volume 66, issue 8, P4855-4863 1992
DOI: 10.1128/jvi.66.8.4855-4863.1992
View full text
M G Romanelli, P Mavromara-Nazos, D Spector, B Roizman

Abstract: demonstrated that substitution of sequences of the thymidine kinase (tk) gene, a 13 gene, extending from-16 to +51 with sequences extending from-12 to +104 of the 'Y2 UL49.5 gene in viral recombinant R3820 conferred upon the chimeric gene Xy2 attributes in the context of the viral genome in a productive infection. The UL49.5 gene sequences extending from-179 to +104 contain four DNA binding sites for the major regulatory protein ICP4. Of these sites, two map between nucleotides +20 and +80 within the sequence …

expand abstract