Nano-fibrous scaffolds for tissue engineering

Smith, Laura A.; Ma, Peter

doi:10.1016/j.colsurfb.2003.12.004

Cited by 591 publications

(360 citation statements)

References 38 publications

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“…86 Similar to microscale fabrication, a wide variety of nanofabrication techniques have emerged in recent years. Examples of successful 2D and 3D nanofabrication techniques include electrospinning, [195][196][197][198][199] nanotemplating, 179,200 electrostatic spray deposition, 201,202 RF plasma spray/deposition, 203 molecular self assembly, [204][205][206] and metal oxide anodization. 104,182,[207][208][209] Of the fabrication techniques listed above, electrospinning has emerged as a very promising strategy for fabricating bone-mimetic nanofiber scaffolds.…”

Section: Physical Effectors In Synthetic Bone Scaffoldsmentioning

confidence: 99%

Bone tissue engineering: A review in bone biomimetics and drug delivery strategies

Porter

Ruckh

Popat

2009

Biotechnology Progress

672

499

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Critical‐sized defects in bone, whether induced by primary tumor resection, trauma, or selective surgery have in many cases presented insurmountable challenges to the current gold standard treatment for bone repair. The primary purpose of a tissue‐engineered scaffold is to use engineering principles to incite and promote the natural healing process of bone which does not occur in critical‐sized defects. A synthetic bone scaffold must be biocompatible, biodegradable to allow native tissue integration, and mimic the multidimensional hierarchical structure of native bone. In addition to being physically and chemically biomimetic, an ideal scaffold is capable of eluting bioactive molecules (e.g., BMPs, TGF‐βs, etc., to accelerate extracellular matrix production and tissue integration) or drugs (e.g., antibiotics, cisplatin, etc., to prevent undesired biological response such as sepsis or cancer recurrence) in a temporally and spatially controlled manner. Various biomaterials including ceramics, metals, polymers, and composites have been investigated for their potential as bone scaffold materials. However, due to their tunable physiochemical properties, biocompatibility, and controllable biodegradability, polymers have emerged as the principal material in bone tissue engineering. This article briefly reviews the physiological and anatomical characteristics of native bone, describes key technologies in mimicking the physical and chemical environment of bone using synthetic materials, and provides an overview of local drug delivery as it pertains to bone tissue engineering is included. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009

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Section: Physical Effectors In Synthetic Bone Scaffoldsmentioning

confidence: 99%

Bone tissue engineering: A review in bone biomimetics and drug delivery strategies

Porter

Ruckh

Popat

2009

Biotechnology Progress

672

499

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“…As advances in tissue engineering lead to development of new fabrics to be used as cell scaffolds (Li et al, 2002;Mo et al, 2004;Smith and Ma, 2004;Kwon et al, 2005), the potential to regulate cell anchorage to these scaffolds through mechanical entanglement should become an important design element.…”

Section: Discussionmentioning

confidence: 99%

Cell–Matrix Entanglement and Mechanical Anchorage of Fibroblasts in Three-dimensional Collagen Matrices

