Muscle Gene Expression Patterns in Human Rotator Cuff Pathology

Choo, Alexander D.; McCarthy, Meagan M.; Pichika, Rajeswari; Sato, Eugene; Lieber, Richard L.; Schenk, Simon; Lane, Joseph R.; Ward, Samuel R.

doi:10.2106/jbjs.m.01585

Cited by 30 publications

(36 citation statements)

References 39 publications

(41 reference statements)

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“…It is postulated that increased fibrosis results in higher tension in the muscle in RC tears, thus making repair more challenging in chronic, retracted tears. In a clinical setting, fibrosis has been found in human RC tissue at time of repair and has been correlated with decreased force production and increased disruption of myofibril architecture 6,[22][23][24] . Fibrosis has also been identified as a direct cause of muscle dysfunction in muscular dystrophy.…”

Section: @ C I C E D I Z I O N I I N T E R N a Z I O N A L Imentioning

confidence: 99%

Investigating the cellular origin of rotator cuff muscle fatty infiltration and fibrosis after injury

Liu

Ning

Chang

et al. 2016

MLTJ

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SummaryBackground: rotator cuff muscle atrophy, fibrosis and fatty infiltration are common complications after large and massive rotator cuff tears. Currently, there are no effective treatments for these muscle pathologies after injury. Furthermore, the cellular source for fibrotic and adipose tissues in rotator cuff muscle after injury remains unknown. In this study, we proposed that two groups of muscle resident progenitors, Tie2+ muscle mesenchymal progenitors and PDGFRα + fibro/adipogenic progenitor cells (FAPs), contribute significantly to rotator cuff muscle fibrosis and fatty infiltration. Methods: we tested our hypothesis using reporter mice. Rotator cuff muscles from Tie2-GFP and PDGFRα-GFP reporter mice were harvested at 2 and 6 weeks after unilateral massive rotator cuff tear surgeries. Immunofluorescent staining for fibroblast and adipocyte markers was conducted. Results: our results showed significant co-localization of Tie2+ cells with fibrotic markers vimentin and αSMA. In the PDGFRα-GFP reporter mice, GFP signal was seen in only a small fraction of cells staining positive for vimentin and αSMA. However, PDGFRα showed significant co-localization with adipocyte markers, including PPAR-γ, adiponectin, and perilipin A. Oil red O staining confirmed that the mature adipocytes appearing in rotator cuff muscles after injury are also PDGFRα + . Conclusion: these data demonstrated that the Tie2 + muscle mesenchymal progenitors are the major source of fibroblasts while PDGFRα + FAPs are the major source of adipocytes in rotator cuff muscle fatty infiltration. Basic Science Study.

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Section: @ C I C E D I Z I O N I I N T E R N a Z I O N A L Imentioning

confidence: 99%

Investigating the cellular origin of rotator cuff muscle fatty infiltration and fibrosis after injury

Liu

Ning

Chang

et al. 2016

MLTJ

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“…In clinical and animal studies of rotator cuff tears, tendinous tears at the insertion characterized by pathological fatty infiltration, muscle atrophy, and fibrosis in proximal muscular tissues were observed. 15,16 Therefore, these observations show that tendinous damage partially induces fatty degeneration of proximal muscular tissues.…”

Section: Discussionmentioning

confidence: 74%

Decreased postoperative gluteus medius muscle cross-sectional area measured by computed tomography scan in patients with intertrochanteric fractures nailing

