Multivariate statistical analysis of the polyphenols content for the discrimination of honey produced in Sicily (Southern Italy)

Moringa oleifera Protects SH-SY5YCells from DEHP-Induced Endoplasmic Reticulum Stress and Apoptosis

“…The two on-line columns Cyclone P column (50 mm ×0.5 m, 60 μm particle size, 60 Å pore size, Thermo Fisher Scientific, Waltham, MA, USA) and Hypersil Gold (2.1 × 100 mm, 1.7 μm particle size) were eluted by the same gradient program according toLópez-Gutiérrezet al and Lo Dico et al, with some modifications [ 45 , 47 ].The total run time was 10 min. Data analysis was performed using Thermo Scientific XCalibur (Thermo Fisher Scientific, Waltham, MA, USA) version 4.0 software and Qual Browser.…”

“…HO-1 catalyzes the conversion of heme to biliverdin, ferrous iron, and carbon monoxide, which can directly scavenge free radicals. Interestingly, our recent in vivo and in vitro studies with mushrooms polyphenols have demonstrated their neuroprotective effects by modulation of stress-responsive vitagenes in different brain regions of rats [ 43 , 44 , 45 ], as well as in pheochromocytoma 12 (PC12) cells against DEHP-induced neurotoxicity [ 46 ]. Hence, the present research was designed to examine the efficacy of MOe in protecting human neuroblastome cells against toxic effects induced by DEHP.…”

High hydroxycinnamic acids contents in fennel honey produced in Southern Italy

“…The honey samples were examined for floral origin by melissopalynological analysis, according to the protocol of Ohe et al (2004) by consulting the atlas of Ricciardelli d'Albore (1998). The samples were stored at controlled temperature and humidity conditions (about 25 C and 60% humidity) and protected from light and heat for the determination of polyphenols contents according to the protocol of Lo Dico et al (2019).…”

“…The polyphenols contents were assessed by a Q-Exactive Plus Hybrid Quadrupole-Orbitrap TM Mass Spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) equipped with an HESI (Heated Electro Spray Ionization) configured at positive and negative polarity modes. The chromatographic and Mass spectrometer conditions were according to Lo Dico et al (Lo Dico et al 2019). Briefly, the chromatographic conditions were as follow: column temperature, 30 C; sample temperature, 6 C; flow rate, 0.2 mL min À1 .…”

“…Several flavonoids present in honey, such as apigenin, myricetin, naringenin, quercetin, hesperetin and pinocembrin, due to their antioxidant activity, have neuroprotective, anti-inflammatory and anticarcinogenic properties. The characterisation and quantification of polyphenolic compounds and other components in honey, that might be responsible for their nutraceutical properties, are essential to improve our knowledge about the honey potential effects for the human health (Lo Dico et al 2019). Honey composition is closely related to its botanical origin and to the environmental conditions of the area (Manyi-Loh et al 2011).…”