Mycobacterium lacus sp. nov., a novel slowly growing, non-chromogenic clinical isolate.

Turenne, Christine Y.; Chedore, Pamela; Wolfe, Jonathan; Broukhanski, George; May, Kevin; Kabani, Amin

doi:10.1099/00207713-52-6-2135

Cited by 18 publications

(13 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The lipidic pattern, which was not investigated by TLC and GLC, is characterized in HPLC by a single cluster of peaks practically overlapping that of M. gastri (156).…”

Section: Lacusmentioning

confidence: 99%

See 1 more Smart Citation

Impact of Genotypic Studies on Mycobacterial Taxonomy: the New Mycobacteria of the 1990s

2003

View full text Add to dashboard Cite

The advancement of genetic techniques has greatly boosted taxonomic studies in recent years. Within the genus Mycobacterium, 42 new species have been detected since 1990, most of which were grown from clinical samples. Along with species for which relatively large numbers of strains have been reported, some of the new species of mycobacteria have been detected rarely or even only once. From the phenotypic point of view, among the new taxa, chromogens exceed nonchromogens while the numbers of slowly and rapidly growing species are equivalent. Whereas conventional identification tests were usually inconclusive, an important role was played by lipid analyses and in particular by high-performance liquid chromatography. Genotypic investigations based on sequencing of 16S rRNA gene have certainly made the most important contribution. The investigation of genetic relatedness led to the redistribution of the species previously included in the classically known categories of slow and rapid growers into new groupings. Within slow growers, the intermediate branch related to Mycobacterium simiae and the cluster of organisms related to Mycobacterium terrae have been differentiated; among rapid growers, the group of thermotolerant mycobacteria has emerged. The majority of species are resistant to isoniazid and, to a lesser extent, to rifampin. Many of the new species of mycobacteria are potentially pathogenic, and there are numerous reports of their involvement in diseases. Apart from disseminated and localized diseases in immunocompromised patients, the most frequent infections in immunocompetent people involve the lungs, skin, and, in children, cervical lymph nodes. The awareness of such new mycobacteria, far from being a merely speculative exercise, is therefore important for clinicians and microbiologists

show abstract

“…The lipidic pattern, which was not investigated by TLC and GLC, is characterized in HPLC by a single cluster of peaks practically overlapping that of M. gastri (156).…”

Section: Lacusmentioning

confidence: 99%

“…The only strain isolated so far was obtained from the synovial tissue excised from the elbow of a woman with bursitis of posttraumatic origin, and the new species was described in 2002 (156).…”

Section: Lacusmentioning

confidence: 99%

Impact of Genotypic Studies on Mycobacterial Taxonomy: the New Mycobacteria of the 1990s

2003

View full text Add to dashboard Cite

show abstract

“…Sample preparation, PCR, DNA sequencing, as well as editing of the 16S rRNA gene and ITS1 sequences have been described previously (Turenne et al, 2001(Turenne et al, , 2002. Sequence comparisons of both targets were performed using the RIDOM database (Harmsen et al, 2002), in-house data (Turenne et al, 2001) and GenBank sequences at NCBI.…”

mentioning

confidence: 99%

Molecular characterization of nontuberculous mycobacteria isolated from human cases of disseminated disease in the USA, Thailand, Malawi, and Tanzania

Ballard

Turenne

Wolfe

et al. 2007

J. Gen. Appl. Microbiol.

Self Cite

View full text Add to dashboard Cite

“…The lysates were then centrifuged for 2 min at 12 000 g and the supernatant was used for PCR. Amplification of the 16S rRNA gene (Rogall et al, 1990;Turenne et al, 2001) and the ITS1 region (Harmsen et al, 2002;Turenne et al, 2002) was performed as previously described and the PCR products were directly sequenced using forward and reverse primers and loaded on an ABI PRISM 310 Genetic Analyzer capillary sequencer (Applied Biosystems). Sequence editing and phylogenetic analyses by CLUSTAL V (neighbour-joining method) were performed using the SEQMAN and MEGALIGN components of LASERGENE 5 (DNASTAR).…”

mentioning

confidence: 99%

Mycobacterium parascrofulaceum sp. nov., novel slowly growing, scotochromogenic clinical isolates related to Mycobacterium simiae

Turenne

Cook

Burdz

et al. 2004

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

A group of pigmented, slowly growing mycobacteria identified by 16S rRNA gene sequencing as 'MCRO 33' (GenBank accession no. AF152559) have been isolated from several clinical specimens in various laboratories across Canada. Genotypically, the organism is most closely related to Mycobacterium simiae. However, it presents with a similar phenotypic profile to Mycobacterium scrofulaceum. Several reference strains obtained from ATCC and TMC culture collections, previously identified as M. scrofulaceum or M. simiae, have also been found to possess the MCRO 33 16S rRNA gene sequence. Biochemical testing, susceptibility testing, HPLC, hsp65 gene and 16S-23S spacer (ITS1) sequencing were performed on clinical and reference strains to characterize further this unique species. Of the clinical strains, one was isolated from a cervix biopsy whereas all other clinical isolates were obtained from respiratory samples. In one patient, symptoms, imaging and repeat clinical specimens positive on culture for this organism were suggestive of active clinical disease. The description of this species, for which the name Mycobacterium parascrofulaceum sp. nov. is proposed, follows the present trend of a large number of novel Mycobacterium species identified due in great part to sequence-based methods. The type strain is HSC68 T (=ATCC BAA-614 T =DSM 44648 T ). INTRODUCTIONSequence-based identification of non-tuberculous mycobacteria (NTM) has become a method of choice in several mycobacteriology laboratories and is accomplished by analysis of various targets, such as the 16S rRNA gene, 16S-23S spacer region (ITS1) and hsp65 gene. As a result, we are faced with the discovery of many novel species previously undifferentiable using other methods of identification. These cannot be ignored, since current studies show that the detection of novel species is a common occurrence that will only be compounded with an increase in use of automated sequencers in clinical laboratories, with some of these unusual isolates deemed clinically significant (Tortoli et al., 2001). The purpose of this study was to establish as a novel species a group of representative mycobacterial strains commonly found, not only in human clinical isolates obtained from our laboratory, but also in several past publications where evidence of its existence has been found. This species has shown similarities to several known NTM species using single identification methods, which may have been the cause for the delay in its establishment, but it is clearly a unique species as determined from a polyphasic approach. In one symptomatic patient, this organism was isolated in multiple respiratory samples, and associated computed tomography (CT) scan and chest X-ray showed evidence of respiratory disease, as described below.

show abstract

Mycobacterium lacus sp. nov., a novel slowly growing, non-chromogenic clinical isolate.

Cited by 18 publications

References 18 publications

Impact of Genotypic Studies on Mycobacterial Taxonomy: the New Mycobacteria of the 1990s

Impact of Genotypic Studies on Mycobacterial Taxonomy: the New Mycobacteria of the 1990s

Molecular characterization of nontuberculous mycobacteria isolated from human cases of disseminated disease in the USA, Thailand, Malawi, and Tanzania

Mycobacterium parascrofulaceum sp. nov., novel slowly growing, scotochromogenic clinical isolates related to Mycobacterium simiae

Contact Info

Product

Resources

About