N-Acyl Homoserine Lactone Production by Klebsiella pneumoniae Isolated from Human Tongue Surface

Abstract

Bacteria communicate by producing quorum sensing molecules called autoinducers, which include autoinducer-1, an N-hexanoyl homoserine lactone (AHL), and autoinducer-2. Bacteria present in the human oral cavity have been shown to produce autoinducer-2, but not AHL. Here, we report the isolation of two AHL-producing Klebsiella pneumoniae strains from the posterior dorsal surface of the tongue of a healthy individual. Spent culture supernatant extracts from K. pneumoniae activated the biosensors Agrobacterium tumefaciens NTL4(pZLR4) and Escherichia coli [pSB401], suggesting the presence of both long and short chain AHLs. High resolution mass spectrometry analyses of these extracts confirmed that both K. pneumoniae isolates produced N-octanoylhomoserine lactone and N-3-dodecanoyl-l-homoserine lactone. To the best of our knowledge, this is the first report of the isolation of K. pneumoniae from the posterior dorsal surface of the human tongue and the production of these AHLs by this bacterium.

Quorum quenching activity of Syzygium cumini (L.) Skeels and its anthocyanin malvidin against Klebsiella pneumoniae

“…Upon screening K. pneumoniae, (KF817575) showed consistent induction of violacein production in reporter strain indicating its ability to produce AHL. Results obtained in this study is comparable with those of Yin et al [29] who reported the production of both short and long chain AHLs in K. pneumoniae. Limitations were there in screening the spoilage microflora for their signal molecule production as C. violaceum CECT5999 responds only to short range of AHLs [30].…”

Long Chain N-acyl Homoserine Lactone Production by Enterobacter sp. Isolated from Human Tongue Surfaces

“…Recently, we have isolated two AHL-producing K. pneumoniae strains from the posterior dorsal surface of the tongue of a healthy individual [17]. Here we report the isolation of Enterobacter sp.…”

Pseudomonas cremoricolorata Strain ND07 Produces N-acyl Homoserine Lactones as Quorum Sensing Molecules

“…Mass spectrometry parameters and mobile phases used were essentially performed according to a reported method [20]. Precursor ion-scanning analysis were performed in positive ion mode with Q3 set to monitor for m / z 102 and Q1 set to scan a mass range of m / z 80 to 400.…”