Murine Lung Tumor Measurement Using Respiratory-Gated Micro-Computed Tomography

Cody, Dianna D.; Nelson, Christopher Lee; Bradley, William; Wislez, Marie; Juroske, Denise M.; Price, Roger E.; Zhou, Xian; Bekele, B. Nebiyou; Kurie, Jonathan M.

doi:10.1097/01.rli.0000160070.67270.05

Cited by 63 publications

(60 citation statements)

References 17 publications

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“…Kirsch et al, unpubl.). Similarly, micro-CT imaging of K-ras LA lung tumors revealed both tumor growth and shrinkage within individual mice (Cody et al 2005). These data make it clear that some lung tumors grow quickly, whereas others appear to progress with slower kinetics.…”

Section: Improving Murine Models: Cooperative Effects Of K-ras and P5mentioning

confidence: 74%

Mouse Models of Human Non-Small-Cell Lung Cancer: Raising the Bar

Kim¹,

Jackson²,

Kirsch³

et al. 2005

Cold Spring Harbor Symposia on Quantitative Biology

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Section: Improving Murine Models: Cooperative Effects Of K-ras and P5mentioning

confidence: 74%

Mouse Models of Human Non-Small-Cell Lung Cancer: Raising the Bar

Kim¹,

Jackson²,

Kirsch³

et al. 2005

Cold Spring Harbor Symposia on Quantitative Biology

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“…injection thrice a week for 3 weeks as previously described (15). Mice were subjected to respiratory-gated micro-computed tomography (micro-CT) at the beginning and end of the treatment (26), and they were killed by cervical dislocation within 6 hours of the final scan. During the autopsy, visible lesions were counted on the surfaces of both lungs by investigators (M.W.…”

Section: Methodsmentioning

confidence: 99%

“…Four-micrometer tissue sections were deparaffinized, rehydrated, and washed with PBS as described (26). Antigens were retrieved with 0.01 mol/L citrate buffer (pH 6; DakoCytomation) for 30 minutes in a steamer.…”

mentioning

confidence: 99%

High Expression of Ligands for Chemokine Receptor CXCR2 in Alveolar Epithelial Neoplasia Induced by Oncogenic Kras

Fujimoto²,

et al. 2006

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CXCL8, a ligand for the chemokine receptor CXCR2, was recently reported to be a transcriptional target of Ras signaling, but its role in Ras-induced tumorigenesis has not been fully defined. Here, we investigated the role of KC and MIP-2, the murine homologues of CXCL8, in Kras LA1 mice, which develop lung adenocarcinoma owing to somatic activation of the KRAS oncogene. We first investigated biological evidence of CXCR2 ligands in Kras LA1 mice. Malignant progression of normal alveolar epithelial cells to adenocarcinoma in Kras LA1 mice was associated with enhanced intralesional vascularity and neutrophilic inflammation, which are hallmarks of chemoattraction by CXCR2 ligands. In in vitro migration assays, supernatants of bronchoalveolar lavage samples from Kras LA1 mice chemoattracted murine endothelial cells, alveolar inflammatory cells, and the LKR-13 lung adenocarcinoma cell line derived from Kras LA1 mice, an effect that was abrogated by pretreatment of the cells with a CXCR2-neutralizing antibody. CXCR2 and its ligands were highly expressed in LKR-13 cells and premalignant alveolar lesions in Kras LA1 mice. Treatment of Kras LA1 mice with a CXCR2-neutralizing antibody inhibited the progression of premalignant alveolar lesions and induced apoptosis of vascular endothelial cells within alveolar lesions. Whereas the proliferation of LKR-13 cells in vitro was resistant to treatment with the antibody, LKR-13 cells established as syngeneic tumors were sensitive, supporting a role for the tumor microenvironment in the activity of CXCR2. Thus, high expression of CXCR2 ligands may contribute to the expansion of early alveolar neoplastic lesions induced by

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“…Extrathoracic metastases are rare and occur only at a very late stage of the disease, as in humans with adenocarcinoma (Wislez et al, 2003;Jonhson et al, 2001). The radiological appearance is also very similar to the human disease with multifocal lesions such as nodules with ground glass attenuation (Cody et al, 2005). We are presently using this model to analyze the administration of anticancer drugs via the airways.…”

Section: Transgenic Lung Cancermentioning

confidence: 97%