Myosin isoform transitions in regeneration of fast and slow muscles during postnatal development of the rat

d’Albis, Anne; Couteaux, R; Janmot, Chantal; Mira, Jean‐Claude

doi:10.1016/0012-1606(89)90182-6

Cited by 49 publications

(24 citation statements)

References 14 publications

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“…During skeletal muscle regeneration, MyHC expression recapitulates the events observed during development (12). For rats, it has been established that during muscle regeneration, embryonic and neonatal myosin are expressed before the appearance of adult fast myosins (11).…”

mentioning

confidence: 80%

Expression of slow myosin heavy chain during muscle regeneration is not always dependent on muscle innervation and calcineurin phosphatase activity

Launay¹,

Noirez²,

Butler-Browne³

et al. 2006

American Journal of Physiology-Regulatory, Integrative and Comp

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Launay, Thierry, Philippe Noirez, Gillian Butler-Browne, and Onnik Agbulut. Expression of slow myosin heavy chain during muscle regeneration is not always dependent on muscle innervation and calcineurin phosphatase activity. Am J Physiol Regul Integr Comp Physiol 290: R1508 -R1514, 2006. First published January 19, 2006 doi:10.1152/ajpregu.00486.2005.-In the literature, there is an ambiguity as to the respective roles played by calcineurin phosphatase activity (CPA) and muscle innervation in the reestablishment of the slow-twitch muscle phenotype after muscle regeneration in different species. In this study, we wanted to determine the role of calcineurin and muscle innervation on the appearance and maintenance of the slow phenotype during mouse muscle regeneration. The pattern of myosin expression and CPA was analyzed in adult (n ϭ 15), regenerating (n ϭ 45) and denervated-regenerating (n ϭ 32) slow-twitch soleus and fast-twitch extensor digitorum longus (EDL) muscles. Moreover, in a second group of denervated-regenerating mice (n ϭ 9), the animals were treated with a calcineurin inhibitor. Regeneration was induced by injection of cardiotoxin and in the denervatedregenerating group, denervation was carried out by cutting the sciatic nerve before the administration of cardiotoxin. In innervated-regenerating soleus muscle, CPA increased continuously after 10 days postinjury and by 21 days, there was a 3.5-fold increase in CPA compared with adult basal level, whereas in innervated-regenerating EDL muscle, CPA remained unchanged. Moreover, our results show that in denervated-regenerating muscles, the MyHC profile was identical in spite of the functional differences inherent in these muscles. In long-term denervated-regenerating muscles, a slow muscle phenotype was reexpressed both in the presence or absence of calcineurin inhibitor. Our results show that although in innervated-regenerating mouse muscle, the appearance of a slow phenotype is correlated with a peak of CPA, in denervated-regenerating muscles, a slow phenotype is triggered and maintained in a calcineurin-and nerve-independent manner. myosin heavy chain; cardiotoxin; denervation; nerve injury; immunohistochemistry

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mentioning

confidence: 80%

Expression of slow myosin heavy chain during muscle regeneration is not always dependent on muscle innervation and calcineurin phosphatase activity

Launay¹,

Noirez²,

Butler-Browne³

et al. 2006

American Journal of Physiology-Regulatory, Integrative and Comp

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“…Embryos, cryosections, and satellite cells were fixed with 0.2% glutaraldehyde, permeabilized with 0.01% sodium deoxycholate-0.02% Nonidet P-40, and stained with 5-bromo-4-chloro-3-indolyl-␤-D-galactopyranoside (X-Gal) at 0.5 mg/ml-10 mM K 3 [Fe(CN) 6 ]-10 mM K 4 [Fe(CN) 6 ]. In some cases, sections were counterstained with eosin.…”

Section: Methodsmentioning

confidence: 99%

“…5C). Injection of cardiotoxin is an established model for induction of lesions in skeletal muscle and its regeneration (4,7). In order to compare the regenerative capacities of homozygous and heterozygous Pop1-LacZ mice, cardiotoxin was injected into the hind limbs and muscle tissue was isolated 6 and 12 h and 1, 2, 5, 10, and 20 days later.…”

