2011
DOI: 10.1371/journal.pone.0016908
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Abstract: Variations in gene promoter/enhancer activity in different muscle fiber types after gene transduction was noticed previously, but poorly analyzed. The murine stem cell virus (MSCV) promoter drives strong, stable gene expression in hematopoietic stem cells and several other cells, including cerebellar Purkinje cells, but it has not been studied in muscle. We injected a lentiviral vector carrying an MSCV-EGFP cassette (LvMSCV-EGFP) into tibialis anterior muscles and observed strong EGFP expression in muscle fibe… Show more

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Cited by 11 publications
(10 citation statements)
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References 52 publications
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“…Cross-sections of 8 μm thick were cut and mounted on Fisher Superfrost Plus slides (Thermo Fisher Scientific). Myosin-ATPase staining was performed as described 37 . Briefly, muscle sections were pre-incubated for 15 min in sodium barbital buffer containing 20 mM sodium barbital and 36 mM CaCl 2 at pH 10.2 and then incubated for 45 min in sodium barbital buffer containing 3.5 mM ATP, 20 mM sodium barbital, and 18 mM CaCl 2 at pH 9.4.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Cross-sections of 8 μm thick were cut and mounted on Fisher Superfrost Plus slides (Thermo Fisher Scientific). Myosin-ATPase staining was performed as described 37 . Briefly, muscle sections were pre-incubated for 15 min in sodium barbital buffer containing 20 mM sodium barbital and 36 mM CaCl 2 at pH 10.2 and then incubated for 45 min in sodium barbital buffer containing 3.5 mM ATP, 20 mM sodium barbital, and 18 mM CaCl 2 at pH 9.4.…”
Section: Methodsmentioning
confidence: 99%
“…The NADH-TR staining was performed as previously described 37 . Briefly, muscle sections were incubated at 37 °C for 10 min and then with 1 mg/ml nitroblue tetrazolium (Sigma-Aldrich) and 0.4 mg/ml β-NADH (Sigma-Aldrich) in 50 mM Tris-HCl buffer, pH 7.3, at 37 °C for 30 min.…”
Section: Methodsmentioning
confidence: 99%
“…Myofibrillar ATPase staining with pre-incubation at pH 4.3 was used to identify fibre types IIa, IIx and IIb in TA and VM muscles. ATPase (pH 4.3) staining was performed as described in recent publications 77 79 . After staining, the sections were washed with several changes of tap water, dehydrated with ethanol, cleared in xylene, and mounted in glycerol-gelatin aqueous slide mounting medium (Sigma-Aldrich, USA).…”
Section: Methodsmentioning
confidence: 99%
“…Myosin-ATPase and NADH-TR staining of the EDL and SOL sections prepared above were conducted as described previously [ 12 ]. Briefly, for myosin-ATPase staining, muscle sections were pre-incubated for 5 min in a sodium barbital buffer containing 20 mM sodium barbital and 36 mM CaCl 2 at pH 4.21 and then incubated for 55 min in a sodium barbital buffer containing 3.5 mM ATP, 20 mM sodium barbital, and 18 mM CaCl 2 at pH 9.45.…”
Section: Methodsmentioning
confidence: 99%