Nanofluidics for chemical and biological dynamics in solution at the single molecular level

Chantipmanee, Nattapong; Xu, Yan

doi:10.1016/j.trac.2022.116877

Cited by 13 publications

(16 citation statements)

References 127 publications

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“…Reproduced with permission 11 Copyright 2022, American Chemical Society liquid properties. 20,31,64,[80][81][82][83][84][85] Therefore, nanofluidics with these new phenomena present great opportunities to explore new scientific insights and applications of fluids. Because nanofluidics has dimensions comparable to those of single nanoscale objects, they provide a potential to sample small single nanoscale objects, such as, biomacromolecules, small organisms, subcellular matter, microvesicles, bacteria, and viruses.…”

Section: Toward Nanofluidics-based Mass Spectrometrymentioning

confidence: 99%

“…Nanofluidics, an evolving field of fully developed microfluidics, has garnered a great deal of attention, which has contributed to its recent expansion 38,40,78,79 . New physical phenomena, which are not observed in microfluidics, are dominant such as nonlinear transportation and altered liquid properties 20,31,64,80‐85 . Therefore, nanofluidics with these new phenomena present great opportunities to explore new scientific insights and applications of fluids.…”

Section: Toward Nanofluidics‐based Mass Spectrometrymentioning

confidence: 99%

“…Nanofluidics is the study and application of fluids confined within nanostructures. [20][21][22][23][24]41 With the advancement of nanofab-rication over the past two decades, [25][26][27][28][29][30][31][32][33]34,35 nanostructures with various nanofluidic geometries such as nanopipettes, nanotubes, nanopores, and nanochannels have been fabricated, resulting in diverse abilities and great potential for a variety of applications. In particular, the use of these nanofluidic geometries allows the precise handling of fluid samples with ultrasmall volumes ranging from picoliter to zeptoliter (10 −21 L, zl) order.…”

Section: Introductionmentioning

confidence: 99%

“…We believe that nanofluidics can be a promising tool in addressing this critical issue. Nanofluidics is the study and application of fluids confined within nanostructures 20‐24,41 . With the advancement of nanofabrication over the past two decades, 25‐33,34,35 nanostructures with various nanofluidic geometries such as nanopipettes, nanotubes, nanopores, and nanochannels have been fabricated, resulting in diverse abilities and great potential for a variety of applications.…”

Section: Introductionmentioning

confidence: 99%

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Toward nanofluidics‐based mass spectrometry for exploring the unknown complex and heterogeneous subcellular worlds

Chantipmanee¹,

2022

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Exploring unknown matter in an ultrasmall volume object, such as unknown subcellular matter in a single cell, requires an analytical technique that identifies unknown matter in terms of molecular structures and properties. Mass spectrometry is considered one of the best techniques for such analyses because it can identify unknown matter according to its mass‐to‐charge ratio. However, the use of mass spectrometry to identify unknown substances in such a small world has been greatly impeded due to the lack of tools to sample complex and heterogeneous analytes with ultrasmall volumes of the picoliter order, which is the volume order of a mammalian cell. We believe that nanofluidics would be an ideal tool to resolve this critical issue owing to its ability to sample such fluid samples with ultrasmall volumes ranging from the picoliter to zeptoliter order. Thus, the integration of such nanofluidic features into mass spectrometers would open up future avenues for the potential of mass spectrometry to explore unknown subcellular matter at a nano scale. In this perspective, we first discuss the applicability of microfluidics/nanofluidics to mass spectrometry, then address critical issues toward nanofluidics‐based mass spectrometry, and finally depict a personal outlook on the future of this field to resolve challenges on global and universal scales.

