Mutagenicity of some indoles and related compounds in the Ames test

Synthesis and Physicochemical Properties of 2-SF5-(Aza)Indoles, a New Family of SF5 Heterocycles

“…We thus performed the Ames test which is a classical biological test to determine the mutagenic potential of a chemical compound . Since cancers are often linked to damage to DNA, this rapid, reliable, and inexpensive test is used to estimate the carcinogenic and genetic activity at the nucleotide level, based on different histidine-requiring bacterial strains of Salmonella typhimurium carrying mutations in the genes in the absence and presence of a liver-metabolizing system. , Over the years, a large database has been accumulated with this assay, confirming its ability to detect genetically active compounds of most chemical classes with around 80–90% sensitivity and specificity. The 2-SF 5 -indoles 5a and 8 have been tested, and no mutagenic evidence was observed over the different bacterial strains tested, which means that they can potentially be used for further development in drug discovery…”

Synthesis and Physicochemical Properties of 2-SF5-(Aza)Indoles, a New Family of SF5 Heterocycles

“…We thus performed the Ames test which is a classical biological test to determine the mutagenic potential of a chemical compound . Since cancers are often linked to damage to DNA, this rapid, reliable, and inexpensive test is used to estimate the carcinogenic and genetic activity at the nucleotide level, based on different histidine-requiring bacterial strains of Salmonella typhimurium carrying mutations in the genes in the absence and presence of a liver-metabolizing system. , Over the years, a large database has been accumulated with this assay, confirming its ability to detect genetically active compounds of most chemical classes with around 80–90% sensitivity and specificity. The 2-SF 5 -indoles 5a and 8 have been tested, and no mutagenic evidence was observed over the different bacterial strains tested, which means that they can potentially be used for further development in drug discovery…”

Scientific Opinion on Flavouring Group Evaluation 77, Revision 1 (FGE.77Rev1): Consideration of Pyridine, Pyrrole and Quinoline Derivatives evaluated by JECFA (63rd meeting) structurally related to Pyridine, Pyrrole, Indole and Quinoline Derivatives evaluated by EFSA in FGE.24Rev2 (2013)

“…Ames assay S. typhimurium TA98 3 nmol -30 µmol/plate (12 doses) (3935 µg/plate) Negative 1 (Curvall et al, 1982) The study is considered valid.…”

Cytotoxicity of Odorous Compounds from Poultry Manure

“…Since fluorescence is more sensitive than absorbance in this assay, it is the recommended detection method [30]. Fluorescence was measured at 560 nm excitation and 590 nm emission, using a microplate reader.…”

Synthesis and Physicochemical Properties of 2-SF5-(Aza)Indoles, a New Family of SF5 Heterocycles

“…We thus performed the Ames test which is a classical biological test to determine the mutagenic potential of a chemical compound . Since cancers are often linked to damage to DNA, this rapid, reliable, and inexpensive test is used to estimate the carcinogenic and genetic activity at the nucleotide level, based on different histidine-requiring bacterial strains of Salmonella typhimurium carrying mutations in the genes in the absence and presence of a liver-metabolizing system. , Over the years, a large database has been accumulated with this assay, confirming its ability to detect genetically active compounds of most chemical classes with around 80–90% sensitivity and specificity. The 2-SF 5 -indoles 5a and 8 have been tested, and no mutagenic evidence was observed over the different bacterial strains tested, which means that they can potentially be used for further development in drug discovery…”

Scientific Opinion on Flavouring Group Evaluation 77, Revision 1 (FGE.77Rev1): Consideration of Pyridine, Pyrrole and Quinoline Derivatives evaluated by JECFA (63rd meeting) structurally related to Pyridine, Pyrrole, Indole and Quinoline Derivatives evaluated by EFSA in FGE.24Rev2 (2013)

“…Ames assay S. typhimurium TA98 3 nmol -30 µmol/plate (12 doses) (3935 µg/plate) Negative 1 (Curvall et al, 1982) The study is considered valid.…”

Cytotoxicity of Odorous Compounds from Poultry Manure

“…Since fluorescence is more sensitive than absorbance in this assay, it is the recommended detection method [30]. Fluorescence was measured at 560 nm excitation and 590 nm emission, using a microplate reader.…”

Synthesis and Physicochemical Properties of 2-SF5-(Aza)Indoles, a New Family of SF5 Heterocycles

“…We thus performed the Ames test which is a classical biological test to determine the mutagenic potential of a chemical compound . Since cancers are often linked to damage to DNA, this rapid, reliable, and inexpensive test is used to estimate the carcinogenic and genetic activity at the nucleotide level, based on different histidine-requiring bacterial strains of Salmonella typhimurium carrying mutations in the genes in the absence and presence of a liver-metabolizing system. , Over the years, a large database has been accumulated with this assay, confirming its ability to detect genetically active compounds of most chemical classes with around 80–90% sensitivity and specificity. The 2-SF 5 -indoles 5a and 8 have been tested, and no mutagenic evidence was observed over the different bacterial strains tested, which means that they can potentially be used for further development in drug discovery…”

Scientific Opinion on Flavouring Group Evaluation 77, Revision 1 (FGE.77Rev1): Consideration of Pyridine, Pyrrole and Quinoline Derivatives evaluated by JECFA (63rd meeting) structurally related to Pyridine, Pyrrole, Indole and Quinoline Derivatives evaluated by EFSA in FGE.24Rev2 (2013)

“…Ames assay S. typhimurium TA98 3 nmol -30 µmol/plate (12 doses) (3935 µg/plate) Negative 1 (Curvall et al, 1982) The study is considered valid.…”

Cytotoxicity of Odorous Compounds from Poultry Manure

“…Since fluorescence is more sensitive than absorbance in this assay, it is the recommended detection method [30]. Fluorescence was measured at 560 nm excitation and 590 nm emission, using a microplate reader.…”