Myocardial protection against global ischemia with Krebs-Henseleit buffer-based cardioplegic solution

Минасян, С. М.; Galagudza, M. M.; Dmitriev, Yu. V.; Kurapeev, Dmitry; Власов, Т. Д.

doi:10.1186/1749-8090-8-60

Cited by 22 publications

(15 citation statements)

References 26 publications

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“…Despite significant progress in the development of intraoperative myocardial protection techniques, including both optimization of cardioplegia regimens and implementation of new cardioplegia solutions [ 18 , 19 ], high-risk patients may develop severe myocardial IRI resulting in low cardiac output syndrome, left ventricular stunning, life-threatening ventricular arrhythmias, and perioperative myocardial infarction [ 20 - 22 ]. Considering the results of the numerous experimental studies, IP could be useful in reducing these manifestations of cardiac IRI.…”

Section: Discussionmentioning

confidence: 99%

New technique of local ischemic preconditioning induction without repetitive aortic cross-clamping in cardiac surgery

Kurapeev

Кабанов

Гребенник

et al. 2015

J Cardiothorac Surg

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BackgroundSeveral studies have demonstrated that local ischemic preconditioning can reduce myocardial ischemia–reperfusion injury in cardiac surgery patients; however, preconditioning has not become a standard cardioprotective intervention, primarily because of the increased risk of atheroembolism during repetitive aortic cross-clamping. In the present study, we aimed to describe and validate a novel technique of preconditioning induction.MethodsPatients undergoing coronary artery bypass grafting (12 women and 78 men; mean age, 56 ± 11 years) were randomized into 3 groups: (1) Controls (n = 30), (2) Perfusion (n = 30), and (3) Preconditioning (n = 30). All patients were operated under cardiopulmonary bypass using normothermic blood cardioplegia. Preconditioning was induced by subjecting the hemodynamically unloaded heart to 2 cycles of 3 min of ischemia and 3 min of reperfusion with normokalemic blood prior to cardioplegia. In the Perfusion group, the heart perfusion remained unaffected for 12 min. Troponin I (TnI) levels were analyzed before surgery, and 12, 24, 48 h, and 7 days after surgery. The secondary endpoints included the cardiac index, plasma natriuretic peptide level, and postoperative use of inotropes.ResultsPreconditioning resulted in a significant reduction in the TnI level on the 7th postoperative day only (0.10 ± 0.05 and 0.33 ± 0.88 ng/ml in Preconditioning and Perfusion groups, respectively, P < 0.05). In addition, cardiac index was significantly higher in the Preconditioning group than in the Control and Perfusion groups just after weaning from cardiopulmonary bypass. The number of patients requiring inotropic support with ≥ 2 agents after surgery was significantly lower in the Preconditioning and Perfusion group than in the Control group (P < 0.05). No complications of the procedure were recorded in the Preconditioning group.ConclusionsThe preconditioning procedure described can be performed safely in cardiac surgery patients. The application of this technique of preconditioning was associated with certain benefits, including improved left ventricular function after weaning from cardiopulmonary bypass and a reduced need for inotropic support. However, the infarct-limiting effect of preconditioning in the early postoperative period was not evident. The procedure does not involve repetitive aortic cross-clamping, thus avoiding possible embolic complications.

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Section: Discussionmentioning

confidence: 99%

New technique of local ischemic preconditioning induction without repetitive aortic cross-clamping in cardiac surgery

Kurapeev

Кабанов

Гребенник

et al. 2015

J Cardiothorac Surg

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“…The rats were anesthetized with sodium pentobarbital (60 mg/kg intraperitoneally). Each heart was excised via bilateral thoracotomy and perfused through the ascending aorta with Krebs-Henseleit buffer (consisting of the following [in mmol/L]: NaCl, 118.5; KCl, 4.7; NaHCO 3 , 25; KH 2 PO 4 , 1.2; MgSO 4 , 1.2; glucose, 11; and CaCl 2 , 1.5) at a constant pressure of 85 mm Hg [17]. Perfusion pressure was maintained by gravity by using a water-jacketed double-walled glass column connected to the aortic cannula via a three-way stopcock.…”

Section: Methodsmentioning

confidence: 99%

Effects of tetracycline on myocardial infarct size in obese rats with chemically-induced colitis

