N-acetyl cysteine protects human oral keratinocytes from Bis-GMA-induced apoptosis and cell cycle arrest by inhibiting reactive oxygen species-mediated mitochondrial dysfunction and the PI3K/Akt pathway

Zhu, Yu; Gu, Yun; Mo, Jia-Ji; Shi, Junyu; Qiao, Shuqing; Lai, Hong-Chang

doi:10.1016/j.tiv.2015.09.002

Cited by 15 publications

(10 citation statements)

References 81 publications

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“…Experiment protocol was performed as described previously [27,28]. Total RNA samples were extracted with TriPure Isolation Reagent (Roche, Switzerland) and cDNA prepared from 1 mg of total RNA using the SuperScript III System (Invitrogen Life Technologies).…”

Section: Reverse Transcriptase Polymerase Chain Reaction (Rt-pcr)mentioning

confidence: 99%

Stabilization of Slug by NF-κB is Essential for TNF-α -Induced Migration and Epithelial-Mesenchymal Transition in Head and Neck Squamous Cell Carcinoma Cells

Liu

Shi

Wang

et al. 2018

Cell Physiol Biochem

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Background/Aims: Slug protein, a transcription factor for the induction of epithelial-mesenchymal transition (EMT) and cancer cell invasion and metastasis, is frequently upregulated in human epithelial cancers. However, mutation of this gene in cancer is rare, and the mechanism of its dysregulation remains unknown, especially in head and neck squamous cell carcinoma (HNSCC). Methods: We examined the role of TNF-α in the stabilization of Slug by immunoprecipitation-westernblot analysis. Migration of HNSCC cells with or without knockdown of Slug gene expression was assayed by a wound healing assay. Immunohistochemical staining analysis was used to measurement Slug levels in both normal and HNSCC tumor tissues. Results: The inflammatory cytokine TNF-α stabilized Slug protein by inhibiting its ubiquitination through the NF-κB pathway. Inhibition of NF-κB or knockdown of p65 abrogated the TNF-α-induced stabilization of Slug. Knockdown of Slug expression inhibited cancer cell migration and EMT characteristics induced by TNF-α. Moreover, increased levels of Slug were found to correlate with lymph node metastasis and predict poor prognosis in patients with HNSCC. Conclusions: NF-κB-mediated stabilization of Slug underlies the inflammation-induced EMT and metastasis in HNSCC, which may serve as a therapeutic target for metastatic HNSCC.

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Section: Reverse Transcriptase Polymerase Chain Reaction (Rt-pcr)mentioning

confidence: 99%

Stabilization of Slug by NF-κB is Essential for TNF-α -Induced Migration and Epithelial-Mesenchymal Transition in Head and Neck Squamous Cell Carcinoma Cells

Liu

Shi

Wang

et al. 2018

Cell Physiol Biochem

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“…6 N-acetyl cysteine (NAC) is a water-soluble and membrane-permeable small molecule with various intra-and extracellular functionalities, such as antioxidant potential, ability to enhance cytocompatibility and enhance osteogenic differentiation. [7][8][9] NAC is cheaper, safer, and easier to synthesize and deliver than other growth factors, such as BMP-2, and lacks any dose-dependent side effects. 10 A scaffolding system that comprises NAC loaded on a collagenous sponge scaffold has been shown to promote bone regeneration in vitro and in vivo by accelerating osteogenesis.…”

Section: Introductionmentioning

confidence: 99%

<p>NAC-loaded electrospun scaffolding system with dual compartments for the osteogenesis of rBMSCs in vitro</p>

Zhu¹,

Song²,

Ju³

et al. 2019

IJN

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PurposeIn this study, we aimed to develop a unique N-acetyl cysteine (NAC)-loaded polylactic-co-glycolic acid (PLGA) electrospun system with separate compartments for the promotion of osteogenesis.Materials and methodsWe first prepared solutions of NAC-loaded mesoporous silica nanoparticles (MSNs), PLGA, and NAC in N, N-dimethylformamide and tetrahydrofuran for the construction of the electrospun system. We then fed solutions to a specific injector for electrospinning. The physical and chemical properties of the scaffold were characterized through scanning electron microscopy, transmission electron microscopy, and Fourier transform infrared spectroscopy. The release of NAC and Si from different PLGA scaffolds was estimated. The cell viability, cell growth, and osteogenic potential of rat bone marrow-derived stroma cell (rBMSCs) on different PLGA scaffolds were evaluated through MTT assay, live/dead staining, phalloidin staining, and Alizarin red staining. The expression levels of osteogenic-related markers were analyzed through real-time PCR (qRT-PCR).ResultsNAC was successfully loaded into MSNs. The addition of MSNs and NAC decreased the diameters of the electrospun fibers, increased the hydrophilicity and mechanical property of the PLGA scaffold. The release kinetic curve indicated that NAC was released from (PLGA + NAC)/(NAC@MSN) in a biphasic pattern, that featured an initial burst release stage and a later sustained release stage. This release pattern of NAC encapsulated on the (PLGA + NAC)/(NAC@MSN) scaffolds enabled to prolong the high concentrations of release of NAC, thus drastically affecting the osteogenic differentiation of rBMSCs.ConclusionA PLGA electrospun scaffold was developed, and MSNs were used as separate nanocarriers for recharging NAC concentration, demonstrating the promising use of (PLGA + NAC)/(NAC@MSN) for bone tissue engineering.

