drug delivery and regenerative medicine.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Effects of Different Surface Functionalizations of Silica Nanoparticles on Mesenchymal Stem Cells

Huang,

Zhou,

et al. 2024

ACS Appl. Bio Mater.

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“…Polysaccharide particles can be formed by physical or chemical crosslinking of PS molecules. Physical crosslinking can be achieved via hydrogen bonding, [94] ionic crosslinking, [95] hydrophobic interactions, [96] or host-guest interactions. [97] Figure 4, top row, shows images of exemplary PSPs and specifies [94] Copyright 2018, ACS.…”

Section: Particle Formation By Physical Crosslinking Of Polysaccharid...mentioning

confidence: 99%

Section: Particle Formation By Physical Crosslinking Of Polysaccharid...mentioning

confidence: 99%

“…[102] For instance, Figure 4b shows nanogels formed from quaternary ammonium cation-modified chitosan molecules that were crosslinked by sodium triphosphate. [95] Despite the efficiency and convenience of ionic crosslinking, network formation is reversible through changes in solvent ionic strength and pH. For example, crosslinked nanogels formed by anionic alginate and cationic doxorubicin disassembled when alginate molecules were protonated under acidic pH.…”

Section: Particle Formation By Physical Crosslinking Of Polysaccharid...mentioning

confidence: 99%

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From Nature‐Sourced Polysaccharide Particles to Advanced Functional Materials

Ma,

Morozova,

Kumacheva

2024

Advanced Materials

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Polysaccharides constitute over 90% of the carbohydrate mass in nature, which makes them a promising feedstock for manufacturing sustainable materials. Polysaccharide particles (PSPs) are used as effective scavengers, carriers of chemical and biological cargo, and as building blocks for the fabrication of macroscopic materials. The biocompatibility and degradability of PSPs are advantageous for their uses as biomaterials with more environmental friendliness. This review highlights the progresses in PSP applications as advanced functional materials, by describing PSP extraction, preparation, and surface functionalization with a variety of functional groups, polymers, nanoparticles, and biologically active species. This review also outlines the fabrication of PSP‐derived materials, as well as their applications in soft robotics, sensing, scavenging, water harvesting, drug delivery, and bioengineering. The paper is concluded with an outlook providing perspectives in the development and applications of PSP‐derived materials.This article is protected by copyright. All rights reserved

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Nanotechnology‐Mediated Immunomodulation Strategy for Inflammation Resolution

Li,

Liu,

Wang

et al. 2024

Adv Healthcare Materials

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Inflammation serves as a common characteristic across a wide range of diseases and plays a vital role in maintaining homeostasis. Inflammation can lead to tissue damage and the onset of inflammatory diseases. Although significant progress is made in anti‐inflammation in recent years, the current clinical approaches mainly rely on the systemic administration of corticosteroids and antibiotics, which only provide short‐term relief. Recently, immunomodulatory approaches have emerged as promising strategies for facilitating the resolution of inflammation. Especially, the advanced nanosystems with unique biocompatibility and multifunctionality have provided an ideal platform for immunomodulation. In this review, the pathophysiology of inflammation and current therapeutic strategies are summarized. It is mainly focused on the nanomedicines that modulate the inflammatory signaling pathways, inflammatory cells, oxidative stress, and inflammation targeting. Finally, the challenges and opportunities of nanomaterials in addressing inflammation are also discussed. The nanotechnology‐mediated immunomodulation will open a new treatment strategy for inflammation therapy.

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Nanogels designed for cell-free nucleic acid sequestration

Abstract: Chronic wounds exhibit over-expression of cell-free deoxyribonucleic acid (cfDNA), leading to a prolonged inflammation and non-healing wounds. Scavenging excessive cfDNA molecules is a promising strategy for chronic wound treatment. Nanoparticles...

Cited by 7 publications

References 62 publications

Effects of Different Surface Functionalizations of Silica Nanoparticles on Mesenchymal Stem Cells

Effects of Different Surface Functionalizations of Silica Nanoparticles on Mesenchymal Stem Cells

From Nature‐Sourced Polysaccharide Particles to Advanced Functional Materials

Nanotechnology‐Mediated Immunomodulation Strategy for Inflammation Resolution

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