Naja kaouthia venom protein, Nk-CRISP, upregulates inflammatory gene expression in human macrophages

Deka, Archana; Sharma, Manoj Kumar; Mukhopadhyay, Rupak; Devi, Arpita; Doley, Robin

doi:10.1016/j.ijbiomac.2020.05.169

Cited by 13 publications

(10 citation statements)

References 60 publications

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“…The use of the minimum dose of 1.25 ng/mL resulted in decreased survival in both lines within 24 h. The confirmation of the induction of the cytotoxic effect is the high concentration of NOx recorded for both lines. This effect was previously noted for some elapid venoms [58,59]. This indicates that the 3FTx fraction induces inflammation; however, the production of NOx in response to the action of 3FTxs is not the major differentiating factor for the studied cell types.…”

Section: Discussionsupporting

confidence: 70%

Effects of 3FTx Protein Fraction from Naja ashei Venom on the Model and Native Membranes: Recognition and Implications for the Mechanisms of Toxicity

et al. 2021

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Three-finger toxins are naturally occurring proteins in Elapidae snake venoms. Nowadays, they are gaining popularity because of their therapeutic potential. On the other hand, these proteins may cause undesirable reactions inside the body′s cells. A full assessment of the safety of Naja ashei venom components for human cell application is still unknown. The aim of the study was to determine the effect of the exogenous application of three-finger toxins on the cells of monocytes (U-937) and promyelocytes (HL-60), with particular emphasis on the modification of their membranes under the influence of various doses of 3FTx protein fraction (0–120 ng/mL). The fraction exhibiting the highest proportion of 3FTx proteins after size exclusion chromatography (SEC) separation was used in the experiments. The structural response of cell membranes was described on the basis of single-component and multi-component Langmuir monolayers that mimicked the native membranes. The results show that the mechanism of protein–lipid interactions depends on both the presence of lipid polar parts (especially zwitterionic type of lipids) and the degree of membrane saturation (the greatest-for unsaturated lipids). The biochemical indicators reflecting the tested cells (MDA, LDH, cell survival, induction of inflammation, LD50) proved the results that were obtained for the model.

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Section: Discussionsupporting

confidence: 70%

Effects of 3FTx Protein Fraction from Naja ashei Venom on the Model and Native Membranes: Recognition and Implications for the Mechanisms of Toxicity

et al. 2021

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“…Since CRISP can promote smooth muscle contraction, it is presumed that CRISP may act on the circulatory system, to raise blood pressure and promote increased hemorrhage. The silica molecular docking study of cobra CRISP in complex with TLR4-MD2 receptor shows that CRISP is involved in its cysteine-rich structural (CRD) interacting with the complex [48]. The rich diversity of CRISP in intra-and inter-species snake venoms suggests functional diversity.…”

Section: Ontogenetic Variation Predominates In the Snake Venom Of Cap...mentioning

confidence: 99%

Venom Variation of Neonate and Adult Chinese Cobras in Captivity Concerning Their Foraging Strategies

Nie

Chen

Wang

et al. 2022

Toxins

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The venom and transcriptome profile of the captive Chinese cobra (Naja atra) is not characterized until now. Here, LC-MS/MS and illumine technology were used to unveil the venom and trascriptome of neonates and adults N. atra specimens. In captive Chinese cobra, 98 co-existing transcripts for venom-related proteins was contained. A total of 127 proteins belong to 21 protein families were found in the profile of venom. The main components of snake venom were three finger toxins (3-FTx), snake venom metalloproteinase (SVMP), cysteine-rich secretory protein (CRISP), cobra venom factor (CVF), and phosphodiesterase (PDE). During the ontogenesis of captive Chinese cobra, the rearrangement of snake venom composition occurred and with obscure gender difference. CVF, 3-FTx, PDE, phospholipase A2 (PLA2) in adults were more abundant than neonates, while SVMP and CRISP in the neonates was richer than the adults. Ontogenetic changes in the proteome of Chinese cobra venom reveals different strategies for handling prey. The levels of different types of toxin families were dramatically altered in the wild and captive specimens. Therefore, we speculate that the captive process could reshape the snake venom composition vigorously. The clear comprehension of the composition of Chinese cobra venom facilitates the understanding of the mechanism of snakebite intoxication and guides the preparation and administration of traditional antivenom and next-generation drugs for snakebite.

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“…In addition, other protein families are capable of triggering the inflammatory response, such as hyaluronidases and svCRiSPs. However, their role in innate immunity modulation during snakebites has yet to be thoroughly studied ( Bickler, 2020 ; Deka et al, 2020 ). svCRiSPs are a widely distributed family of non-enzymatic proteins with a molecular weight of 24–28 kDa isolated from numerous animals, including reptile venoms ( Matsunaga et al, 2009 ).…”

Section: Introductionmentioning

confidence: 99%

“…svCRiSPs are a widely distributed family of non-enzymatic proteins with a molecular weight of 24–28 kDa isolated from numerous animals, including reptile venoms ( Matsunaga et al, 2009 ). Despite being widely recognized as ion channel blockers, increasing data have shown that svCRiSPs can display other biological properties such as pro-inflammatory effects by inducing cell activation, cytokine release, chemotaxis, and increase of vascular permeability ( Wang et al, 2010 ; Lodovicho et al, 2017 ; Bernardes et al, 2019 ; Suntravat et al, 2019 ; Deka et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Snake venom cysteine-rich secretory protein from Mojave rattlesnake venom (Css-CRiSP) induces acute inflammatory responses on different experimental models

Salazar,

Cirilo,

Reyes

et al. 2024

Toxicon: X

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Naja kaouthia venom protein, Nk-CRISP, upregulates inflammatory gene expression in human macrophages

Cited by 13 publications

References 60 publications

Effects of 3FTx Protein Fraction from Naja ashei Venom on the Model and Native Membranes: Recognition and Implications for the Mechanisms of Toxicity

Effects of 3FTx Protein Fraction from Naja ashei Venom on the Model and Native Membranes: Recognition and Implications for the Mechanisms of Toxicity

Venom Variation of Neonate and Adult Chinese Cobras in Captivity Concerning Their Foraging Strategies

Snake venom cysteine-rich secretory protein from Mojave rattlesnake venom (Css-CRiSP) induces acute inflammatory responses on different experimental models

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