Myogenic potential of human alveolar mucosa derived cells

Abstract

Difficulties related to the obtainment of stem/progenitor cells from skeletal muscle tissue make the search for new sources of myogenic cells highly relevant. Alveolar mucosa might be considered as a perspective candidate due to availability and high proliferative capacity of its cells. Human alveolar mucosa cells (AMC) were obtained from gingival biopsy samples collected from 10 healthy donors and cultured up to 10 passages. AMC matched the generally accepted multipotent mesenchymal stromal cells criteria and possess population doubling time, caryotype and immunophenotype stability during long-term cultivation. The single myogenic induction of primary cell cultures resulted in differentiation of AMC into multinucleated myotubes. The myogenic differentiation was associated with expression of skeletal muscle markers: skeletal myosin, skeletal actin, myogenin and MyoD1. Efficiency of myogenic differentiation in AMC cultures was similar to that in skeletal muscle cells. Furthermore, some of differentiated myotubes exhibited contractions in vitro. Our data confirms the sufficiently high myogenic potential and proliferative capacity of AMC and their ability to maintain in vitro proliferation-competent myogenic precursor cells regardless of the passage number.

Multicomponent Non-Woven Fibrous Mats with Balanced Processing and Functional Properties

“…The biocompatibility of the fibrous mats was analyzed using human mesenchymal stromal cells (MSCs) isolated from gingival mucosa, as described in [ 25 ]. The MSC cultural media consisted of Dulbecco’s Modified Eagle’s Medium (DMEM)/F12 (1:1, Biolot, St. Petersburg, Russia) supplemented with 10% fetal calf serum (HyClone, Logan, UT, USA), l-glutamine (5 mg/mL, Gibco, Gaithersburg, MD, USA), insulin–transferrin–sodium selenite (1:100, Biolot, St. Petersburg, Russia), bFGF (20 ng/mL, ProSpec, Rehovot, Israel), and gentamycin (50 μg/mL, Paneco, Moscow, Russia).…”

Characteristics, Classification, and Application of Stem Cells Derived from Human Teeth

“…ABMSCs are found to have sufficiently high proliferative capacity and myogenic potential. ABMSCs are positive expressions of CD29, CD44, CD73, CD90, CD105, CD146, and STRO-1, and negative for CD11b, CD19, CD31, CD34, CD45, and CD324 (Table 1) [107][108][109][110]. Protein arrays were used to identify the contents of ABMSCs and found that ABMSCs expressed IL-6 and MCP-1.…”

A Collagen Basketweave from the Giant Squid Mantle as a Robust Scaffold for Tissue Engineering

“…The biocompatibility and cytotoxicity tests were performed using the primary culture of mesenchymal stromal cells (MSCs) isolated from human gingival mucosa as described in [85]. The cells were cultivated in the medium that contained Dulbecco's Modified Eagle's Medium (DMEM)/F12 (1:1, Biolot, St. Petersburg, Russia), 10% fetal calf serum (HyClone, Logan, UT, USA), L-glutamine (5 mg/mL, Gibco, Gaithersburg, MD, USA), insulin-transferrin-sodium selenite (1:100, Biolot, St. Petersburg, Russia), bFGF (20 ng/mL, ProSpec, Rehovot, Israel), and gentamycin (50 µg/mL, Paneco, Moscow, Russia).…”