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Küçük, Semra; Çoban, Deniz

doi:10.4194/1303-2712-v16_3_13

Cited by 8 publications

(1 citation statement)

References 28 publications

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“…Although the general recommended dose range of MS-222 is usually broad; in practice, an optimal dose that is effective and safe for a given species under a specific environmental condition should be preferred. The reported optimal doses of MS-222 under a specified rearing condition are 70–90 µg/mL for Asian seabass ( Lates calcarifer ) [ 16 ], 75 µg/mL for fathead minnow ( Pimephales promelas ) [ 17 ] and Senegalese sole ( Solea senegalensis ) [ 18 ], 90–110 µg/mL for yellow catfish ( Tachysurus fulvidraco ) [ 19 ], 100 µg/mL for silver seabream ( Pagrus auratus ) [ 20 ], 100–200 µg/mL for lumpfish ( Cyclopterus lumpus ) [ 21 ], and 300 µg/mL for Nile tilapia ( Oreochromis niloticus ) [ 22 ] and goldfish ( Carassius auratus ) [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

The Use of Tricaine Methanesulfonate (MS-222) in Asian Seabass (Lates calcarifer) at Different Temperatures: Study of Optimal Doses, Minimum Effective Concentration, Blood Biochemistry, Immersion Pharmacokinetics, and Tissue Distributions

Hsu,

Rairat,

et al. 2023

Veterinary Sciences

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This study was conducted to determine the optimal doses and minimum effective concentrations (MECs) of tricaine methanesulfonate (MS-222) in marketable-size Asian seabass reared at two temperatures (22 and 28 °C). Serum biochemical parameters, pharmacokinetics, and tissue distributions of MS-222 following immersion at the determined optimal doses were also evaluated in order to delineate possible mechanisms dictating the temperature difference. The definition of optimal dose is set as the dose when fish attain stage III anesthesia within 5 min, sustain this stage for 3 min, and re-attain equilibrium within 5 min. The MEC is the fish serum MS-222 concentration when stage III anesthesia is reached. The results showed that water temperature exerted no or minimal impact on the designated parameters. The optimal doses at 22 and 28 °C were 140 and 150 µg/mL, while the MECs were 70.48 and 78.27 µg/mL, respectively. Fish exposed to the optimal doses of MS-222 had significantly elevated blood concentrations of lactate, glucose, calcium, magnesium, and sodium, while the blood pH was significantly decreased. The fish eliminated MS-222 faster at 28 °C than at 22 °C, with serum half-lives of 18.43 and 37.01 h, respectively. Tissue-specific distribution patterns were evident. Irrespective of water temperature, MS-222 peaked at 5 min for the brain and gill but peaked slightly later at 10–20 min for the liver and kidney. Most tissues exhibit a gradual decline of drug concentration except for the gill, which was maintained at a steady level. Muscle is the least perfused tissue with the lowest drug concentration throughout the 90 min period. This study provided physiological and pharmacokinetic evidence contributing to a better understanding of the actions of MS-222 in Asian seabass at different temperatures.

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Section: Introductionmentioning

confidence: 99%

The Use of Tricaine Methanesulfonate (MS-222) in Asian Seabass (Lates calcarifer) at Different Temperatures: Study of Optimal Doses, Minimum Effective Concentration, Blood Biochemistry, Immersion Pharmacokinetics, and Tissue Distributions

Hsu,

Rairat,

et al. 2023

Veterinary Sciences

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Sedative Efficacy of Tobacco Extract, Clove Oil, Tricaine Methanesulfonate, and Ketamine: Effects on Hematological Parameters and Blood Biochemical Profile in Sterlet

et al. 2019

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This study was carried out to evaluate the efficacy of four sedatives, including tobacco extract, clove oil, tricaine methanesulfonate (MS‐222), and ketamine, in juvenile Sterlet Acipenser ruthenus by use of two experiments. First, fish with an average weight (±SD) of 64.1 ± 3.4 g were exposed to immersion at six concentrations of each sedative, including clove oil at 14, 35, 56, 77, 98, and 119 mg/L; MS‐222 at 45, 50, 55, 60, 65, and 70 mg/L; ketamine at 35, 37.5, 40, 42.5, 45, and 50 mg/L; and tobacco extract at 375, 450, 525, 600, 675, and 700 mg/L. Induction time (deep induction) and recovery time were measured, and the concentrations inducing lower induction time and recovery (i.e., optimum concentrations) of each sedative were obtained as follows: 56 mg/L for clove oil, 60 mg/L for MS‐222, 45 mg/L for ketamine, and 675 mg/L for tobacco extract. To evaluate the impact of the selected concentration of each sedative on physiological responses of Sterlet, the hematological indices, plasma cortisol, glucose, and lactate concentrations were measured. Additionally, one nonexposed group was considered as a control group. Significant changes were revealed in all hematological parameters except for mean corpuscular hemoglobin concentration and monocytes percent. Biochemical parameters were measured at 0, 1, 3, and 6 h after induction. Significant changes were found in cortisol and lactate levels for all treatments except clove oil. Glucose concentration changed significantly only in the control group. Given that fish sedated with clove oil did not indicate changes in blood chemistry, clove oil may be the more effective sedative according to our results.

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Evaluation of the application of seaweed (Eucheuma cottonii) extract as fish anesthetic agent

et al. 2021

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Cited by 8 publications

References 28 publications

The Use of Tricaine Methanesulfonate (MS-222) in Asian Seabass (Lates calcarifer) at Different Temperatures: Study of Optimal Doses, Minimum Effective Concentration, Blood Biochemistry, Immersion Pharmacokinetics, and Tissue Distributions

The Use of Tricaine Methanesulfonate (MS-222) in Asian Seabass (Lates calcarifer) at Different Temperatures: Study of Optimal Doses, Minimum Effective Concentration, Blood Biochemistry, Immersion Pharmacokinetics, and Tissue Distributions

Sedative Efficacy of Tobacco Extract, Clove Oil, Tricaine Methanesulfonate, and Ketamine: Effects on Hematological Parameters and Blood Biochemical Profile in Sterlet

Evaluation of the application of seaweed (Eucheuma cottonii) extract as fish anesthetic agent

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