Mushroom body signaling is required for locomotor activity rhythms in Drosophila

Mabuchi, Ikumi; Shimada, Naoto; Sato, Seisho; Ienaga, Kahori; Inami, Show; Sakai, Tomoaki

doi:10.1016/j.neures.2016.04.005

Cited by 18 publications

(21 citation statements)

References 38 publications

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“…Immunohistochemistry was performed as previously described (Mabuchi et al, 2016; Shimada et al, 2016). For Pdf staining, brains were stained with a mouse anti-Pdf antibody (PDF C7-s, Developmental Studies Hybridoma Bank at the University of Iowa, 1:200), and Alexa Fluor 568 anti-mouse IgG (A11004, Thermo Fisher Scientific) was used as the secondary antibody (1:1000).…”

Section: Methodsmentioning

confidence: 99%

Environmental light is required for maintenance of long-term memory in Drosophila

Inami

Sato

Kondo³

et al. 2019

Preprint

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17 18 Long-term memory (LTM) is stored as functional modifications of relevant neural circuits 19 in the brain. However, it remains poorly understood how the consolidated memory is 20 maintained for a long period. Here, we show that in Drosophila, universally available 21 environmental light plays a critical role in LTM maintenance by reactivating the memory 22 modulator cAMP response element-binding protein (CREB) via neuropeptide signaling. 23 LTM is impaired when flies are kept in constant darkness during the memory maintenance 24 phase. Because light activates the brain neurons expressing the neuropeptide 25 Pigment-dispersing factor (Pdf) through the brain photoreceptors, we examined the 26 possible involvement of Pdf in LTM maintenance and found that temporal knockdown of 27 Pdf during the memory maintenance phase impairs LTM in light-dark cycles. Furthermore, 28 we demonstrated that the Pdf receptor (Pdfr) and CREB are required in the memory center 29 for LTM maintenance and Pdfr regulates light-dependent transcription via CREB.30 3 90When flies were kept in LL after conditioning, their LTM was intact [ Figure 1A; (7)], as previously 91 reported (Sakai et al., 2004). Furthermore, the LTM of several clock mutants except for period (per) 92 5 mutants is intact (Sakai et al., 2004). Thus, light input, but not the circadian clock, is necessary for LTM 93 maintenance. 94 Sleep plays an important role in the consolidation of Drosophila courtship memory (Donlea et al., 95 2011). However, it remains unclear whether an abnormal sleep phenotype is attributable to disturbed 96 LTM maintenance of flies kept in DD. To examine whether light conditions affect Drosophila sleep (see 97 Materials and methods), sleep amount was measured in flies kept in LD and DD (Figure 2A). The mean 98 amount of daytime sleep of flies kept in DD was lower than that of flies kept in LD (Figures 2A-C).99 However, when flies in LD were slightly deprived of sleep to adjust the amount of daytime sleep to the 100 level of that in DD (Figures 2D-F), their LTM maintenance was not attenuated (Figure 2G). Thus, LTM 101 impairment induced by DD does not simply result from the reduced amount of sleep. 102 103 Expression of brain photoreceptors in Pdf neurons is necessary for LTM maintenance in LD 104 Because light directly activates Pdf neurons through circadian photoreceptors [Rhodopsin 7 (Rh7) and 105 Cryptochrome (Cry)] (Fogle et al., 2011; Ni et al., 2017; Sheeba et al., 2008), Pdf neurons may regulate 106 light-driven LTM maintenance. To examine whether Rh7 and Cry are required for LTM, Rh7 and cry null 107 mutant flies (Rh7 1 and cry 01 , respectively) were used. Since memory on d 1 (one day after conditioning) 108 was intact in Rh7 1 and cry 01 flies after 7 h conditioning (Figure 1B), Rh7 and cry are dispensable for 109 memory consolidation. Unlike the memory on d 1, Rh7 1 and cry 01 flies showed memory impairment on d 110 5 ( Figure 1C), suggesting that Rh7 and Cry are required for keeping LTM more than one day. We next 111 sought to determin...

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Section: Methodsmentioning

confidence: 99%

Environmental light is required for maintenance of long-term memory in Drosophila

Inami

Sato

Kondo³

et al. 2019

Preprint

Self Cite

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“…However, like ChT, several other proteins, namely; Drosophila Neurexins, Scribble, adaptor protein DRK and Wallenda have also been reported to have localisation in MB and play a role in maintaining structural plasticity at NMJs via different signalling cascades (Moressis et al, 2009; Rui et al, 2017; Shin and DiAntonio, 2011). Previous reports also describe that MB physiology regulates locomotor activity rhythms in Drosophila (Gorostiza et al, 2014; Mabuchi et al, 2016). Future studies are required to elucidate the potential downstream circuit that links the role of ChT in MB physiology and MB motor output.…”

Section: Discussionmentioning

confidence: 92%

Choline Transporter in α/β core neurons of Drosophila mushroom body non-canonically regulates pupal eclosion and maintains neuromuscular junction integrity

Hamid

Hajirnis

Kushwaha³

et al. 2018

Preprint

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“…In cockroaches, procaine (a reversible voltage dependent sodium channels blocker) injection to the MB increases spontaneous walking (Kaiser and Libersat, 2015). In Drosophila, the MB are also required for daily rhythmic locomotor activity (Mabuchi et al, 2016) and enhance motor activity in the beginning of light-evoked walking (Serway et al, 2009). Hence, all aforementioned experimental evidence suggests that the MB have a regulatory role for walking related locomotion.…”

Section: The Cerebral Ganglia (Crg)mentioning

confidence: 99%

On the Role of the Head Ganglia in Posture and Walking in Insects

et al. 2020

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In insects, locomotion is the result of rhythm generating thoracic circuits and their modulation by sensory reflexes and by inputs from the two head ganglia, the cerebral and the gnathal ganglia (GNG), which act as higher order neuronal centers playing different functions in the initiation, goal-direction, and maintenance of movement. Current knowledge on the various roles of major neuropiles of the cerebral ganglia (CRG), such as mushroom bodies (MB) and the central complex (CX), in particular, are discussed as well as the role of the GNG. Thoracic and head ganglia circuitries are connected by ascending and descending neurons. While less is known about the ascending neurons, recent studies in large insects and Drosophila have begun to unravel the identity of descending neurons and their appropriate roles in posture and locomotion. Descending inputs from the head ganglia are most important in initiating and modulating thoracic central pattern generating circuitries to achieve goal directed locomotion. In addition, the review will also deal with some known monoaminergic descending neurons which affect the motor circuits involved in posture and locomotion. In conclusion, we will present a few issues that have, until today, been little explored. For example, how and which descending neurons are selected to engage a specific motor behavior and how feedback from thoracic circuitry modulate the head ganglia circuitries. The review will discuss results from large insects, mainly locusts, crickets, and stick insects but will mostly focus on cockroaches and the fruit fly, Drosophila.

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Mushroom body signaling is required for locomotor activity rhythms in Drosophila

Cited by 18 publications

References 38 publications

Environmental light is required for maintenance of long-term memory in Drosophila

Environmental light is required for maintenance of long-term memory in Drosophila

Choline Transporter in α/β core neurons of Drosophila mushroom body non-canonically regulates pupal eclosion and maintains neuromuscular junction integrity

On the Role of the Head Ganglia in Posture and Walking in Insects

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