Mutation of NtNRAMP3 improves cadmium tolerance and its accumulation in tobacco leaves by regulating the subcellular distribution of cadmium

Jia, Hao; Yin, Zhuoran; Xuan, Dongdong; Lian, Wenli; Han, Dan; Zhu, Zhimin; Li, Chenyi; Li, Chang; Song, Zhiwei

doi:10.1016/j.jhazmat.2022.128701

Cited by 24 publications

(8 citation statements)

References 58 publications

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“…Similarly, overexpression of CjNramp1 in Arabidopsis resulted in high tolerance to Cd ( Table 1 ) [ 57 ]. Furthermore, overexpression of NtNramp1 in tobacco could promote Cd uptake and Fe transportation ( Table 1 ) [ 58 ], and the tonoplast-localized NtNramp3 transporter was found to be involved in the regulation of Cd transport from the vacuole to the cytoplasm using CRISPR/Cas9 technology ( Table 1 ) [ 59 ].…”

Section: Natural Resistance-associated Macrophage Proteinsmentioning

confidence: 99%

Advances in Genes-Encoding Transporters for Cadmium Uptake, Translocation, and Accumulation in Plants

Tao

2022

Toxics

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Cadmium (Cd) is a heavy metal that is highly toxic for plants, animals, and human beings. A better understanding of the mechanisms involved in Cd accumulation in plants is beneficial for developing strategies for either the remediation of Cd-polluted soils using hyperaccumulator plants or preventing excess Cd accumulation in the edible parts of crops and vegetables. As a ubiquitous heavy metal, the transport of Cd in plant cells is suggested to be mediated by transporters for essential elements such as Ca, Zn, K, and Mn. Identification of the genes encoding Cd transporters is important for understanding the mechanisms underlying Cd uptake, translocation, and accumulation in either crop or hyperaccumulator plants. Recent studies have shown that the transporters that mediate the uptake, transport, and accumulation of Cd in plants mainly include members of the natural resistance-associated macrophage protein (Nramp), heavy metal-transporting ATPase (HMA), zinc and iron regulated transporter protein (ZIP), ATP-binding cassette (ABC), and yellow stripe-like (YSL) families. Here, we review the latest advances in the research of these Cd transporters and lay the foundation for a systematic understanding underlying the molecular mechanisms of Cd uptake, transport, and accumulation in plants.

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Section: Natural Resistance-associated Macrophage Proteinsmentioning

confidence: 99%

Advances in Genes-Encoding Transporters for Cadmium Uptake, Translocation, and Accumulation in Plants

Tao

2022

Toxics

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“…Rapid economic and technological developments have resulted in the release of a relatively large amount of heavy metal waste, which has led to increased environmental pollution. In China, more than 1.3 × 10 5 km 2 of cultivated land has been polluted with cadmium (Cd) ( MER and MLR, 2014 ; Jia et al., 2022 ). Soil degradation due to Cd contamination is becoming a major issue affecting food security and quality ( Rehman et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…Phytoremediation has attracted widespread interest as an economically feasible and eco-friendly method for rescuing Cd-contaminated soils ( Hamid et al., 2020 ; Raza et al., 2020 ). The Cd accumulation and detoxification capacities of plants are the main determinants of phytoremediation efficiency ( Wang et al., 2021 ; Jia et al., 2022 ). The remediation of Cd-contaminated soil by hyperaccumulating plants is one of the most promising environmentally friendly remediation methods ( Yaashikaa et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

“…A novel Nicotiana tabacum Cd transporter gene NtNRAMP3 preventing chlorophyll degradation and reducing ROS accumulation under Cd stress conditions. Nevertheless, knockdown of the NtNRAMP3 gene could increase the activities of SOD, POD and CAT thereby improving the Cd tolerance of tobacco to a great degree ( Jia et al., 2022 ). Furthermore, SaCu/Zn SOD expression in S. alfredii is induced by Cd stress; the overexpression of this gene increases the resistance of transgenic A. thaliana plants to oxidative stress ( Li et al., 2017 ).…”

Section: Introductionmentioning

confidence: 99%

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A novel gene SpCTP3 from the hyperaccumulator Sedum plumbizincicola redistributes cadmium and increases its accumulation in transgenic Populus × canescens

Zhuo²,

Yu³

et al. 2023

Front. Plant Sci.