Noda

Saegusa

Takahashi

et al. 2017

Journal of Orthopaedic Surgery

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Background: In patients with femoral intertrochanteric fractures treated by cephalomedullary (CM) nailing, abduction force reportedly decreased by 25-30% during the postoperative follow-up period. The purpose of the current study is to evaluate the cross-sectional area (CSA) and adipose tissue ratio (ATR) of the gluteus medius muscle on the postoperative computed tomography (CT) view, expecting this graphic study will support clinical results. Materials and methods: A total of 27 patients with femoral intertrochanteric fractures treated by CM femoral nail implants completed the study. The mean age at osteosynthesis was 83 years (range: 72-94 years). The mean postoperative follow-up period was 23 months. The three CT axial slice views were defined as slices A, B, and C corresponding to proximal, midway, and distal part of gluteus medius, respectively. The CSA and ATR were assessed bilaterally. Results: The mean and standard deviation of CSA values (mm 2 ) between the nonoperated/ operated side were as follows: slice A: 2225.8 + 621.2/1984.5 + 425.8; slice B: 2145.1 + 538.3/1854.9 + 383.9; and slice C: 1711.0 + 459.0/1434.5 + 396.9 (p < 0.01 in slices A, B, and C). The mean and standard deviation of ATR values (%) from the nonoperative/ operative side were as follows: slice A: 2.8 + 1.7/ 5.2 + 3.5; slice B: 2.7 + 1.9/4.6 + 3.2; and slice C: 3.6 + 3.0/4.8 + 3.2 (p < 0.01 in slices A and B and p < 0.05 in slice C). Conclusion: Our image findings documented that gluteus medius is significantly changed in CSA and ATR. The damage possibly triggers decrease in muscular strength of hip abduction in the postoperative follow-up period. This measurement is objective, and needed no patient's endurance and cooperation.

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“…14 Interestingly, expression of myogenic and adipogenic genes was variable with regard to injury type and severity. 6 For example, myogenic gene expression was upregulated in patients with bursitis and tendinopathy but down regulated in patients with massive tendon tears, while adipogenic gene expression was upregulated in patients with a full-thickness tear but down regulated in patients with massive tears. Future work should consider using the quantitative metrics described in this study to probe gross differences in intramuscular fat due to these differing patterns of gene expression.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, despite a common insertion tendon for the rotator cuff muscle-tendon units, 31 these muscles have substantially different muscle architecture, 37 which may influence their mechanosensitivity. 6 These muscles also have differing gene expressions, 30 suggesting that other inherent factors may contribute to the differences in fat distribution and clustering in the rotator cuff, which should be explored further.…”

Section: Discussionmentioning

confidence: 99%

Quantitative Analysis of Three-Dimensional Distribution and Clustering of Intramuscular Fat in Muscles of the Rotator Cuff

et al. 2015

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The purpose of this study was to 1) develop and present a technique to quantitatively assess three-dimensional distribution and clustering of intramuscular fat and 2) use the technique to compare spatial characteristics of intramuscular fat in rotator cuff muscles of older adults with and without a supraspinatus tear. Moran’s Index (I), an existing quantitative measure of clustering, was extended for use with MRI to allow comparisons across individuals with different size muscles. Sixteen older adults (>60yrs) with (N=6) and without (N=10) a degenerative supraspinatus tear participated. Following 3D Dixon MRIs of the shoulder, which separates fat from water, rotator cuff muscles were segmented and sectioned and fat% and Moran’s I were calculated to assess distribution and clustering, respectively. Moran’s I ranged was 0.40–0.92 and 0.39–0.76 for the tear and control subjects, respectively. Compared to uninjured controls, tear subjects demonstrated increased fat distribution (p=0.036) and clustering (p=0.020) distally in the supraspinatus. Tear subjects had more pronounced distribution (p<0.001) and clustering distally (p<0.001) than proximally. Other rotator cuff muscles exhibited different patterns of fat clustering and distribution. This technique, which we applied to quantify spatial characteristics of intramuscular fat, can be applied to assess clustering of fat in other pathologies and tissues.

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Muscle Gene Expression Patterns in Human Rotator Cuff Pathology

Abstract: Background: Rotator cuff pathology is a common source of shoulder pain with variable etiology and pathoanatomical

Cited by 30 publications

References 39 publications

Investigating the cellular origin of rotator cuff muscle fatty infiltration and fibrosis after injury

Investigating the cellular origin of rotator cuff muscle fatty infiltration and fibrosis after injury

Decreased postoperative gluteus medius muscle cross-sectional area measured by computed tomography scan in patients with intertrochanteric fractures nailing

Quantitative Analysis of Three-Dimensional Distribution and Clustering of Intramuscular Fat in Muscles of the Rotator Cuff

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