Multipotent stem/progenitor cells in the human foetal biliary tree

Participation of peribiliary glands in biliary tract pathophysiologies

“…Carpino et al [12] also reported that pancreatic genes such as CFTR were weakly expressed in the peribiliary glands [11,13] . Thus, it seems possible that the peribiliary glands may have the 429 August 27, 2013|Volume 5|Issue 8| WJH|www.wjgnet.com potential to become pancreatic cells and the biliary tract can be regarded as an incomplete pancreas [1] .…”

“…Recent studies have shown multipotent, endoderm S/P cells to be present in human peribiliary glands at all ages [11][12][13] . In the studies using cell cultures and tissue explants, isolated cells of the peribiliary glands expanded in vitro and these cells readily and efficiently showed cell lineages differentiating into liver, the biliary tree or pancreatic cells [11][12][13] .…”

“…In the studies using cell cultures and tissue explants, isolated cells of the peribiliary glands expanded in vitro and these cells readily and efficiently showed cell lineages differentiating into liver, the biliary tree or pancreatic cells [11][12][13] . It was found in this study that the markers of endodermal S/P cells such as EpCAM, CD44s, CXCR4 and SOX9 [13,[21][22][23] were variably expressed in the peribiliary glands and also in the lining epithelium of hilar bile ducts, supporting that peribiliary glands are a niche for endodermal S/P cells [11][12][13] . The different expression patterns of endoderm S/P cell markers may reflect heterogeneous S/P cell components within the peribiliary glands.…”

“…Recently, the peribiliary glands were reported to be stem cell niches of the biliary tree [11] and these stem cells were shown to be capable of differentiating into hepatobiliary and pancreatic cells [11][12][13] . According to Carpino et al [12] , these peribiliary glands harbor stem/progenitor (S/P) cells of the liver, bile duct and pancreas, which express endodermal S/P cell markers, such as C-X-C chemokine receptor type 4 (CXCR4), PDX1, HES1, SOX9/17, epithelial cell adhesion molecule (EpCAM) and CD44s, and these cells also weakly express adult liver, bile duct and pancreatic markers, such as albumin and cystic fibrosis transmembrane conductance regulator (CFTR) [11][12][13] . These S/P cells in the peribiliary glands are likely to As positive controls, islet cells in normal pancreatic tissue (one case) for PDX1, fibroblasts in cirrhotic liver tissue (one case) for HES1, neural cells in a normal human brain (one case) for CXCR4, fibroblasts in cirrhotic liver tissue (one case) for CD44s, normal human embryonic tissue (one case) for SOX9, and bile ducts in a normal liver (one case) for EpCAM were used as shown in Table 1.…”

<b>Tratamento com células derivadas do fígado embrionário retarda a progressão da fibrose hepática em ratos</b>

“…Entretanto como nos animais tratados houve uma maior expressão desse marcador, os resultados referentes e a morfometria e à quantificação demonstraram maior expressão deste marcador nos animais do grupo G3 em relação ao grupo G2, confirmando a hipótese de maior estimulo a regeneração tecidual, considerando o potencial regenerativo das células (PAYUSHINA et al, 2013;PATIL et al, 2014) As células derivadas do fígado embrionário de ratos com 14,5 dias de gestação demonstraram as características esperadas para terapia, uma vez que tais células apresentaram marcadores de células progenitoras hepáticas tais como CD 44, CD 117(c-kit), Nestin (OERTEL, 2011;BIN et al, 2012;EXPERIMENTAL, 2013;GONGH et al, 2013), assim como CK 18, marcador de hepatoblastos precursores de hepatócitos (FIEGEL et al, 2006;SEMERARO et al, 2012;HABEEB et al, 2015). Quanto ao marcador de colangiocitos, CK 7 (CANTZ; MANNS; OTT, 2008;OERTEL et al, 2008;GONGH et al, 2013), este também foi expresso, confirmando assim o seu bipotencial de diferenciação com sugerem os trabalhos de (OERTEL, 2011;BIN et al, 2012;YOVCHEV et al, 2014).…”