Muscarinic agonist carbachol depresses excitatory synaptic transmission in the rat basolateral amygdala in vitro

Differential modulation by carbachol of four separate excitatory afferent systems to the rat subiculum in vitro

“…Other studies involving isolated synaptosomes (Marchi and Raiteri, 1989) or cultured hippocampal neurons (Segal, 1989) concluded that M 2 receptor activation was principally responsible. Finally, there are studies, which find, as we did, that synaptic depression results from the activation of multiple muscarinic receptor classes (Sugita et al, 1991;Yajeya et al, 2000). While all agree that the mechanism is presynaptic (see also de Sevilla et al, 2002), full agreement on the details has not been reached (e.g., Scanziani et al, 1995;Egorov et al, 1996).…”

“…Selective modulation of excitatory afferents by cholinergic receptor activation has been described in several hippocampal formation regions (Hasslemo et al, 2002), neocortex (e.g., Gil et al, 1997;Oldford and Castro-Alamancos, 2003), and subcortical regions such as amygdala (Sugita et al, 1991;Yajeya et al, 2000), nucleus accumbens (Sugita et al, 1991), and striatum (Sugita et al, 1991). The subiculum receives convergent inputs from CA1, entorhinal cortex and presubiculum to complete a variety of "long" and "short" loop neuronal circuits.…”

Differential modulation by carbachol of four separate excitatory afferent systems to the rat subiculum in vitro

“…Other studies involving isolated synaptosomes (Marchi and Raiteri, 1989) or cultured hippocampal neurons (Segal, 1989) concluded that M 2 receptor activation was principally responsible. Finally, there are studies, which find, as we did, that synaptic depression results from the activation of multiple muscarinic receptor classes (Sugita et al, 1991;Yajeya et al, 2000). While all agree that the mechanism is presynaptic (see also de Sevilla et al, 2002), full agreement on the details has not been reached (e.g., Scanziani et al, 1995;Egorov et al, 1996).…”

“…Selective modulation of excitatory afferents by cholinergic receptor activation has been described in several hippocampal formation regions (Hasslemo et al, 2002), neocortex (e.g., Gil et al, 1997;Oldford and Castro-Alamancos, 2003), and subcortical regions such as amygdala (Sugita et al, 1991;Yajeya et al, 2000), nucleus accumbens (Sugita et al, 1991), and striatum (Sugita et al, 1991). The subiculum receives convergent inputs from CA1, entorhinal cortex and presubiculum to complete a variety of "long" and "short" loop neuronal circuits.…”

A Cholinergic Synaptically Triggered Event Participates in the Generation of Persistent Activity Necessary for Eye Fixation

“…The ACSF was bubbled with carbogen gas (95% O 2 /5% CO 2 ) and maintained at 30 Ϯ 2°C. Additional details of this in vitro preparation were published previously (Yajeya et al, 2000).…”

Electrophysiological and Synaptic Characterization of Transplanted Neurons in Adult Rat Motor Cortex

“…Furthermore, carbachol modulated the evoked responses and RMP, suggesting a plausible double location for acetylcholine receptors as in other cortical or brainstem structures (Yajeya et al, 2000;Navarro-Lopez et al, 2004). In the presynaptic terminal they would adjust the amount of neurotransmitter to be liberated, and postsynaptically they would control membrane excitability.…”

“…Three months after transplantation the grafted rats (postnatal days ≥ 180) were processed to study the electrophysiological characteristics of the transplanted neurons in vitro (n = 38). The rats were deeply anesthetized with halothane gas, decapitated, and coronal slices (350 μm thick) containing the transplanted tissue were obtained as described previously (Yajeya et al, 2000). Briefly, slices were kept for at least 1 h at room temperature in aerated (95% O 2 + 5% CO 2 ) artificial cerebrospinal fluid (ACSF) before use.…”

Differential modulation by carbachol of four separate excitatory afferent systems to the rat subiculum in vitro

“…Other studies involving isolated synaptosomes (Marchi and Raiteri, 1989) or cultured hippocampal neurons (Segal, 1989) concluded that M 2 receptor activation was principally responsible. Finally, there are studies, which find, as we did, that synaptic depression results from the activation of multiple muscarinic receptor classes (Sugita et al, 1991;Yajeya et al, 2000). While all agree that the mechanism is presynaptic (see also de Sevilla et al, 2002), full agreement on the details has not been reached (e.g., Scanziani et al, 1995;Egorov et al, 1996).…”