Jiang

Grinnell²

2005

MBoC

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Fibroblast-3D collagen matrix culture provides a physiologically relevant model to study cell-matrix interactions. In tissues, fibroblasts are phagocytic cells, and in culture, they have been shown to ingest both fibronectin and collagencoated latex particles. Compared with cells on collagen-coated coverslips, phagocytosis of fibronectin-coated beads by fibroblasts in collagen matrices was found to be reduced. This decrease could not be explained by integrin reorganization, tight binding of fibronectin beads to the collagen matrix, or differences in overall bead binding to the cells. Rather, entanglement of cellular dendritic extensions with collagen fibrils seemed to interfere with the ability of the extensions to interact with the beads. Moreover, once these extensions became entangled in the matrix, cells developed an integrin-independent component of adhesion. We suggest that cell-matrix entanglement represents a novel mechanism of cell anchorage that uniquely depends on the three-dimensional character of the matrix. INTRODUCTIONCompared with collagen-coated material surfaces such as plastic or glass, fibroblasts exhibit unique features when they interact with three-dimensional collagen matrices (Cukierman et al., 2002;Grinnell, 2003). The stiffness of fibroblasts is similar to that of three-dimensional (3D) collagen fibrils in the matrix (Wakatsuki et al., 2000). As a result, the interaction between cells and the matrix can lead not only to changes in cell shape but also to matrix remodeling and contraction (Grinnell, 1994;Brown et al., 1998;Tranquillo, 1999;Petroll and Ma, 2003;Vanni et al., 2003;Wakatsuki and Elson, 2003). By contrast, the difference in stiffness between cells and rigid material surfaces such as glass and plastic is so great (Wakatsuki et al., 2000;Callister, 2001) that cell shape change predominates even if the material surface has been modified to make it somewhat flexible (Balaban et al., 2001;Beningo and Wang, 2002).Fibroblasts interact with and attach to collagen matrices primarily through integrin ␣2␤1 but also with other integrins, including ␣1␤1, ␣11␤1, and ␣v␤3 (Klein et al., 1991;Schiro et al., 1991;Carver et al., 1995;Cooke et al., 2000;Tiger et al., 2001;Jokinen et al., 2004). Attached cells, rather than forming flattened, lamellar extensions as occurs on impenetrable glass or plastic surfaces, range in shape from dendritic to stellate to bipolar, depending on matrix stiffness and tension (Grinnell, 2003). Similar morphological features have been described for cells in tissues (Breathnach, 1978;Doljanski, 2004;Goldsmith et al., 2004;Langevin et al., 2005). In part, differences in morphology between cells in twodimensional (2D) versus 3D culture may result from the symmetric adhesive interactions in 3D matrices versus the forced asymmetry of 2D surfaces (Beningo et al., 2004).In tissues, fibroblasts are phagocytic cells (McGaw and Ten Cate, 1983;Everts et al., 1996Everts et al., , 2003, and in culture they have been shown to ingest both fibronectin (FN) and collagen-coated late...

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“…Beyond above-discussed simple units, PA molecules are known to self assemble into sheets, spheres, rods, disks or channels depending on the shape, charge, and environment in which they are placed [70,71]. Stupp and coworkers have developed nano-fiber forming PAs, having five structural features [72].…”

Section: Self-assemblymentioning

confidence: 99%

Biomimetic materials for tissue engineering

Peter

2008

Advanced Drug Delivery Reviews

1,201

819

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Tissue engineering and regenerative medicine is an exciting research area that aims at regenerative alternatives to harvested tissues for transplantation. Biomaterials play a pivotal role as scaffolds to provide three-dimensional templates and synthetic extracellular-matrix environments for tissue regeneration. It is often beneficial for the scaffolds to mimic certain advantageous characteristics of the natural extracellular matrix, or developmental or would healing programs. This article reviews current biomimetic materials approaches in tissue engineering. These include synthesis to achieve certain compositions or properties similar to those of the extracellular matrix, novel processing technologies to achieve structural features mimicking the extracellular matrix on various levels, approaches to emulate cell-extracellular matrix interactions, and biologic delivery strategies to recapitulate a signaling cascade or developmental/would-healing program. The article also provides examples of enhanced cellular/tissue functions and regenerative outcomes, demonstrating the excitement and significance of the biomimetic materials for tissue engineering and regeneration.

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Nano-fibrous scaffolds for tissue engineering

Cited by 591 publications

References 38 publications

Bone tissue engineering: A review in bone biomimetics and drug delivery strategies

Bone tissue engineering: A review in bone biomimetics and drug delivery strategies

Cell–Matrix Entanglement and Mechanical Anchorage of Fibroblasts in Three-dimensional Collagen Matrices

Biomimetic materials for tissue engineering

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