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“…For real‐time PCR analysis, at 6 and 12 h after seeding, total RNA was extracted using Trizol reagent (Invitrogen). Afterwards, 1 μg total RNA was reverse transcribed into cDNA using PrimeScript 1st Strand cDNA Synthesis kit (TaKaRa, Japan) following established protocols . Then, PCR was carried out using a cDNA equivalent of 10 ng total RNA from each sample with the primers specific for fibronectin 1 (Fn1), Vinculin (VCL), Integrin α5 (ITGA5) and Integrin β1 (ITGB1) and β‐actin.…”

Section: Methodsmentioning

confidence: 99%

“…Specifically, the reaction was carried out in 20 μL of the mixture using SYBR ® Premix Ex Taq™ (TaKaRa, Japan) according to the manufacturer's instructions. The gene expression of each sample was monitored via a Bio‐Rad qRT‐PCR system (Bio‐Rad) and the relative ratios were calculated by the comparative ΔCt method . Here, the housekeeping gene β‐actin was used for normalization.…”

Section: Methodsmentioning

confidence: 99%

Bacterial and mammalian cells adhesion to tantalum‐decorated micro‐/nano‐structured titanium

Zhu

Qiao

et al. 2016

J Biomedical Materials Res

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Microorganisms are frequently introduced to dental implants during surgery and start the race for the surface with host cells before osseointegration occurs. The aim of the study was to endow implant surfaces with biological functions that reliably select cells over microbes. Nano-structured tantalum (Ta) has exhibited excellent compatibility. Thus, nano-structured Ta films were deposited on the sand-blasted, large grit, and acid-etched (SLA) titanium by the magnetron sputtering method, thus forming hierarchical micro-/nano-structured surfaces. No obvious Ta release confirmed the robustness of the deposited layer probably arising from the stable Ta O . Moreover, Ta-modified surfaces not only improved the initial adhesion and spreading of rat bone mesenchymal stem cells (rBMSCs), but also exhibited good antibacterial activities towards Streptococcus mutans and Porphyromonas gingivalis. The satisfactory cell-surface interactions on Ta-modified surfaces depended largely on the up-regulation of adhesion-related genes and activation of focal adhesion kinase (FAK), as confirmed by real-time PCR and Western blot. Here, the coculture model was also forwarded to mimic the perioperative bacterial contamination. We found that the adherent cell number and the cell-surface coverage were hampered by bacteria presence on both surfaces. Yet, rBMSCs still attached and spread more readily on Ta-modified surfaces than on SLA titanium surfaces even in coculture with adhering oral pathogens. Our results revealed that Ta-modified micro-/nano-structured surfaces would selectively promote cell-surface rather than bacteria-surface interactions, boding well for the applications for dental implants in possibly infected environments. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 871-878, 2017.

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N-acetyl cysteine protects human oral keratinocytes from Bis-GMA-induced apoptosis and cell cycle arrest by inhibiting reactive oxygen species-mediated mitochondrial dysfunction and the PI3K/Akt pathway

Cited by 15 publications

References 81 publications

Stabilization of Slug by NF-κB is Essential for TNF-α -Induced Migration and Epithelial-Mesenchymal Transition in Head and Neck Squamous Cell Carcinoma Cells

Stabilization of Slug by NF-κB is Essential for TNF-α -Induced Migration and Epithelial-Mesenchymal Transition in Head and Neck Squamous Cell Carcinoma Cells

<p>NAC-loaded electrospun scaffolding system with dual compartments for the osteogenesis of rBMSCs in vitro</p>

Bacterial and mammalian cells adhesion to tantalum‐decorated micro‐/nano‐structured titanium

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