Nano‐fibrous scaffolding architecture selectively enhances protein adsorption contributing to cell attachment

Woo, Kyung Mi; Chen, Victor; Peter, X.

doi:10.1002/jbm.a.10098

Cited by 647 publications

(474 citation statements)

References 21 publications

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“…Synthetic biodegradable polymers such as PCL, unlike natural ECM components, do not have specific cell-binding sides. Thus cell adhesion to pure synthetic polymers is poor and requires additional modifications such as adsorbing ECM proteins onto the polymer surface [48][49][50][51]. In our experiments, no additional surface modifications were necessary facilitating cell attachment onto the fibers of the hybrid scaffolds and both electrospun collagen/elastin/PCL and gelatin/PCL supported attachment and proliferation of hASCs.…”

Section: Discussionmentioning

confidence: 73%

Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

et al. 2008

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Electrospinning using natural proteins or synthetic polymers is a promising technique for the fabrication of fibrous scaffolds for various tissue engineering applications. However, one limitation of scaffolds electrospun from natural proteins is the need to cross-link with glutaraldehyde for stability, which has been postulated to lead to many complications in vivo including graft failure. In this study, we determined the characteristics of hybrid scaffolds composed of natural proteins including collagen and elastin, as well as gelatin, and the synthetic polymer poly(ε-caprolactone) (PCL), so to avoid chemical cross-linking. Fiber size increased proportionally with increasing protein and polymer concentrations, whereas pore size decreased. Electrospun gelatin/PCL scaffolds showed a higher tensile strength when compared to collagen/elastin/PCL constructs. To determine the effects of pore size on cell attachment and migration, both hybrid scaffolds were seeded with adipose-derived stem cells. Scanning electron microscopy and nuclei staining of cell-seeded scaffolds demonstrated complete cell attachment to the surfaces of both hybrid scaffolds, although cell migration into the scaffold was predominantly seen in the gelatin/PCL hybrid. The combination of natural proteins and synthetic polymers to create electrospun fibrous structures resulted in scaffolds with favorable mechanical and biological properties.

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Section: Discussionmentioning

confidence: 73%

Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

et al. 2008

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“…Cells attached on nano-fibrous scaffolds at a level of 70% higher than that on solid-walled scaffolds [99]. The nano-fibrous architecture also enhanced the osteoblastic marker genes' expression ( Figure 5), indicating increased differentiation of the osteoblastic cells [100].…”

Section: Biological Effects Of Nano-fibrous Architecturementioning

confidence: 96%

“…The nano-fibrous scaffolds were found to adsorb 4.2-fold more human serum proteins than solid-walled scaffolds (control scaffolds with smooth pore wall morphology) [99]. Moreover, the profile of serum proteins adsorbed to the nanofibrous scaffold was different from that adsorbed to the solid-walled scaffolds (Figure 4).…”

Section: Biological Effects Of Nano-fibrous Architecturementioning

confidence: 97%

“…While the amount of one protein (arrow) absorbed to both types of scaffolds similarly, some other proteins (arrow heads) exclusively adsorbed onto the nano-fibrous scaffold. Furthermore, Western blot analysis showed that the nano-fibrous scaffolds adsorbed significant levels of fibronectin and vitronectin from serum, while these cell-adhesion proteins were barely detectable on the solid-walled scaffolds [99].…”

Section: Biological Effects Of Nano-fibrous Architecturementioning

confidence: 99%

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Biomimetic materials for tissue engineering

Peter

2008

Advanced Drug Delivery Reviews

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1,201

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Tissue engineering and regenerative medicine is an exciting research area that aims at regenerative alternatives to harvested tissues for transplantation. Biomaterials play a pivotal role as scaffolds to provide three-dimensional templates and synthetic extracellular-matrix environments for tissue regeneration. It is often beneficial for the scaffolds to mimic certain advantageous characteristics of the natural extracellular matrix, or developmental or would healing programs. This article reviews current biomimetic materials approaches in tissue engineering. These include synthesis to achieve certain compositions or properties similar to those of the extracellular matrix, novel processing technologies to achieve structural features mimicking the extracellular matrix on various levels, approaches to emulate cell-extracellular matrix interactions, and biologic delivery strategies to recapitulate a signaling cascade or developmental/would-healing program. The article also provides examples of enhanced cellular/tissue functions and regenerative outcomes, demonstrating the excitement and significance of the biomimetic materials for tissue engineering and regeneration.

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“…Several cells types, including osteoblasts 78,79 , fibroblasts 80,81 , rat kidney cells 80 , smooth muscle cells 82 , neural stem cells 83 and embryonic stem cells 84 , have shown increased attachment on various nano-fibers compared to their corresponding control materials. Additionally, a recent study found that branched nano-fibers improve fibroblast attachment over linear nano-fibers 85 .…”

Section: Attachment and Proliferationmentioning

confidence: 99%

Tissue engineering with nano-fibrous scaffolds

2008

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Tissue Engineering is a rapidly evolving field in terms of cell source and scaffold fabrication. As the template for three dimensional tissue growth, the scaffold should emulate the native extracellular matrix, which is nano-fibrous. Currently, there are three basic techniques capable of generating nanofibrous scaffolding: electrospinning, molecular self-assembly, and thermally induced phase separation. These scaffolds can then be further modified by various three dimensional surface modification techniques if necessary to more precisely emulate the native extracellular matrix. However, even without further modification, nano-fibrous scaffolds have been shown to have advantageous effects on cellular behavior and tissue formation when compared to more traditional types of scaffolding. This review focuses on the current state of tissue engineering with nano-fibrous scaffolding with particular emphasis on bone tissue engineering.

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Nano‐fibrous scaffolding architecture selectively enhances protein adsorption contributing to cell attachment

Cited by 647 publications

References 21 publications

Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

Biomimetic materials for tissue engineering

Tissue engineering with nano-fibrous scaffolds

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