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A cadmium (Cd) tolerance protein (SpCTP3) involved in the Sedum plumbizincicola response to Cd stress was identified. However, the mechanism underlying the Cd detoxification and accumulation mediated by SpCTP3 in plants remains unclear. We compared wild-type (WT) and SpCTP3-overexpressing transgenic poplars in terms of Cd accumulation, physiological indices, and the expression profiles of transporter genes following with 100 μmol/L CdCl2. Compared with the WT, significantly more Cd accumulated in the above-ground and below-ground parts of the SpCTP3-overexpressing lines after 100 μmol/L CdCl2 treatment. The Cd flow rate was significantly higher in the transgenic roots than in the WT roots. The overexpression of SpCTP3 resulted in the subcellular redistribution of Cd, with decreased and increased Cd proportions in the cell wall and the soluble fraction, respectively, in the roots and leaves. Additionally, the accumulation of Cd increased the reactive oxygen species (ROS) content. The activities of three antioxidant enzymes (peroxidase, catalase, and superoxide dismutase) increased significantly in response to Cd stress. The observed increase in the titratable acid content in the cytoplasm might lead to the enhanced chelation of Cd. The genes encoding several transporters related to Cd2+ transport and detoxification were expressed at higher levels in the transgenic poplars than in the WT plants. Our results suggest that overexpressing SpCTP3 in transgenic poplar plants promotes Cd accumulation, modulates Cd distribution and ROS homeostasis, and decreases Cd toxicity via organic acids. In conclusion, genetically modifying plants to overexpress SpCTP3 may be a viable strategy for improving the phytoremediation of Cd-polluted soil.

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“…It also explains why ABA reduces the uptake of Cd in plants ( Zhang et al, 2019 ). Recently Jia et al (2022) , reported that the knockout of NtNRAMP3 increases Cd tolerance by reducing cytosolic Cd accumulation in tobacco. Specific promising targets to improve Cd tolerance by genome editing include miR390 from rice, a negative regulator of Cd tolerance.…”

Section: Introductionmentioning

confidence: 99%

Genome Editing Targets for Improving Nutrient Use Efficiency and Nutrient Stress Adaptation

et al. 2022

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In recent years, the development of RNA-guided genome editing (CRISPR-Cas9 technology) has revolutionized plant genome editing. Under nutrient deficiency conditions, different transcription factors and regulatory gene networks work together to maintain nutrient homeostasis. Improvement in the use efficiency of nitrogen (N), phosphorus (P) and potassium (K) is essential to ensure sustainable yield with enhanced quality and tolerance to stresses. This review outlines potential targets suitable for genome editing for understanding and improving nutrient use (NtUE) efficiency and nutrient stress tolerance. The different genome editing strategies for employing crucial negative and positive regulators are also described. Negative regulators of nutrient signalling are the potential targets for genome editing, that may improve nutrient uptake and stress signalling under resource-poor conditions. The promoter engineering by CRISPR/dead (d) Cas9 (dCas9) cytosine and adenine base editing and prime editing is a successful strategy to generate precise changes. CRISPR/dCas9 system also offers the added advantage of exploiting transcriptional activators/repressors for overexpression of genes of interest in a targeted manner. CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) are variants of CRISPR in which a dCas9 dependent transcription activation or interference is achieved. dCas9-SunTag system can be employed to engineer targeted gene activation and DNA methylation in plants. The development of nutrient use efficient plants through CRISPR-Cas technology will enhance the pace of genetic improvement for nutrient stress tolerance of crops and improve the sustainability of agriculture.

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Mutation of NtNRAMP3 improves cadmium tolerance and its accumulation in tobacco leaves by regulating the subcellular distribution of cadmium

Cited by 24 publications

References 58 publications

Advances in Genes-Encoding Transporters for Cadmium Uptake, Translocation, and Accumulation in Plants

Advances in Genes-Encoding Transporters for Cadmium Uptake, Translocation, and Accumulation in Plants

A novel gene SpCTP3 from the hyperaccumulator Sedum plumbizincicola redistributes cadmium and increases its accumulation in transgenic Populus × canescens

Genome Editing Targets for Improving Nutrient Use Efficiency and Nutrient Stress Adaptation

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