Mycotoxin zearalenone induces AIF- and ROS-mediated cell death through p53- and MAPK-dependent signaling pathways in RAW264.7 macrophages

Yu, Ji‐Yeon; Zheng, Zhong; Son, Young‐Ok; Shi, Xianglin; Jang, Young-Oh; Lee, Jeong-Chae

doi:10.1016/j.tiv.2011.07.002

Cited by 88 publications

(50 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Active P53 induces the expression of death genes including the proapoptotic Bcl-2 family member Bax [69], which is a promoter of apoptosis [70]. Previous study suggested that ZEN induced the release of mitochondrial apoptogenic factors (Bcl-2, Bax) and leads to caspase-independent cell death [18]. The inhibition of apoptosis by ZEN was correlated with altered expression levels of the apoptosis-related modulators Bax and Bcl-2 [71].…”

Section: Discussionmentioning

confidence: 95%

See 1 more Smart Citation

Use of modified halloysite nanotubes in the feed reduces the toxic effects of zearalenone on sow reproduction and piglet development

et al. 2015

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 95%

“…In addition to the reproductive disorders, ZEN is frequently implicated in several genotoxic effects [13,14]. Zearalenone has been reported to induce apoptosis in vivo and in vitro [15][16][17][18]. However, available results concerning the induction of apoptosis after ZEN exposure are controversial [19,20].…”

Section: Introductionmentioning

confidence: 99%

Use of modified halloysite nanotubes in the feed reduces the toxic effects of zearalenone on sow reproduction and piglet development

et al. 2015

View full text Add to dashboard Cite

“…Several studies both in vitro and in vivo reported that ZEN enhanced the formation of reactive oxygen species (ROS) and caused oxidative damage [22]–[25], [62]. Oxidative stress results in damage to cellular structures and has been linked to many diseases [63].…”

Section: Discussionmentioning

confidence: 99%

Toxic Effects of Maternal Zearalenone Exposure on Intestinal Oxidative Stress, Barrier Function, Immunological and Morphological Changes in Rats

et al. 2014

View full text Add to dashboard Cite

The present study was conducted to investigate the effects of maternal zearalenone (ZEN) exposure on the intestine of pregnant Sprague-Dawley (SD) rats and its offspring. Ninety-six pregnant SD rats were randomly divided into four groups and were fed with diets containing ZEN at concentrations of 0.3 mg/kg, 48.5 mg/kg, 97.6 mg/kg or 146.0 mg/kg from gestation days (GD) 1 to 7. All rats were fed with mycotoxin-free diet until their offspring were weaned at three weeks of age. The small intestinal fragments from pregnant rats at GD8, weaned dams and pups were collected and studied for toxic effects of ZEN on antioxidant status, immune response, expression of junction proteins, and morphology. The results showed that ZEN induced oxidative stress, affected the villous structure and reduced the expression of junction proteins claudin-4, occludin and connexin43 (Cx43) in a dose-dependent manner in pregnant rats. Different effects on the expression of cytokines were also observed both in mRNA and protein levels in these pregnant groups. Ingestion of high levels of ZEN caused irreversible damage in weaned dams, such as oxidative stress, decreased villi hight and low expression of junction proteins and cytokines. Decreased expression of jejunal interleukin-8 (IL-8) and increased expression of gastrointestinal glutathione peroxidase (GPx2) mRNA were detected in weaned offspring, indicating long-term damage caused by maternal ZEN. We also found that the Nrf2 expression both in mRNA and protein levels were up-regulated in the ZEN-treated groups of pregnant dams and the high-dose of ZEN group of weaned dams. The data indicate that modulation of Nrf2-mediated pathway is one of mechanism via which ZEN affects gut wall antioxidant and inflammatory responses.

show abstract

“…Despite its lower expression, p38β can potentially perform overlapping roles with p38α, whereas p38γ and p38δ have more restricted expression patterns [45]. Recently, many studies have reported that the inhibition of p38 MAPK phosphorylation could either lead to or explain the beneficial effects of particular compound and proteins on cell apoptosis [46,47,48]. In the present investigation, we focussed on the relationship between RSV and p38 MAPK in the ox-LDL-mediated apoptosis of RAW264.7 macrophages.…”

Section: Discussionmentioning

confidence: 99%

Resveratrol Suppresses Oxidised Low-Density Lipoprotein-Induced Macrophage Apoptosis through Inhibition of Intracellular Reactive Oxygen Species Generation, LOX-1, and the p38 MAPK Pathway

Guo

Liu

et al. 2014

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aim: Resveratrol (RSV) may have therapeutic potential for various diseases. Here we investigated the effect of RSV on oxidised low-density lipoprotein- (ox-LDL) induced apoptosis in RAW264.7 macrophages. Methods: Apoptosis of macrophages following incubation with ox-LDL (with or without RSV pre-treatment) was detected by flow cytometry. Western blotting was used to assess the protein expression of Bax, Bcl-2, and caspase-3 as well as ox-LDL receptor 1 (LOX-1) and p38 mitogen-activated protein kinase (MAPK) phosphorylation. Reactive oxygen species (ROS) generation was evaluated by 2', 7'-dichlorofluorescein diacetate, and JC-1 probe was used to determine the mitochondrial transmembrane potential of cells. Results: Ox-LDL significantly reduced viability and increased the rate of apoptosis (P < 0.05) in RAW264.7 cells. However, pre-treatment with RSV resulted in a remarkable decrease in this apoptotic effect. Moreover, ox-LDL caused the up-regulation of Bax and the down-regulation of Bcl-2, as well as the activation of caspase-3. Expression of LOX-1, phosphorylation of p38 MAPK, and intracellular ROS production also increased after ox-LDL stimulation. Strikingly, these effects were abolished by pre-treatment of cells with RSV. Conclusion: RSV suppresses ox-LDL-induced macrophage apoptosis. These beneficial effects might be exerted through inhibition of ROS generation, LOX-1, and the p38 MAPK signalling pathway.

show abstract

Mycotoxin zearalenone induces AIF- and ROS-mediated cell death through p53- and MAPK-dependent signaling pathways in RAW264.7 macrophages

Cited by 88 publications

References 46 publications

Use of modified halloysite nanotubes in the feed reduces the toxic effects of zearalenone on sow reproduction and piglet development

Use of modified halloysite nanotubes in the feed reduces the toxic effects of zearalenone on sow reproduction and piglet development

Toxic Effects of Maternal Zearalenone Exposure on Intestinal Oxidative Stress, Barrier Function, Immunological and Morphological Changes in Rats

Resveratrol Suppresses Oxidised Low-Density Lipoprotein-Induced Macrophage Apoptosis through Inhibition of Intracellular Reactive Oxygen Species Generation, LOX-1, and the p38 MAPK Pathway

Contact Info

Product

Resources

About