“…Selective modulation of excitatory afferents by cholinergic receptor activation has been described in several hippocampal formation regions (Hasslemo et al, 2002), neocortex (e.g., Gil et al, 1997;Oldford and Castro-Alamancos, 2003), and subcortical regions such as amygdala (Sugita et al, 1991;Yajeya et al, 2000), nucleus accumbens (Sugita et al, 1991), and striatum (Sugita et al, 1991). The subiculum receives convergent inputs from CA1, entorhinal cortex and presubiculum to complete a variety of "long" and "short" loop neuronal circuits.…”

A Cholinergic Synaptically Triggered Event Participates in the Generation of Persistent Activity Necessary for Eye Fixation

“…The ACSF was bubbled with carbogen gas (95% O 2 /5% CO 2 ) and maintained at 30 Ϯ 2°C. Additional details of this in vitro preparation were published previously (Yajeya et al, 2000).…”

Electrophysiological and Synaptic Characterization of Transplanted Neurons in Adult Rat Motor Cortex

“…Furthermore, carbachol modulated the evoked responses and RMP, suggesting a plausible double location for acetylcholine receptors as in other cortical or brainstem structures (Yajeya et al, 2000;Navarro-Lopez et al, 2004). In the presynaptic terminal they would adjust the amount of neurotransmitter to be liberated, and postsynaptically they would control membrane excitability.…”

“…Three months after transplantation the grafted rats (postnatal days ≥ 180) were processed to study the electrophysiological characteristics of the transplanted neurons in vitro (n = 38). The rats were deeply anesthetized with halothane gas, decapitated, and coronal slices (350 μm thick) containing the transplanted tissue were obtained as described previously (Yajeya et al, 2000). Briefly, slices were kept for at least 1 h at room temperature in aerated (95% O 2 + 5% CO 2 ) artificial cerebrospinal fluid (ACSF) before use.…”

Differential modulation by carbachol of four separate excitatory afferent systems to the rat subiculum in vitro

“…Other studies involving isolated synaptosomes (Marchi and Raiteri, 1989) or cultured hippocampal neurons (Segal, 1989) concluded that M 2 receptor activation was principally responsible. Finally, there are studies, which find, as we did, that synaptic depression results from the activation of multiple muscarinic receptor classes (Sugita et al, 1991;Yajeya et al, 2000). While all agree that the mechanism is presynaptic (see also de Sevilla et al, 2002), full agreement on the details has not been reached (e.g., Scanziani et al, 1995;Egorov et al, 1996).…”

“…Selective modulation of excitatory afferents by cholinergic receptor activation has been described in several hippocampal formation regions (Hasslemo et al, 2002), neocortex (e.g., Gil et al, 1997;Oldford and Castro-Alamancos, 2003), and subcortical regions such as amygdala (Sugita et al, 1991;Yajeya et al, 2000), nucleus accumbens (Sugita et al, 1991), and striatum (Sugita et al, 1991). The subiculum receives convergent inputs from CA1, entorhinal cortex and presubiculum to complete a variety of "long" and "short" loop neuronal circuits.…”

A Cholinergic Synaptically Triggered Event Participates in the Generation of Persistent Activity Necessary for Eye Fixation

“…The ACSF was bubbled with carbogen gas (95% O 2 /5% CO 2 ) and maintained at 30 Ϯ 2°C. Additional details of this in vitro preparation were published previously (Yajeya et al, 2000).…”

Electrophysiological and Synaptic Characterization of Transplanted Neurons in Adult Rat Motor Cortex

“…Furthermore, carbachol modulated the evoked responses and RMP, suggesting a plausible double location for acetylcholine receptors as in other cortical or brainstem structures (Yajeya et al, 2000;Navarro-Lopez et al, 2004). In the presynaptic terminal they would adjust the amount of neurotransmitter to be liberated, and postsynaptically they would control membrane excitability.…”

“…Three months after transplantation the grafted rats (postnatal days ≥ 180) were processed to study the electrophysiological characteristics of the transplanted neurons in vitro (n = 38). The rats were deeply anesthetized with halothane gas, decapitated, and coronal slices (350 μm thick) containing the transplanted tissue were obtained as described previously (Yajeya et al, 2000). Briefly, slices were kept for at least 1 h at room temperature in aerated (95% O 2 + 5% CO 2 ) artificial cerebrospinal fluid (ACSF) before use.…”