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Section: Toward Nanofluidics-based Mass Spectrometrymentioning

confidence: 99%

Section: Toward Nanofluidics‐based Mass Spectrometrymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Toward nanofluidics‐based mass spectrometry for exploring the unknown complex and heterogeneous subcellular worlds

Chantipmanee¹,

2022

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“…To resolve this challenge, we focus on chip-based nanofluidic devices (hereafter referred to as “nanofluidic devices”), which are solid-state devices containing in-plane nanochannels with a precisely controlled geometry. ,− In comparison with popular microfluidic devices, nanofluidic devices are at a nascent stage but possess great potential owing to their special phenomena and nanoscale effects. − In particular, owing to the ultrasmall volumes of the nanochannels, nanofluidic devices can confine single molecules at high concentrations ranging from nM to μM, which is the concentration range of reactant molecules in most chemical syntheses and biomolecules in a single cell. ,, In addition, owing to their planar, transparent, in-plane, and solid-state characteristics, nanofluidic devices can easily be coupled with a variety of microscopes and exhibit flexibility superior to that of other nanofluidic geometries, such as silicon-based nanofunnels, carbon nanotubes, nanopores, nanopipettes, nanoporous polymer membranes, and two-dimensional material membranes . Hence, nanofluidic devices have been used to manipulate and simultaneously visualize single biomacromolecules, such as DNA and proteins .…”

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confidence: 99%

Flexible Glass-Based Hybrid Nanofluidic Device to Enable the Active Regulation of Single-Molecule Flows

Kawagishi¹,

Funano

Tanaka

et al. 2023

Nano Lett.

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Single-molecule studies offer deep insights into the essence of chemistry, biology, and materials science. Despite significant advances in single-molecule experiments, the precise regulation of the flow of single small molecules remains a formidable challenge. Herein, we present a flexible glass-based hybrid nanofluidic device that can precisely block, open, and direct the flow of single small molecules in nanochannels. Additionally, this approach allows for real-time tracking of regulated single small molecules in nanofluidic conditions. Therefore, the dynamic behaviors of single small molecules confined in different nanofluidic conditions with varied spatial restrictions are clarified. Our device and approach provide a nanofluidic platform and mechanism that enable single-molecule studies and applications in actively regulated fluidic conditions, thus opening avenues for understanding the original behavior of individual molecules in their natural forms and the development of single-molecule regulated chemical and biological processes in the future.

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Nanofluidic Aptamer Nanoarray to Enable Stochastic Capture of Single Proteins at Normal Concentrations

Yang

Kamai

Wang

et al. 2023

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Single‐molecule experiments allow understanding of the diversity, stochasticity, and heterogeneity of molecular behaviors and properties hidden by conventional ensemble‐averaged measurements. They hence have great importance and significant impacts in a wide range of fields. Despite significant advances in single‐molecule experiments at ultralow concentrations, the capture of single molecules in solution at normal concentrations within natural biomolecular processes remains a formidable challenge. Here, a high‐density, well‐defined nanofluidic aptamer nanoarray (NANa) formed via site‐specific self‐assembly of well‐designed aptamer molecules in nanochannels with nano‐in‐nano gold nanopatterns is presented. The nanofluidic aptamer nanoarray exhibits a high capability to specifically capture target proteins (e.g., platelet‐derived growth factor BB; PDGF‐BB) to form uniform protein nanoarrays under optimized nanofluidic conditions. Owing to these fundamental features, the nanofluidic aptamer nanoarray enables the stochastic capture of single PDGF‐BB molecules at a normal concentration from a sample with a ultrasmall volume equivalent to a single cell by following Poisson statistics, forming a readily addressable single‐protein nanoarray. This approach offers a methodology and device to surpass both the concentration and volume limits of single‐protein capture in most conventional methodologies of single‐molecule experiments, thus opening an avenue to explore the behavior of individual biomolecules in a manner close to their natural forms, which remains largely unexplored to date.

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Nanofluidics for chemical and biological dynamics in solution at the single molecular level

Cited by 13 publications

References 127 publications

Toward nanofluidics‐based mass spectrometry for exploring the unknown complex and heterogeneous subcellular worlds

Toward nanofluidics‐based mass spectrometry for exploring the unknown complex and heterogeneous subcellular worlds

Flexible Glass-Based Hybrid Nanofluidic Device to Enable the Active Regulation of Single-Molecule Flows

Nanofluidic Aptamer Nanoarray to Enable Stochastic Capture of Single Proteins at Normal Concentrations

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