Muscle Contraction and Force: the Importance of an Ancillary Network, Nutrient Supply and Waste Removal

Brüggemann, Dagmar; Risbo, Jens; Pierzynowski, Stefan; Harrison, Adrian P.

doi:10.3390/ijms9081472

Cited by 3 publications

(3 citation statements)

References 42 publications

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“…2-Ox, a metabolite of the Krebs cycle and a precursor to the main amino acid of collagen, hydroxyproline, is a substance with great protective potential, especially with regard to metabolism and the condition of the skeleton and the whole organism in general ( 9 ) . Several previous studies have shown that dietary 2-Ox could prevent systemic diseases, like osteoporosis or increases in cholesterol, as well as improve kidney and muscle function ( 5 , 13 , 28 – 30 ) . Other studies have demonstrated the anabolic properties of 2-Ox regarding amino acid and protein utilisation, energy expenditure, the maintenance of body mass, as well as acceleration of body mass gain.…”

Section: Discussionmentioning

confidence: 99%

“…2-Ox is a N scavenger and a source and precursor of glutamine, synthesised in human skeletal muscles, which improves and stimulates protein synthesis and inhibits protein degradation in skeletal muscles ( 9 ) . Thus, 2-Ox supplementation serves to enhance muscle development, as reviewed by Brüggemann et al ( 30 ) . However, several studies reviewed by Harrison & Pierzynowski ( 9 ) showed that 2-Ox administered orally stimulates collagen synthesis and bone formation.…”

Section: Discussionmentioning

confidence: 99%

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The effect of dietary administration of 2-oxoglutaric acid on the cartilage and bone of growing rats

et al. 2013

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2-Oxoglutaric acid (2-Ox), a precursor to hydroxyproline -the most abundant amino acid in bone collagen, exerts protective effects on bone development during different stages of organism development; however, little is known about the action of 2-Ox on cartilage. The aim of the present study was to elucidate the influence of dietary 2-Ox supplementation on the growth plate, articular cartilage and bone of growing rats. A total of twelve male Sprague -Dawley rats were used in the study. Half of the rats received 2-oxoglutarate at a dose of 0·75 g/kg body weight per d in their drinking-water. Body and organ weights were measured. Histomorphometric analyses of the cartilage and bone tissue of the femora and tibiae were conducted, as well as bone densitometry and peripheral quantitative computed tomography (pQCT). Rats receiving 2-Ox had an increased body mass (P,0·001) and absolute liver weight (P¼ 0·031). Femoral length (P¼ 0·045) and bone mineral density (P¼ 0·014), overall thickness of growth plate (femur P¼0·036 and tibia P¼0·026) and the thickness of femoral articular cartilage (P,0·001) were also increased. 2-Ox administration had no effect on the mechanical properties or on any of the measured pQCT parameters for both bones analysed. There were also no significant differences in histomorphometric parameters of tibial articular cartilage and autofluorescence of femoral and tibial growth plate cartilage. Dietary supplementation with 2-Ox to growing rats exerts its effects mainly on cartilage tissue, having only a slight influence on bone. The effect of 2-Ox administration was selective, depending on the particular bone and type of cartilage analysed.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The effect of dietary administration of 2-oxoglutaric acid on the cartilage and bone of growing rats

et al. 2013

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“…The labeled blocks were subsequently incubated in 3% porcine gelatin in PBS. A second fixation in 4% paraformaldehyde-PBS was introduced before the final immunolabeling procedure was performed (19). After incubation with anti-peptide scFv-phages, a mouse anti-M13 phage antibody (GE Healthcare, Chalfont St. Giles, United Kingdom) was added and incubated with the fluorescently labeled Alexa Fluor 647 donkey antimouse antibody (Molecular Probes, Invitrogen, Eugene, OR).…”

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confidence: 99%

A Novel Method forin SituDetection of Hydrolyzable Casein Fragments in a Cheese Matrix by Antibody Phage Display Technique and CLSM

Zhi

Brüggemann²,

Siegumfeldt³

2009

J. Agric. Food Chem.

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A novel method to monitor in situ hydrolyzable casein fragments during cheese ripening by using immunofluorescent labeling and confocal laser scanning microscopy (CLSM) was developed. Monoclonal single chain variable fragments of antibody (scFvs) were generated by antibody phage display toward three small synthetic peptides of the alpha(s1)-casein sequence. These peptides traverse enzymatic cleavage sites of casein during cheese ripening. The specificity of the generated anti-peptide antibodies was determined by ELISA and Western blot. Finally, an immunofluorescent labeling protocol was successfully developed for the detection of scFvs binding to different alpha(s1)-casein fragments inside a cheese matrix by CLSM. To our knowledge, this is the first demonstrated immunofluorescent labeling method for in situ analysis of proteolysis phenomena in the cheese matrix. Additionally, this technique offers a high potential to study in situ dynamic spatial changes of target components in complex food systems.

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Changes During Passive Recovery In Lower Limbs Tiredness After Strenuous Workout

Mondal¹,

Sarkar²

2013

IOSRJSPE

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Lower limbs tiredness is a widely accepted indicator for recovery state prediction. The study was designed and purposed to know the rate and trend of lower limbs tiredness recovery after strenuous workout in passive state. Ten athletes from LNIPE, Gwalior having almost similar anthropometric measurements, physiological capacity, chronological age(18-19 year), training age(5-6 year), event(sprinters) etc. residing in same campus having similar daily routine were selected as participant in this experiment. The experiment was conducted in a highly controlled environment using sophisticate equipments. Target Heart Rate Zone of the workout lasting for 20 minutes was 80%-90% of their Maximum Heart Rate. Three readings including pre, post and 30 minutes post workout was considered for both the two tests (Isometric Leg Strength Test and Sergeant Jump Test) selected for the purpose. rANOVA was employed separately to derive out meaningful information from the raw data. In both the tests well controlled workout for 20 minutes resulted in significant increase state of post workout readings. With passage of time after 30 minutes post passive recovery there was no improvement in state of tiredness. Thus scope of future research is there in planning out means and methods to promote lower limbs tiredness recovery during this post recovery period.

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Muscle Contraction and Force: the Importance of an Ancillary Network, Nutrient Supply and Waste Removal

Cited by 3 publications

References 42 publications

The effect of dietary administration of 2-oxoglutaric acid on the cartilage and bone of growing rats

The effect of dietary administration of 2-oxoglutaric acid on the cartilage and bone of growing rats

A Novel Method forin SituDetection of Hydrolyzable Casein Fragments in a Cheese Matrix by Antibody Phage Display Technique and CLSM

Changes During Passive Recovery In Lower Limbs Tiredness After Strenuous Workout

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