et al. 2019

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BackgroundRecent evidence suggests that antibiotic-induced changes in the composition of intestinal microflora, as well as the systemic immunoendocrine effects that result from them, can modulate myocardial tolerance to ischemia-reperfusion injury. The aim of this study was to investigate the effects of tetracycline (TTC) on myocardial infarct size in the isolated hearts obtained from obese rats with chemically-induced colitis (CIC). The association between TTC-induced changes in infarct size and intestinal microbiome composition as well as plasma levels of cytokines and short-chain fatty acids (SCFAs) was also studied.MethodsObesity was induced in Wistar rats by feeding them a high-fat, high-carbohydrate diet for five weeks. A single rectal administration of 3% acetic acid (2 mL) to the rats resulted in CIC. Healthy rats as well as obese rats with CIC received TTC (15 mg daily for 3 days) via gavage. The rats were euthanized, after which isolated heart perfusion with simulated global ischemia and reperfusion was performed. Infarct size was determined histochemically. Lipopolysaccharide (LPS) and cytokine levels in plasma were measured by enzyme-linked immunosorbent assay, whereas SCFA levels in plasma were measured by gas chromatography/mass spectrometry. The intestinal microbiome was analyzed using reverse transcription polymerase chain reaction.ResultsThe treatment with TTC resulted in significant infarct size limitation (50 ± 7 vs. 62 ± 4% for the control mice, p < 0.05) in the hearts from intact animals. However, infarct size was not different between the control rats and the obese rats with CIC. Furthermore, infarct size was significantly larger in TTC-treated obese rats with CIC than it was in the control animals (77 ± 5%, p < 0.05). The concentrations of proinflammatory cytokines and LPS in serum were elevated in the obese rats with CIC. Compared to the control rats, the rats with both obesity and CIC had lower counts of Lactobacillus and Bifidobacterium spp. but higher counts of Escherichia coli. The effects of TTC on infarct size were not associated with specific changes in SCFA levels.ConclusionsTTC reduced infarct size in the healthy rats. However, this effect was reversed in the obese animals with CIC. Additionally, it was associated with specific changes in gut microbiota and significantly elevated levels of cytokines and LPS.

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“…Coronary perfusion volumetric rate was also recorded by measuring venous out ow. Perfusion was performed with modi ed Krebs-Henseleit buffer solution (consisting of the following [in mmol/L]: NaCl, 118.5; KCl, 4.7; NaHCO3, 25; KH2PO4, 1.2; MgSO4, 1.2; glucose, 11; and CaCl2, 1.5) at a constant pressure of 80 mm Hg and temperature +37 С [24]. In this case, the left ventricle contracted in isovolumetric mode due to the fact that the volume of the balloon introduced into its cavity was constant and provided preload at the physiological level (no more than 12 mm Hg).…”

Section: Isolated Heart Perfusion According To Langendorffmentioning

confidence: 99%

Comparative Evaluation of Metformin and Liraglutide Cardioprotective Effect in Rats With Impaired Glucose Tolerance

Симаненкова

Минасян

Karonova

et al. 2020

Preprint

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Background: Impaired glucose tolerance (IGT) increases cardiovascular risk and can enlarge myocardial infarction (MI) incidence and severity. There is lack of information about cardioprotective potential of glucose-lowering drugs in IGT. We aimed to evaluate the sustainability of myocardium to ischemia-reperfusion injury in diabetic and IGT rats and to study cardioprotective action of metformin and liraglutide. Methods: Type 2 diabetes mellitus (DM) and IGT were modelled in Wistar rats by high-fat diet and streptozotocin+nicotinamide. 4 weeks after rats were divided into 4 groups: DM (without treatment), IGT (without treatment), IGT+MET (metformin 200 mg/kg per os once daily 8 weeks), IGT+LIRA (liraglutide 0.06 mg/kg s.c. once daily for 8 weeks). Control and high-fat diet groups were made for comparison. After 8 weeks ischemia-reperfusion injury of isolated hearts was performed. Hemodynamic parameters were evaluated and MI size was measured by staining of myocardium slices in triphenyltetrazolium chloride solution. Blood glucose level was measured during the study.Results: Both IGT and DM led to similar worsening of hemodynamic parameters during ischemia-reperfusion period, in comparison with control group. MI size in IGT (56.76 (51.58; 69.07) %) and DM (57.26 (45.51; 70.08) %) groups was significantly large than in control group (42.98 (33.26; 61.84) %) and did not differ between each other. MI size in high-diet group (56.98 (47.11; 62.83) %) was similarly large as in IGT and DM groups (p>0.05). MI size in IGT+MET (42.11 (38.08; 71.96) %) and IGT+LIRA (42.50 (31.37; 60.40) %) was smaller than in both DM and IGT groups (p<0.05 for multiple comparison). Myocardium damage size did not differ in IGT+MET and IGT+LIRA groups (p>0.05). Only LIRA, but not MET administration to IGT rats led to ischemic contracture reduction. Glycemic control was similar satisfactory in IGT, IGT+MET, IGT+LIRA groups. Conclusions: IGT and DM have similarly pronounced negative influence on hemodynamics and MI size in transient global rat ischemia ex vivo. Obesity development also has negative impact on the myocardial infarct size. Both MET and LIRA have infarct-limiting effect independent on their influence on glucose level. LIRA, but not MET, diminishes ischemic contracture in IGT rats.

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Myocardial protection against global ischemia with Krebs-Henseleit buffer-based cardioplegic solution

Cited by 22 publications

References 26 publications

New technique of local ischemic preconditioning induction without repetitive aortic cross-clamping in cardiac surgery

New technique of local ischemic preconditioning induction without repetitive aortic cross-clamping in cardiac surgery

Effects of tetracycline on myocardial infarct size in obese rats with chemically-induced colitis

Comparative Evaluation of Metformin and Liraglutide Cardioprotective Effect in Rats With Impaired Glucose Tolerance

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