Mycobacteria-Specific Cytokine Responses Detect Tuberculosis Infection and Distinguish Latent from Active Tuberculosis

Tebruegge, Marc; Dutta, Binita; Donath, Susan; Ritz, Nicole; Forbes, Benjamin; Camacho-Badilla, Kattia; Clifford, Vanessa; Zufferey, Christel; Robins‐Browne, Roy M.; Hanekom, Willem A.; Graham, Stephen M.; Connell, Tom; Curtis, Nigel

doi:10.1164/rccm.201501-0059oc

Cited by 103 publications

(99 citation statements)

References 49 publications

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“…Our results, showing that 8/15 TST + /QFT -children screened positive with our IP-10 release assay, agree with the concept that combining IFN-γ and IP-10 testing may be one approach to increase the sensitivity of QFT in young children [6]. Our results also agree with previous studies showing an IL-13 response to M.tb peptides in pediatric TB [7][8][9].…”

Section: *Corresponding Authorsupporting

confidence: 91%

Immune response to Mycobacterium tuberculosis in young contacts with discordant immunological test results

Jeljeli

Khourouj

Pommelet

et al. 2016

Journal of Infection

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Section: *Corresponding Authorsupporting

confidence: 91%

Immune response to Mycobacterium tuberculosis in young contacts with discordant immunological test results

Jeljeli

Khourouj

Pommelet

et al. 2016

Journal of Infection

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“…however, IGRAs more often present with indeterminate or false negative results, which is particularly the case for young and immune-compromised children [2,[4][5][6][7]. Identification of novel cytokine biomarkers that can improve the performance of IGRAs (independently or in combination with IFN-γ) has been evaluated by several studies [8][9][10]. These studies demonstrated that additional cytokines and cytokine combinations might contribute to the detection of M. tuberculosis infection and active tuberculosis in children [8,[11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Alternative Quantiferon cytokines for diagnosis of children with active tuberculosis and HIV co-infection in Ghana

Lundtoft

Awuah

Nausch

et al. 2017

Med Microbiol Immunol

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IFN-γ release assays (IGRAs) often present false-negative or indeterminate results in children with tuberculosis. HIV co-infection may contribute to decreased sensitivity of IGRAs by impairing T-cell IFN-γ expression. Measurement of alternative cytokines in QuantiFERON (QFT) supernatants can circumvent the IFN-γ-dependency and may improve QFT sensitivity. We aimed to identify additional cytokines from QFT supernatants for detection of Mycobacterium tuberculosis infection in children with tuberculosis and HIV co-infection from Ghana. Concentrations of 18 cytokines in QFT supernatants from children (0-16 years) with tuberculosis concomitantly infected with HIV (n = 25) or without HIV (n = 24) from Ghana were measured using cytometric bead array (CBA). 29% of the children showed positive IFN-γ test results, and five cytokines, i.e., IL-6, IL-21, TNF-α, IL-1α and IP-10, detected M. tuberculosis infection with comparable or, for IL-6, with significantly higher sensitivity (59%). Increased age and HIV co-infection were associated with decreased cytokine induction, and especially IL-21 and IP-10 were less prevalent in HIV co-infected children with tuberculosis. Combined cytokine analyses increased proportions of positive tests, and a four-cytokine subset (i.e., IL-6, IL-21, IFN-γ, IL-1α) predicted 78% of the children with tuberculosis correctly. Combined evaluation of IFN-γ and alternative cytokines improved IGRA-sensitivity in children with tuberculosis.

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“…A similar approach combining the QGIT, TST, and cytokine profiles was assessed by Tebruegge and colleagues looking at 149 children with possible TB (12). The cytokine profiles assessed included IFN-g, IFN-inducible protein-10 (IP-10), tumor necrosis factora, IL-1ra, IL-2, IL-13, and macrophage inflammatory protein-1b, and the study assessed whether combinations of the cytokine profiles in addition to the results of the TST and QGIT could distinguish between latent and active TB.…”

Section: Diagnosis Of Latent and Active Diseasementioning

confidence: 99%

Update in Tuberculosis/Pulmonary Infections 2015

Koenig

Furin

2016

Am J Respir Crit Care Med

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Mycobacteria-Specific Cytokine Responses Detect Tuberculosis Infection and Distinguish Latent from Active Tuberculosis

Cited by 103 publications

References 49 publications

Immune response to Mycobacterium tuberculosis in young contacts with discordant immunological test results

Immune response to Mycobacterium tuberculosis in young contacts with discordant immunological test results

Alternative Quantiferon cytokines for diagnosis of children with active tuberculosis and HIV co-infection in Ghana

Update in Tuberculosis/Pulmonary Infections 2015

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