Na+-Ca2+ exchanger modulates Ca2+ content in intracellular Ca2+ stores in rat osteoblasts

Jung, Sang Yong; Park, Yong Joo; Park, Young Jun; Ho, Seok; Lee, Myung Za; Suh, Chang Kook

doi:10.1038/emm.2007.50

Cited by 8 publications

(4 citation statements)

References 40 publications

(39 reference statements)

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“…of 0.27 ± 0.08 was observed, thus indicating a role for NCX in not only mediating calcium influx from extracellular space, but also in stimulating calcium release from intracellular stores. This supports previous observations in rat osteoblasts where a decrease in ATP-dependent Ca 2+ release from intracellular store was observed upon the inhibition of NCX using antisense oligonucleotides (Jung et al 2007).…”

Section: Discussionsupporting

confidence: 92%

The effects of REV5901 on intracellular calcium signalling in freshly isolated bovine articular chondrocytes

Qusous

Parker²,

Ali³

et al. 2012

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REV5901 is an inhibitor of regulatory volume decrease (RVD) a mechanotransduction pathway regulating cell volume in response to hypotonicity, with protective properties upon chondrocyte trauma impact in situ. As the mechanism of action of REV5901 is unknown and changes in intracellular calcium ([Ca2+]i) have been linked to REV5901-loading, we investigated the effects of REV5901 on a known calcium signalling pathway. Upon REV5901 loading, there was significant increase in [Ca2+]i reaching 37.97 ± 5.67%, above basal levels which was reduced to 27.86 ± 3.15% in the presence of 2 mmol/l EGTA. In the presence of U73122 or neomycin there was a decrease in calcium with inhibition factors (I.F.) of 0.39 ± 0.09 and 0.37 ± 0.08, respectively, whereas rottlerin abolished the REV5901-induced [Ca2+]i rise. The role of calcium channels in contributing to the REV5901-induced calcium rise was investigated whereby the calcium rise was inhibited in the absence of extracellular sodium and by the addition of Gd3+ and Ruthenium red. These data show a phospholipase Cβ3-dependent release of calcium from intracellular stores as well as a sodium calcium exchanger-mediated influx in response to REV5901 loading, suggesting a potential role for calcium signalling in mediating the action of REV5901 in chondrocytes.

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Section: Discussionsupporting

confidence: 92%

The effects of REV5901 on intracellular calcium signalling in freshly isolated bovine articular chondrocytes

Qusous

Parker²,

Ali³

et al. 2012

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“…The Na + /Ca 2+ exchanger (NCX) mediates Ca 2+ flowing into the cells mainly through reverse exchange, 66 and plays its role on the surface of osteoblasts, regulates the concentration of Ca 2+ in osteoblasts and promote bone matrix mineralization. 57 , 67 …”

Section: Ca 2+ /Can/nfat Signalling Pathway In Osteoblastogenesismentioning

confidence: 99%

The role of Ca²⁺/Calcineurin/NFAT signalling pathway in osteoblastogenesis

et al. 2021

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The bone remodelling process is closely related to bone health. Osteoblasts and osteoclasts participate in the bone remodelling process under the regulation of various factors inside and outside. Excessive activation of osteoclasts or lack of function of osteoblasts will cause occurrence and development of multiple bone‐related diseases. Ca2+/Calcineurin/NFAT signalling pathway regulates the growth and development of many types of cells, such as cardiomyocyte differentiation, angiogenesis, chondrogenesis, myogenesis, bone development and regeneration, etc. Some evidences indicate that this signalling pathway plays an extremely important role in bone formation and bone pathophysiologic changes. This review discusses the role of Ca2+/Calcineurin/NFAT signalling pathway in the process of osteogenic differentiation, as well as the influence of regulating each component in this signalling pathway on the differentiation and function of osteoblasts, whereby the relationship between Ca2+/Calcineurin/NFAT signalling pathway and osteoblastogenesis could be deeper understood.

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“…In osteoblasts, NCXs are positioned along their surface and adjacent to the site of bone formation (Lundquist, Lundgren, Gritli‐Linde, & Linde, ; Stains, Weber, & Gay, ). They can regulate Ca 2+ transport and content in osteoblasts and bone fluid to facilitate mass mineralization of the bone matrix (Jung et al, ). In tooth‐derived cells, NCXs have been identified in human gingival fibroblasts (Hattori & Wang, ) and rat ameloblasts (Okumura et al, ).…”

Section: Ca2+ Sensors–transporters and Intracellular Ca2+ Signalsmentioning

confidence: 99%

The emerging role of extracellular Ca²⁺ in osteo/odontogenic differentiation and the involvement of intracellular Ca²⁺ signaling: From osteoblastic cells to dental pulp cells and odontoblasts

2018

Journal Cellular Physiology

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Calcium ions (Ca ) is the main element of dental pulp capping materials. Ca signaling plays a crucial role in a myriad of cell activities. An overwhelming array of studies have already reported the experimental and clinical benefits of Ca -enriched materials in the treatment of teeth with accidental vital pulp exposure and incomplete root formation. Thus, Ca signaling has always been an excellent target for the design of various novel biomaterials for use in revitalizing or regenerative endodontic procedures. However, the molecular mechanisms that enable dental pulp cells (DPCs) to detect and respond to extracellular Ca have not been characterized in detail before. In this review, we mainly outline the pathways by which the cell detects and responds to extracellular Ca , as well as the relevant regulatory paths in DPCs and odontoblasts, and discuss the potential role of Ca as a therapeutic tool. Moreover, because DPCs share many of the same functional properties that are found in osteoblasts, some comparisons with bone cells were additionally incorporated into this text.

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Na+-Ca2+ exchanger modulates Ca2+ content in intracellular Ca2+ stores in rat osteoblasts

Cited by 8 publications

References 40 publications

The effects of REV5901 on intracellular calcium signalling in freshly isolated bovine articular chondrocytes

The effects of REV5901 on intracellular calcium signalling in freshly isolated bovine articular chondrocytes

The role of Ca²⁺/Calcineurin/NFAT signalling pathway in osteoblastogenesis

The emerging role of extracellular Ca²⁺ in osteo/odontogenic differentiation and the involvement of intracellular Ca²⁺ signaling: From osteoblastic cells to dental pulp cells and odontoblasts

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Na+-Ca2+ exchanger modulates Ca2+ content in intracellular Ca2+ stores in rat osteoblasts

Cited by 8 publications

References 40 publications

The effects of REV5901 on intracellular calcium signalling in freshly isolated bovine articular chondrocytes

The effects of REV5901 on intracellular calcium signalling in freshly isolated bovine articular chondrocytes

The role of Ca2+/Calcineurin/NFAT signalling pathway in osteoblastogenesis

The emerging role of extracellular Ca2+ in osteo/odontogenic differentiation and the involvement of intracellular Ca2+ signaling: From osteoblastic cells to dental pulp cells and odontoblasts

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Product

Resources

About

The effects of REV5901 on intracellular calcium signalling in freshly isolated bovine articular chondrocytes

The effects of REV5901 on intracellular calcium signalling in freshly isolated bovine articular chondrocytes

The role of Ca²⁺/Calcineurin/NFAT signalling pathway in osteoblastogenesis

The emerging role of extracellular Ca²⁺ in osteo/odontogenic differentiation and the involvement of intracellular Ca²⁺ signaling: From osteoblastic cells to dental pulp cells and odontoblasts