Nano silver: a novel nanomaterial for removal of bacterial contaminants in valerian (Valeriana officinalis L.) tissue culture

Abstract

Bacterial contamination is a serious problem in plant tissue culture procedures. An experiment was conducted to evaluate the potential of nano silver (NS) to remove bacterial contaminants of valerian nodal explants. This experiment was conducted as a completely randomized design in a factorial arrangement with four replications and each replicate with ten explants. Treatments involved NS at two stages (before and after surface sterilization along with control) with three rates (25, 50 and 100 mg l -1 ) at three times of soaking (30, 60 and 180 min). Explants were cultured on MS medium supplemented with 5 mg l -1 Kin and 0.1 mg l -1 NAA. Results showed that using 100 mg l -1 of NS solution after surface sterilization resulted in the highest percentage (89%) of disinfected explants. Nano silver solution did not affect the characters measured. On the basis of the data obtained in this experiment, it was concluded that NS had a good potential for removing of the bacterial contaminants in plant tissue culture procedures. As this is the first report on application of NS in in vitro culture techniques, further investigations on other plant species are needed to clarify the effectiveness of NS for the removal of bacterial contaminants in tissue culture of other crops.

RAPD Fingerprint to Appraise the Genetic Fidelity of In Vitro Propagated Araucaria excelsa R. Br. var. glauca Plantlets

“…glauca were chosen for this study. Pieces of orthotropic stem were cut from the 8 cm upper half and prewashed in tap water for 3 h. Owing to high contamination rate observed nano silver [19,20] was used to eradicate the internal bacteria with a concentration of 500 lm/ml under reduced pressure (300 mm Hg in 5 min).Then, explants were treated with 70% ethanol for 3 min and 15% Clorox (containing 5.25% sodium hypochlorite) with 0.2% household detergent for at least 10-20 min for surface sterilization, and then rinsed six times with sterilized distilled water. The stem pieces were then cut into 7-9 mm long explant and placed with their proximal ends on MS [21] basal medium with 3.0% sucrose and 0.8% agar.…”

Production of putrescine-capped stable silver nanoparticle: its characterization and antibacterial activity against multidrug-resistant bacterial strains

“…Albeit, recently silver nanoparticles have been studied as biomarkers (Jain et al 2008;Schoefield et al 2006) for their localized surface plasmon resonance. They are mostly popular for their antimicrobial applications like, wound dressings (Tian et al 2007), water treatment (Lv et al 2009), fabrics and textile processing (Vo Ke Thanh and Phuong Phong 2009) and more recently in agronomy (Mandeh et al 2012;Abdi et al 2008;Saha et al 2015). Silver in their nano dimension has been reported to show immense antimicrobial activity without generation of any resistant variety strains.…”

Antifungal activity of silver nanoparticles during in-vitro culture of Stevia rebaudiana Bertoni

“…The microbicidal effect of AgNPs derives from the interaction of silver ions with a broad range of molecular and metabolic processes within organisms, including growth inhibition, cell death, and inhibition of DNA replication (Abdi et al 2008; Yun’an Qing et al 2018). In fungi, AgNPs break the cell membrane of hyphae, thus impairing infection mechanisms (Kim et al 2008; Bocate et al 2019) and inhibiting the germination of conidia (Kim et al 2009).…”