N-myc downstream-regulated gene 1/Cap43 expression promotes cell differentiation of human osteosarcoma cells

Matsugaki, Toru; Zenmyo, Michihisa; Hiraoka, Koji; Fukushima, Nobuhiro; Shoda, Takanori; Komiya, Setsuro; Ono, Masahiro; Kuwano, Michihiko; Nagata, Kensei

doi:10.3892/or_00000913

Cited by 10 publications

(2 citation statements)

References 23 publications

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“…NDRG1 is a protein with diverse functions, including regulation of cell growth, apoptosis, cell cycle progression, promotion of tumor cell proliferation, facilitation of tumor invasion, and metastasis. [24][25][26][27] NDRG1 plays a significant role in tumor development as it is involved in the regulation of various signaling pathways, such as the PI3K/ AKT/mTOR pathway, the RAS pathway, the TGFβ pathway, and the Wnt/β-catenin pathways. [28][29][30] Our study provides theoretical support at the single-cell sequencing level for NDRG1 as a potential therapeutic target for breast cancer.…”

Section: Expression Assessment Of the Association Between Vm Risk Mod...mentioning

confidence: 99%

Vasculogenic mimicry regulates immune infiltration and mutational status of the tumor microenvironment in breast cancer to influence tumor prognosis

Zheng,

Guo,

Yang

et al. 2024

Environmental Toxicology

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BackgroundVasculogenic mimicry (VM) refers to the direct formation of microcirculatory ducts by invasive malignant tumors via cellular phenotypic transformation. However, there is a lack of VM‐based biomarkers for breast cancer.MethodsWe obtained transcriptomic expression data, single cell sequencing data, and clinical data of patients from The Cancer Genome Atlas Program (TCGA) database and GEO database, performed single cell analysis to obtain specific type annotations of breast cancer cells and analyzed their spatial expression analysis. Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene ontology (GO) analyses as well as Gene Set Enrichment Analysis (GSEA) analyses were performed to clarify the biological pathways and tumor functional enrichment relationships of the major expressed genes of VM in the breast cancer bulk data specimens. VM biomarkers were constructed. Meanwhile, the relationship between VM scores and tumor immune infiltration in breast cancer was analyzed using MCPcounter and ssGSEA methods. In addition, we assessed the specific relationship between NDRG1, a key VM gene in breast cancer, and tumor colonization, adhesion and invasion by biological experiments in breast cancer cell lines.ResultsThe main cell types of breast cancer (BRCA) samples were annotated by single cell transcriptome analysis. Most of the VM‐high group was present in epithelial cells, whereas the VM‐low group was present in immune and stromal cells. Multiple tumor pathways such as TGFβ p53 and MAPK were closely associated with VM‐mediated breast cancer infiltration and invasion. A prognostic model of breast cancer based on VM key genes was constituted. Prognostic stratification of breast cancer was successfully achieved for the TCGA‐BRCA and GSE58812 datasets. Through immune infiltration analysis, we found that differential expression of VM markers was associated with multiple immune cell regulation. In MDA‐MB‐231 and MDA‐MB‐453 cell lines, we found that the NDRG1 gene significantly promoted colony formation of breast cancer cells.ConclusionOur constructed VM‐related gene‐based model of breast cancer biology holds promise for prognostic prediction and patient stratification of breast cancer. This may provide a potentially clinically valuable aid in promoting a deeper understanding of the biological regulation of VM in breast cancer and exploring the specific mechanisms of tumor angiogenesis and breast cancer development.

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Section: Expression Assessment Of the Association Between Vm Risk Mod...mentioning

confidence: 99%

Vasculogenic mimicry regulates immune infiltration and mutational status of the tumor microenvironment in breast cancer to influence tumor prognosis

Zheng,

Guo,

Yang

et al. 2024

Environmental Toxicology

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“…(2) 5% CMC; or (3) 7.5% HPMC and 2.5% PVP as described by Dannhorn et al, (2020). For the embedding procedure, media (1) and ( 2) were incubated at 50°C to form a liquid solution. Media (3) was preconditioned on ice for 30 min before use.…”

Section: Sample Embeddingmentioning

confidence: 99%

Imaging Three-Dimensional Cell-Culture Models for Pre-Clinical Biopharmaceutical Testing

Flint¹

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Mass spectrometry imaging (MSI) is a multiplex methodology that has the capability to map molecular distributions within biological tissues via an unlabelled approach. The ability of MSI to detect a drug, its active metabolites and the molecular changes to treatment has demonstrated value for pre-clinical efficacy and toxicity studies in the pharmaceutical industry. Parallel to the developments in MSI, three-dimensional (3D) cell cultures have emerged as biologically relevant models for pre-clinical therapeutic research in part as an attempt to address the economic and societal issues of the use of animals in science. The combination of MSI with 3D cell culture models has previously been shown to provide an elegant method for small molecule drug analysis; however, with recent progress in the development of biopharmaceuticals there are increasing demands for advancements in this field.

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NDRG1 Controls Gastric Cancer Migration and Invasion through Regulating MMP-9

Chang

Xue

et al. 2016

Pathol. Oncol. Res.

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The purpose of this study is to detect the clinical significance of NDRG1 and its relationship with MMP-9 in gastric cancer metastatic progression. 101 cases of gastric cancer specimens were utilized to identify the protein expression of NDRG1 and MMP-9 by immunohistochemistry, their clinical significance was also analyzed. The suppression by siRNA-NDRG1 was employed to detect the role of NDRG1 in gastric cancer progression and its relationship with MMP-9. NDRG1 expression was correlated inversely with the degree of tumor cell differentiation (p < 0.01), invasion depth (p < 0.05), lymph node metastasis (p < 0.05) and TNM stage (p < 0.05), whereas MMP-9 was positive correlated with the degree of tumor cell differentiation (p < 0.01), lymph node metastasis (p < 0.05) and TNM stage (p < 0.05), but not correlated with invasion depth (p>0.05). Furthermore, cell proliferation and invasion effect were remarkably enhanced when NDRG1 was silencing, but MMP-9 expression was increased. NDRG1 silencing enhances gastric cancer cells progression through upregulating MMP-9. It suggests that NDRG1 may inhibit the metastasis of gastric cancer via regulating MMP-9.

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N-myc downstream-regulated gene 1/Cap43 expression promotes cell differentiation of human osteosarcoma cells

Cited by 10 publications

References 23 publications

Vasculogenic mimicry regulates immune infiltration and mutational status of the tumor microenvironment in breast cancer to influence tumor prognosis

Vasculogenic mimicry regulates immune infiltration and mutational status of the tumor microenvironment in breast cancer to influence tumor prognosis

Imaging Three-Dimensional Cell-Culture Models for Pre-Clinical Biopharmaceutical Testing

NDRG1 Controls Gastric Cancer Migration and Invasion through Regulating MMP-9

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