Multistep regulation of enhancer activity of the 21-base-pair element of human T-cell leukemia virus type I

Abstract

We examined the regulatory mechanisms of binding and transcriptional enhancement of the 21-bp core element of the enhancer of human T-cell leukemia virus type I (HTLV-I) in response to forskolin, 12-0-tetradecanoylphorbol-13-acetate (TPA), and a viral transactivator, p4laX. The 21-bp core element has been shown to bind to a cyclic AMP-responsive element binding protein (CREB)-like molecule at the site of an imperfect palindrome containing the TGAC motif. Experiments with oligonucleotides with mutations in the …