Mx1, Mx2 and Mx3 proteins from the gilthead seabream (Sparus aurata) show in vitro antiviral activity against RNA and DNA viruses

Fernández-Trujillo, M.A.; García-Rosado, Esther; Alonso, M. Carmen; Castro, Dolores; Álvarez, M. Carmen; Béjar, Julia

doi:10.1016/j.molimm.2013.06.018

Cited by 42 publications

(38 citation statements)

References 42 publications

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“…Mx proteins have been shown to provide resistance to a wide range of both DNA and RNA viruses in vertebrates (28,29). In this study, the expression of Mx1 was significantly upregulated (P < 0.05) by transfection of the FTH1 plasmid.…”

Section: Discussionmentioning

confidence: 59%

The cSNP scanning and expression analysis of the duck FTH1 gene

Zhang¹,

Tong²,

Yang³

et al. 2017

Turk J Vet Anim Sci

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Materials and methods Ethics statementAll procedures involving the care and use of animals conformed to US National Institutes of Health guidelines Abstract: Ferritin, heavy polypeptide 1 (FTH1) is a kind of ferritin complex that catalyzes the conversion of Fe(II) into Fe(III) to protect the cell from oxidative damage. Recent research has shown that FTH1 is also associated with several disease-related processes, such as inflammation and tumor progression and so on. In this study, the coding sequence (CDS) of FTH1 in Jinding ducks was cloned. The CDS sequence revealed an open reading frame of 546 nucleotides encoding a protein with 181 amino acids. Based on this sequence, the duck FTH1 protein is predicted to have conserved domains typical of eukaryotic ferritin. Only one synonymous mutation (c.447T > C) of the coding sequence of the duck FTH1 gene was identified in ducks. Quantitative RT-PCR results revealed that FTH1 expression in the liver and spleen was significantly downregulated after duck hepatitis virus 1 infection. Meanwhile, in DF-1 cells transfected with an FTH1 plasmid, expression of the antiviral marker gene Mx1 was significantly upregulated. These results suggest that the coding sequence of the duck FTH1 gene was highly conserved and FTH1 plays an important role in antivirus activity.

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Section: Discussionmentioning

confidence: 59%

The cSNP scanning and expression analysis of the duck FTH1 gene

Zhang¹,

Tong²,

Yang³

et al. 2017

Turk J Vet Anim Sci

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show abstract

“…In this study, we saw evidence for induction of IFNα (11-fold upregulated) in wt -infected cells and several ISGs including MXD (9-fold up) and MHC class I (5-fold up), but did not detect upregulation of a piscine homolog of PKR, a cellular product that in most mammalian systems lead to phosphorylation of eukaryotic initiation factor 2α and the global inhibition of protein synthesis (Sadler and Williams, 2008). MX proteins have been shown to inhibit the replication of influenza and other viruses and may play a similar role here (Fernandez-Trujillo et al, 2013; Sadler and Williams, 2008). MX proteins are wide spread among both mammals and lower vertebrates and a putative MX gene has been identified in FHM cells (GenBank AY751300.1).…”

Section: Discussionmentioning

confidence: 90%

Differential transcription of fathead minnow immune-related genes following infection with frog virus 3, an emerging pathogen of ectothermic vertebrates

et al. 2014

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Frog virus 3 (FV3) and other ranaviruses are responsible for die-offs involving wild, farmed, and captive amphibians, fish, and reptiles. To ascertain which elements of the immune system respond to infection, we explored transcriptional responses following infection of fathead minnow cells with either wild type (wt) FV3 or a knock out (KO) mutant targeting the 18 kDa immediate early gene (18K). At 8 hr post infection we observed marked upregulation of multiple transcripts encoding proteins affecting innate and acquired immunity. Sequences expressed 4-fold or higher in wt-infected cells included transcripts encoding interferon (IFN), IFN regulatory factors (IRFs), IFN stimulated genes (ISGs) such as Mx and MHC class I, and interleukins IL-1β, IL-8, IL-17C and IL-12. Cells infected with the 18K KO mutant (Δ18K) showed qualitative differences and lower levels of induction. Collectively, these results indicate that ranavirus infection induced expression of multiple cellular genes affecting both innate and acquired immunity.

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“…Therefore, VHSV antagonistic mechanisms seem to depend on complex interactions between Mx promoters and cell-specific factors, similarly to what it has been proposed for IPNV. Concerning seabream Mx proteins, Mx1 and Mx3 showed antiviral activity against VHSV (Fernández-Trujillo et al, 2013), thus, it is also tempting to associate the resistance of this species to VHSV infection with the apparent absence of antagonizing effect of this virus against gilthead seabream Mx expression. In addition, it would be interesting to investigate the apparent additive effect of poly I:C and VHSV in the induction of pMx2.…”

Section: Discussionmentioning

confidence: 99%