Section: Vol 22 2002mentioning

confidence: 99%

Mouse Pop1 Is Required for Muscle Regeneration in Adult Skeletal Muscle

Andrée

Fleige

Arnold

et al. 2002

Molecular and Cellular Biology

133

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Popeye (Pop) genes are a novel gene family encoding putative transmembrane proteins predominantly present in striated and smooth muscle cells. In this study, a null mutation of Pop1 was generated by replacing the first coding exon of the Pop1 gene with the lacZ reporter gene. Homozygous mice lacking Pop1 were fertile and had a normal life span without any apparent phenotype. LacZ staining of tissues of heterozygous and homozygous Pop1-LacZ mice revealed strong expression in embryonic and fetal hearts. Pop1-LacZ was also expressed in the myotome and in myogenic progenitor cells within the limb and in smooth muscle cells of various organs. In the heart, Pop1-LacZ activity was downregulated postnatally in heterozygous mice but not in homozygous mice. Administration of the ␤-adrenergic agonist isoproterenol led to a rapid increase in Pop1-LacZ activity in heterozygotes without induction at the transcriptional level, suggesting stabilization of the protein. No difference, however, was observed between homozygous and heterozygous mice in the ability to develop cardiac hypertrophy in response to isoproterenol. The capacity to regenerate skeletal muscle was tested after cardiotoxin injection into the hind limbs of hetero-and homozygous mice. In activated satellite cells of both genotypes, rapid activation of Pop1-LacZ expression was observed. In heterozygous animals, LacZ activity was only transiently elevated in muscle precursor cells undergoing fusion and in newly formed myotubes. In homozygotes, persistence of LacZ expression and a retarded ability to regenerate skeletal muscle were apparent, suggesting that Pop1 plays a role in muscle regeneration.Popeye (Pop) genes represent a novel gene family encoding proteins with three putative transmembrane domains and no other recognizable domain of known structure (2). In mammals, three family members, Pop1 to -3, have been identified that are differentially expressed in heart and skeletal muscles. At the mRNA level, Pop1 and Pop3 are expressed in both muscle types while Pop2 is expressed predominantly in cardiac muscle (2). In the chick, several Pop1 and Pop3 transcripts have been isolated that are presumably generated by alternative splicing from a single gene (2). In contrast, in mammals, the different Pop transcripts are transcribed from individual genes. In situ hybridization of embryonic chicken and mouse hearts revealed that Pop genes are expressed in the myocardial layer and not in the endocardium or epicardium (2). Pop1 was independently isolated by others and referred to as bves (17). In contrast to the expression pattern observed by in situ hybridization, immunohistochemistry analysis employing antibodies raised against synthetically made portions of the chicken bves protein revealed expression in the proepicardial organ and later in the epicardium and the developing coronary vascular system (17, 25). The reason for the contradictory descriptions of mRNA and protein localization of Pop1/bves is not known. Recently, it was reported that Pop1/bves encodes the protot...

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“…To determine what role myoferlin might play in this fusion process, cardiotoxin was injected into the gastrocnemius of wild-type and myoferlin mice (d 'Albis et al, 1989;Davis et al, 1993). Cardiotoxins are polypeptides of 60-65 amino acids isolated from cobra venom, and cause depolarization and degradation of the plasma membrane.…”

Section: Myoferlin Null Mice Fail To Fully Regenerate Muscle After Inmentioning

confidence: 99%

Normal myoblast fusion requires myoferlin

et al. 2005

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Muscle growth occurs during embryonic development and continues in adult life as regeneration. During embryonic muscle growth and regeneration in mature muscle, singly nucleated myoblasts fuse to each other to form myotubes. In muscle growth, singly nucleated myoblasts can also fuse to existing large,syncytial myofibers as a mechanism of increasing muscle mass without increasing myofiber number. Myoblast fusion requires the alignment and fusion of two apposed lipid bilayers. The repair of muscle plasma membrane disruptions also relies on the fusion of two apposed lipid bilayers. The protein dysferlin, the product of the Limb Girdle Muscular Dystrophy type 2 locus, has been shown to be necessary for efficient, calcium-sensitive,membrane resealing. We now show that the related protein myoferlin is highly expressed in myoblasts undergoing fusion, and is expressed at the site of myoblasts fusing to myotubes. Like dysferlin, we found that myoferlin binds phospholipids in a calcium-sensitive manner that requires the first C2A domain. We generated mice with a null allele of myoferlin. Myoferlin null myoblasts undergo initial fusion events, but they form large myotubes less efficiently in vitro, consistent with a defect in a later stage of myogenesis. In vivo, myoferlin null mice have smaller muscles than controls do, and myoferlin null muscle lacks large diameter myofibers. Additionally, myoferlin null muscle does not regenerate as well as wild-type muscle does, and instead displays a dystrophic phenotype. These data support a role for myoferlin in the maturation of myotubes and the formation of large myotubes that arise from the fusion of myoblasts to multinucleate myotubes.

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Myosin isoform transitions in regeneration of fast and slow muscles during postnatal development of the rat

Cited by 49 publications

References 14 publications

Expression of slow myosin heavy chain during muscle regeneration is not always dependent on muscle innervation and calcineurin phosphatase activity

Expression of slow myosin heavy chain during muscle regeneration is not always dependent on muscle innervation and calcineurin phosphatase activity

Mouse Pop1 Is Required for Muscle Regeneration in Adult Skeletal Muscle

Normal myoblast fusion requires myoferlin

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