Muscarinic cholinoceptor activation modulates DNA synthesis and CD40 expression in fibroblast cells

Casanova, Marta; Furlan, César; Sterin‐Borda, Leonor; Borda, Enri

doi:10.1111/j.1474-8673.2006.00369.x

Cited by 6 publications

(7 citation statements)

References 47 publications

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“…The increase in the apoptotic process is provoked by an activation of fibroblast‐mAChR M 1 and M 3 subtypes. Previously, by pharmacological analysis, we had shown that M 1 and M 3 mAChR subtypes were important mediators of mAChR agonist‐induced biological effects on human skin fibroblasts (Casanova et al, 2006), while M 2 and M 4 seemed to have no relevance in these actions. Depending on the receptor subtype and the cell type in which the receptor is expressed, G‐protein coupled receptors (GPCRs) can either induce apoptosis or protect cells from apoptotic stimuli.…”

Section: Discussionmentioning

confidence: 99%

“…Human fibroblasts express muscarinic acetylcholine receptors (mAChRs) of different subtypes (Carsi‐Gabrenas et al, 1997; Buchli et al, 1999). We demonstrated that mAChRs are expressed in human skin fibroblasts and their activation by carbachol induced an increment in DNA synthesis and in CD40 expression (Casanova et al, 2006). Pharmacological analysis with different mAChR antagonists allowed us to identify which of the known mAChR subtypes are active in human skin fibroblasts.…”

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confidence: 91%

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Dynamic cell shapes and contacts in the developing Drosophila retina are regulated by the Ig cell adhesion protein hibris

Grillo-Hill

Wolff

2009

Developmental Dynamics

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Cell shapes and contacts are dynamically regulated during organogenesis to enable contacts with relevant neighboring cells at appropriate times. During Drosophila larval eye development, an apical contact is established between one pair of non-neuronal cones cells, precluding contact between the opposing pair. Concurrent with changes in cell shape, these contacts reverse in early pupal life. The reversal in cone cell contacts occurs in a posterior to anterior gradient across the eye, following the developmental gradient established in the larval eye imaginal disc. Hibris (Hbs), an Immunoglobulin cell adhesion molecule homologous to vertebrate Nephrin, is required for cone cell morphogenesis. In hbs null mutants, a majority of cone cells fail to both establish wild-type contacts and achieve mature cone cell shapes. hbs acts cell autonomously in the cone cells to drive these changes. The work presented here indicates hbs contributes to the remodeling of cell contacts and cell shapes throughout development. Developmental Dynamics 238: 2223-2234, 2009.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 91%

Dynamic cell shapes and contacts in the developing Drosophila retina are regulated by the Ig cell adhesion protein hibris

Grillo-Hill

Wolff

2009

Developmental Dynamics

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“…Whether these effects are involved in the pathogenesis of chronic airway diseases remains to be elucidated. In human neonatal skin fibroblasts, mAChRs stimulate DNA synthesis and CD40 expression ( Casanova et al ., 2006 ).…”

Section: Cellular Functions Of Non‐neuronal Achmentioning

confidence: 99%

Acetylcholine beyond neurons: the non‐neuronal cholinergic system in humans

Wessler

Kirkpatrick

2008

British J Pharmacology

742

659

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Animal life is controlled by neurons and in this setting cholinergic neurons play an important role. Cholinergic neurons release ACh, which via nicotinic and muscarinic receptors (n-and mAChRs) mediate chemical neurotransmission, a highly integrative process. Thus, the organism responds to external and internal stimuli to maintain and optimize survival and mood. Blockade of cholinergic neurotransmission is followed by immediate death. However, cholinergic communication has been established from the beginning of life in primitive organisms such as bacteria, algae, protozoa, sponge and primitive plants and fungi, irrespective of neurons. Tubocurarine-and atropine-sensitive effects are observed in plants indicating functional significance. All components of the cholinergic system (ChAT, ACh, n-and mAChRs, high-affinity choline uptake, esterase) have been demonstrated in mammalian non-neuronal cells, including those of humans. Embryonic stem cells (mice), epithelial, endothelial and immune cells synthesize ACh, which via differently expressed patterns of n-and mAChRs modulates cell activities to respond to internal or external stimuli. This helps to maintain and optimize cell function, such as proliferation, differentiation, formation of a physical barrier, migration, and ion and water movements. Blockade of n-and mACHRs on noninnervated cells causes cellular dysfunction and/or cell death. Thus, cholinergic signalling in non-neuronal cells is comparable to cholinergic neurotransmission. Dysfunction of the non-neuronal cholinergic system is involved in the pathogenesis of diseases. Alterations have been detected in inflammatory processes and a pathobiologic role of non-neuronal ACh in different diseases is discussed. The present article reviews recent findings about the non-neuronal cholinergic system in humans.

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“…Other studies indicated that carbachol and pilocarpine act on skin fibroblasts via M1 and M3 (23,24). Skin melanocytes express a1, a3, a5, a7, b1, b2, g, and d in nicotinic AChRs and all muscarinic AChRs (19).…”

Section: Skinmentioning

confidence: 97%

Non-neuronal regulation and repertoire of cholinergic receptors in organs

Sato

Chida

Iwata

et al. 2010

BioMolecular Concepts

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Many studies on the cholinergic pathway have indicated that cholinergic receptors, which are widely expressed in various cells, play an important role in all body organs. In this review, we present the concept that cholinergic responses are regulated through a neuronal or non-neuronal mechanism. The neuronal mechanism is a system in which acetylcholine binds to cholinergic receptors on target cells through the nerves. In the non-neuronal mechanism, acetylcholine, produced by neighboring cells in an autocrine/paracrine manner, binds to cholinergic receptors on target cells. Both mechanisms subsequently lead to physiological and pathophysiological responses. We also investigated the subunits/subtypes of cholinergic receptors on target cells, physiological and pathophysiological responses of the organs via cholinergic receptors, and extracellular factors that alter the subtypes/subunits of cholinergic receptors. Collectively, this concept will elucidate how cholinergic responses occur and will help us conduct further experiments to develop new therapeutic agents.

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Muscarinic cholinoceptor activation modulates DNA synthesis and CD40 expression in fibroblast cells

Cited by 6 publications

References 47 publications

Dynamic cell shapes and contacts in the developing Drosophila retina are regulated by the Ig cell adhesion protein hibris

Dynamic cell shapes and contacts in the developing Drosophila retina are regulated by the Ig cell adhesion protein hibris

Acetylcholine beyond neurons: the non‐neuronal cholinergic system in humans

Non-neuronal regulation and repertoire of cholinergic receptors in organs

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