Mycoplasma hyopneumoniae Transcription Unit Organization: Genome Survey and Prediction

Siqueira, Franciele Maboni; Schrank, Augusto; Schrank, Irene Silveira

doi:10.1093/dnares/dsr028

Cited by 12 publications

(31 citation statements)

References 35 publications

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“…It was previously shown that most genes from the genomes of M. hyopneumoniae , M. flocculare and M. hyorhinis are expressed at some basal level and that the majority of the genes are co-transcribed [34, 35]. Therefore, a global determination of the genomic functional elements is a prerequisite to expand our knowledge regarding transcriptional small RNA regulation in swine respiratory mycoplasmas.In the current study, we have analyzed and predicted trans-encoded small RNAs from M. hyopneumoniae , M. flocculare and M. hyorhinis genomes.…”

Section: Introductionmentioning

confidence: 99%

Mycoplasma non-coding RNA: identification of small RNAs and targets

et al. 2016

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BackgroundBacterial non-coding RNAs act by base-pairing as regulatory elements in crucial biological processes. We performed the identification of trans-encoded small RNAs (sRNA) from the genomes of Mycoplama hyopneumoniae, Mycoplasma flocculare and Mycoplasma hyorhinis, which are Mycoplasma species that have been identified in the porcine respiratory system.ResultsA total of 47, 15 and 11 putative sRNAs were predicted in M. hyopneumoniae, M. flocculare and M. hyorhinis, respectively. A comparative genomic analysis revealed the presence of species or lineage specific sRNA candidates. Furthermore, the expression profile of some M. hyopneumoniae sRNAs was determined by a reverse transcription amplification approach, in three different culture conditions. All tested sRNAs were transcribed in at least one condition. A detailed investigation revealed a differential expression profile for two M. hyopneumoniae sRNAs in response to oxidative and heat shock stress conditions, suggesting that their expression is influenced by environmental signals. Moreover, we analyzed sRNA-mRNA hybrids and accessed putative target genes for the novel sRNA candidates. The majority of the sRNAs showed interaction with multiple target genes, some of which could be linked to pathogenesis and cell homeostasis activity.ConclusionThis study contributes to our knowledge of Mycoplasma sRNAs and their response to environmental changes. Furthermore, the mRNA target prediction provides a perspective for the characterization and comprehension of the function of the sRNA regulatory mechanisms.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3061-z) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Mycoplasma non-coding RNA: identification of small RNAs and targets

et al. 2016

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“…Initially, all tandem repeats (SSR, SSRM and DR) found upstream of the first gene of M . hyopneumoniae strain 7448 transcription units [9, 10] were compared against three other mycoplasma genomes: M . hyopneumoniae strain 7422 –pathogenic; M .…”

Section: Methodsmentioning

confidence: 99%

“…Culture conditions, RNA isolation and cDNA synthesis by Reverse Transcription (RT) were performed as described in Siqueira et al [10]. Target and primer descriptions for M .…”

Section: Methodsmentioning

confidence: 99%

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Repetitive Elements in Mycoplasma hyopneumoniae Transcriptional Regulation

et al. 2016

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Transcriptional regulation, a multiple-step process, is still poorly understood in the important pig pathogen Mycoplasma hyopneumoniae. Basic motifs like promoters and terminators have already been described, but no other cis-regulatory elements have been found. DNA repeat sequences have been shown to be an interesting potential source of cis-regulatory elements. In this work, a genome-wide search for tandem and palindromic repetitive elements was performed in the intergenic regions of all coding sequences from M. hyopneumoniae strain 7448. Computational analysis demonstrated the presence of 144 tandem repeats and 1,171 palindromic elements. The DNA repeat sequences were distributed within the 5’ upstream regions of 86% of transcriptional units of M. hyopneumoniae strain 7448. Comparative analysis between distinct repetitive sequences found in related mycoplasma genomes demonstrated different percentages of conservation among pathogenic and nonpathogenic strains. qPCR assays revealed differential expression among genes showing variable numbers of repetitive elements. In addition, repeats found in 206 genes already described to be differentially regulated under different culture conditions of M. hyopneumoniae strain 232 showed almost 80% conservation in relation to M. hyopneumoniae strain 7448 repeats. Altogether, these findings suggest a potential regulatory role of tandem and palindromic DNA repeats in the M. hyopneumoniae transcriptional profile.

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“…Mycoplasma hyopneumoniae is the pathogen with a major contribution to the PRDC [104][105]. The mycoplasmas are prokaryotic pathogens of humans and other animals, distinguished by the lack of a cell wall, diminutive size, and a limited genome.…”

Section: Mycoplasma Hyopneumoniaementioning

confidence: 99%

Porcine Respiratory Disease Complex and Biofilms

Loera‐Muro¹,

Ramírez-Castillo²,

Avelar-González³

et al. 2015

J Bacteriol Parasitol

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The Porcine respiratory disease complex (PRDC) is a term used to describe polymicrobial respiratory infections in pigs. Respiratory diseases in pigs are common in modern pork production worldwide and are the responsible for major economic losses in the swine industry. Pathogens involved in respiratory disease in pigs vary significantly among farms, production sites, regions and countries, making generalizations about the PRDC treatment and difficult to control it. The interactions that occur on the cellular and molecular levels during concurrent infection of pigs with two or more respiratory pathogens are multi-faceted and convoluted. There are a variety of bacterial and viral pathogens commonly associated with the PRDC. The main associated bacteria include: Actinobacillus pleuropneumoniae, Streptococcus suis, Pasteurella multocida, Bordetella bronchiseptica, Haemophilus pasaruis, and Mycoplasma hyopneumoniae. Currently, it is known among microbiologists that biofilm formation is an universal attribute of microorganisms and the main way of life in nature that are causing problems such as developing diseases in animals and humans. Here, is reviewed the current knowledge of the major bacteria involved in this disease, their ability to form biofilms, as well as their importance on the infection process.

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Mycoplasma hyopneumoniae Transcription Unit Organization: Genome Survey and Prediction

Cited by 12 publications

References 35 publications

Mycoplasma non-coding RNA: identification of small RNAs and targets

Mycoplasma non-coding RNA: identification of small RNAs and targets

Repetitive Elements in Mycoplasma hyopneumoniae Transcriptional Regulation

Porcine Respiratory Disease Complex and Biofilms

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